Gcn5 regulates the dissociation of SWI/SNF from chromatin by acetylation of Swi2/Snf2
- 1Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA;
- 2Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
Abstract
The positive link between the SWI/SNF and the Gcn5 histone acetyltransferase in transcriptional activation has been well described. Here we report an inhibitory role for Gcn5 in SWI/SNF targeting. We demonstrate that Gcn5-containing complexes directly acetylate the Snf2 subunit of the SWI/SNF complex in vitro, as well as in vivo. Moreover, the acetylation of Snf2 facilitates the dissociation of the SWI/SNF complex from acetylated histones, and reduces its association with promoters in vivo. These data reveal a novel mechanism by which Gcn5 modulates chromatin structure not only through the acetylation of histones, but also by directly acetylating Snf2.
Keywords
Footnotes
-
↵3 Corresponding author.
E-MAIL jlw{at}stowers.org; FAX (816) 926-4692.
-
Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.1979710.
-
Supplemental material is available for this article.
- Received August 9, 2010.
- Accepted November 1, 2010.
- Copyright © 2010 by Cold Spring Harbor Laboratory Press