From DNA Sequence Analysis to Modeling Replication in the Human Genome

E. B. Brodie of Brodie, S. Nicolay, M. Touchon, B. Audit, Y. d’Aubenton-Carafa, C. Thermes, and A. Arneodo
Phys. Rev. Lett. 94, 248103 – Published 23 June 2005

Abstract

We explore the large-scale behavior of nucleotide compositional strand asymmetries along human chromosomes. As we observe for 7 of 9 origins of replication experimentally identified so far, the (TA+GC) skew displays rather sharp upward jumps, with a linear decreasing profile in between two successive jumps. We present a model of replication with well positioned replication origins and random terminations that accounts for the observed characteristic serrated skew profiles. We succeed in identifying 287 pairs of putative adjacent replication origins with an origin spacing 12Mbp that are likely to correspond to replication foci observed in interphase nuclei and recognized as stable structures that persist throughout subsequent cell generations.

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  • Received 19 November 2004

DOI:https://doi.org/10.1103/PhysRevLett.94.248103

©2005 American Physical Society

Authors & Affiliations

E. B. Brodie of Brodie1, S. Nicolay1, M. Touchon2, B. Audit1, Y. d’Aubenton-Carafa2, C. Thermes2, and A. Arneodo1

  • 1Laboratoire Joliot-Curie (CNRS), Ecole Normale Supérieure de Lyon, 46 Allée d’Italie, 69364 Lyon Cedex 07, France
  • 2Centre de Génétique Moléculaire (CNRS), Allée de la Terrasse, 91198 Gif-sur-Yvette, France

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Vol. 94, Iss. 24 — 24 June 2005

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