An Enhanced Linkage Map of the Sheep Genome Comprising More Than 1000 Loci

  1. Jillian F. Maddox1,11,
  2. Kizanne P. Davies1,
  3. Allan M. Crawford2,
  4. Dennis J. Hulme3,
  5. Daniel Vaiman4,
  6. Edmond P. Cribiu4,
  7. Bradley A. Freking5,
  8. Ken J. Beh3,
  9. Noelle E. Cockett6,
  10. Nina Kang1,
  11. Christopher D. Riffkin1,
  12. Roger Drinkwater7,
  13. Stephen S. Moore7,
  14. Ken G. Dodds2,
  15. Joanne M. Lumsden2,
  16. Tracey C. van Stijn2,
  17. Sin H. Phua2,
  18. David L. Adelson3,
  19. Heather R. Burkin2,
  20. Judith E. Broom2,
  21. Johannes Buitkamp8,
  22. Lisa Cambridge2,
  23. William T. Cushwa10,
  24. Emily Gerard2,
  25. Susan M. Galloway2,
  26. Blair Harrison7,
  27. Rachel J. Hawken1,
  28. Stefan Hiendleder9,
  29. Hannah M. Henry2,
  30. Juan F. Medrano10,
  31. Korena A. Paterson2,
  32. Laurent Schibler4,
  33. Roger T. Stone5, and
  34. Beryl van Hest3
  1. 1Centre for Animal Biotechnology (CAB), The University of Melbourne, Parkville 3052, Australia; 2AgResearch Molecular Biology Unit, Department of Biochemistry and Centre for Gene Research, University of Otago, Dunedin, New Zealand; 3CSIRO Animal Production, Blacktown 2148, Australia; 4Institut National de la Recherche Agronomique (INRA), Department de Génétique Animale, 78350 Jouy-en-Josas, France; 5U.S. Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center, Nebraska 68933-0166, USA; 6Utah State University, Logan, Utah 84322–4700, USA; 7CSIRO Tropical Agriculture, St Lucia 4072, Australia; 8Lehrstuhl für Tierzucht, Technische Universität München, Freising, Germany; 9Department of Animal Breeding and Genetics, Justus-Liebig-University, D-35390 Giessen, Germany; 10Department of Animal Science, University of California, Davis, California 95616-8521, USA

Abstract

A medium-density linkage map of the ovine genome has been developed. Marker data for 550 new loci were generated and merged with the previous sheep linkage map. The new map comprises 1093 markers representing 1062 unique loci (941 anonymous loci, 121 genes) and spans 3500 cM (sex-averaged) for the autosomes and 132 cM (female) on the X chromosome. There is an average spacing of 3.4 cM between autosomal loci and 8.3 cM between highly polymorphic [polymorphic information content (PIC) ≥ 0.7] autosomal loci. The largest gap between markers is 32.5 cM, and the number of gaps of >20 cM between loci, or regions where loci are missing from chromosome ends, has been reduced from 40 in the previous map to 6. Five hundred and seventy-three of the loci can be ordered on a framework map with odds of >1000 : 1. The sheep linkage map contains strong links to both the cattle and goat maps. Five hundred and seventy-two of the loci positioned on the sheep linkage map have also been mapped by linkage analysis in cattle, and 209 of the loci mapped on the sheep linkage map have also been placed on the goat linkage map. Inspection of ruminant linkage maps indicates that the genomic coverage by the current sheep linkage map is comparable to that of the available cattle maps. The sheep map provides a valuable resource to the international sheep, cattle, and goat gene mapping community.

Footnotes

  • ↵11 Corresponding author.

  • E-MAIL jillm{at}rubens.its.unimelb.edu.au; FAX 61-3-9347-4083.

  • Article published on-line before print: Genome Res., 10.1101/gr. 135001. Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.135001.

    • Received March 7, 2001.
    • Accepted March 22, 2001.
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  1. Published in Advance June 12, 2001, doi: 10.1101/gr.135001 Genome Res. 11: 1275-1289 Cold Spring Harbor Laboratory Press

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