Regulation of nucleosome landscape and transcription factor targeting at tissue-specific enhancers by BRG1
- Gangqing Hu1,4,
- Dustin E. Schones1,4,5,
- Kairong Cui1,4,
- River Ybarra2,
- Daniel Northrup1,
- Qingsong Tang1,
- Luca Gattinoni3,
- Nicholas P. Restifo3,
- Suming Huang2 and
- Keji Zhao1,6
- 1Laboratory of Molecular Immunology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA;
- 2Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville, Florida 32611, USA;
- 3Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
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↵4 These authors contributed equally to this work.
Abstract
Enhancers of transcription activate transcription via binding of sequence-specific transcription factors to their target sites in chromatin. In this report, we identify GATA1-bound distal sites genome-wide and find a global reorganization of the nucleosomes at these potential enhancers during differentiation of hematopoietic stem cells (HSCs) to erythrocytes. We show that the catalytic subunit BRG1 of BAF complexes localizes to these distal sites during differentiation and generates a longer nucleosome linker region surrounding the GATA1 sites by shifting the flanking nucleosomes away. Intriguingly, we find that the nucleosome shifting specifically facilitates binding of TAL1 but not GATA1 and is linked to subsequent transcriptional regulation of target genes.
Footnotes
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↵6 Corresponding author.
E-mail zhaok{at}nhlbi.nih.gov.
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[Supplemental material is available for this article.]
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Article published online before print. Article, supplemental material, and publication date are at http://www.genome.org/cgi/doi/10.1101/gr.121145.111.
- Received January 20, 2011.
- Accepted June 21, 2011.
