Global analysis of transcript and protein levels across the Plasmodium falciparum life cycle
- Karine G. Le Roch1,2,6,
- Jeffrey R. Johnson1,6,
- Laurence Florens1,6,7,
- Yingyao Zhou2,
- Andrey Santrosyan2,
- Munira Grainger3,
- S. Frank Yan2,
- Kim C. Williamson4,
- Anthony A. Holder3,
- Daniel J. Carucci5,
- John R. Yates III1, and
- Elizabeth A. Winzeler1,2,8
- 1 Department of Cell Biology ICND202, the Scripps Research Institute, La Jolla, California 92037, USA
- 2 Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, USA
- 3 Division of Parasitology, National Institute for Medical Research, London NW7 1AA, United Kingdom
- 4 Department of Biology, Loyola University Chicago, Chicago, Illinois 60626, USA
- 5 Naval Medical Research Center, Malaria Program (IDD), Silver Spring, Maryland 20910, USA
Abstract
To investigate the role of post-transcriptional controls in the regulation of protein expression for the malaria parasite, Plasmodium falciparum, we have compared mRNA transcript and protein abundance levels for seven different stages of the parasite life cycle. A moderately high positive relationship between mRNA and protein abundance was observed for these stages; the most common discrepancy was a delay between mRNA and protein accumulation. Potentially post-transcriptionally regulated genes are identified, and families of functionally related genes were observed to share similar patterns of mRNA and protein accumulation.
Footnotes
-
[Supplemental material is available online at www.genome.org.]
-
Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.2523904.
-
↵6 These authors contributed equally to this work.
-
↵7 Present address: The Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA.
-
↵8 Corresponding author. E-mail winzeler{at}scripps.edu; fax (858) 784-9860.
-
- Accepted September 1, 2004.
- Received February 27, 2004.
- Cold Spring Harbor Laboratory Press
