Assembly, Verification, and Initial Annotation of the NIA Mouse 7.4K cDNA Clone Set

  1. Vincent VanBuren1,
  2. Yulan Piao1,
  3. Dawood B. Dudekula1,
  4. Yong Qian1,
  5. Mark G. Carter1,
  6. Patrick R. Martin1,
  7. Carole A. Stagg1,
  8. Uwem C. Bassey1,
  9. Kazuhiro Aiba1,
  10. Toshio Hamatani1,
  11. George J. Kargul1,
  12. Amber G. Luo1,
  13. Janet Kelso2,
  14. Winston Hide2, and
  15. Minoru S.H. Ko1,3
  1. 1Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging (NIA), National Institutes of Health, Baltimore, Maryland, 20892, USA; 2South African National Bioinformatics Institute, University of the Western Cape, Bellville, South Africa

Abstract

A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ∼120,000 cDNA clones, which were initially re-arrayed into a set of ∼11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.

[The sequence data from this study have been submitted to GenBank under accession nos. BQ550036–BQ563104.]

Footnotes

  • ↵3 Corresponding author.

  • E-MAIL kom{at}grc.nia.nih.gov; FAX (410) 558-8331.

  • Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.633802. Article published online before print in November 2002.

    • Received July 16, 2002.
    • Accepted September 11, 2002.
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  1. Published in Advance November 12, 2002, doi: 10.1101/gr.633802 Genome Res. 12: 1999-2003 Cold Spring Harbor Laboratory Press

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