The S. cerevisiae SET3 complex includes two histone deacetylases, Hos2 and Hst1, and is a meiotic-specific repressor of the sporulation gene program

  1. W.W.M. Pim Pijnappel1,6,
  2. Daniel Schaft1,6,
  3. Assen Roguev1,
  4. Anna Shevchenko2,
  5. Hille Tekotte4,
  6. Matthias Wilm2,
  7. Guillaume Rigaut2,
  8. Bertrand Séraphin5,
  9. Rein Aasland3, and
  10. A. Francis Stewart1,7
  1. 1Gene Expression Program, European Molecular Biology Laboratory, 69117 Heidelberg, Germany and Genomics, Technische Universitaet Dresden, 01307 Dresden, Germany; 2Instrumentation Program, European Molecular Biology Laboratory, 69117 Heidelberg, Germany; 3Department of Molecular Biology, University of Bergen, N-5020 Bergen, Norway

Abstract

Set3 is one of two proteins in the yeast Saccharomyces cerevisiae that, like Drosophila Trithorax, contains both SET and PHD domains. We found that Set3 forms a single complex, Set3C, with Snt1, YIL112w, Sif2, Cpr1, and two putative histone deacetylases, Hos2 and NAD-dependent Hst1. Set3C includes NAD-dependent and independent deacetylase activities when assayed in vitro. Homology searches suggest that Set3C is the yeast analog of the mammalian HDAC3/SMRT complex. Set3C represses genes in early/middle of the yeast sporulation program, including the key meiotic regulators ime2and ndt80. Whereas Hos2 is only found in Set3C, Hst1 is also present in a complex with Sum1, supporting previous characterizations of Hst1 and Sum1 as repressors of middle sporulation genes during vegetative growth. However, Hst1 is not required for meiotic repression by Set3C, thus implying that Set3C (−Hst1) and not Hst1–Sum1, is the meiotic-specific repressor of early/middle sporulation genes.

Keywords

Footnotes

  • Present addresses: 4Wellcome Trust Centre for Cell Biology, ICMB, King's Buildings, University of Edinburgh, Edinburgh EH9 3JR, Scotland; 5CGM-Centre National de la Recherche Scientifique, Cedex 91198, France.

  • ↵6 These authors contributed equally to this work.

  • ↵7 Corresponding author.

  • E-MAIL stewart{at}mpi-cbg.de; FAX 49-351-210-1409.

  • Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.207401.

    • Received May 7, 2001.
    • Accepted September 17, 2001.
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  1. doi: 10.1101/gad.207401 Genes & Dev. 15: 2991-3004 Cold Spring Harbor Laboratory Press

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