Conserved nucleosome positioning defines replication origins
- 1Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA;
- 2Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusets 02139, USA
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↵3 These authors contributed equally to this work.
Abstract
The origin recognition complex (ORC) specifies replication origin location. The Saccharomyces cerevisiae ORC recognizes the ARS (autonomously replicating sequence) consensus sequence (ACS), but only a subset of potential genomic sites are bound, suggesting other chromosomal features influence ORC binding. Using high-throughput sequencing to map ORC binding and nucleosome positioning, we show that yeast origins are characterized by an asymmetric pattern of positioned nucleosomes flanking the ACS. The origin sequences are sufficient to maintain a nucleosome-free origin; however, ORC is required for the precise positioning of nucleosomes flanking the origin. These findings identify local nucleosomes as an important determinant for origin selection and function.
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Footnotes
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↵4 Corresponding author.
E-MAIL david.macalpine{at}duke.edu; FAX (919) 684-1666.
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Article published online ahead of print. Article and publication date are online at http://www.genesdev.org/cgi/doi/10.1101/gad.1913210.
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Supplemental material is available at http://www.genesdev.org.
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- Received August 6, 2009.
- Accepted March 3, 2010.
- Copyright © 2010 by Cold Spring Harbor Laboratory Press
