Multimode optical imaging of small animals: development and applications

J. Y. Hwang; C. Moffatt-Blue; O. Equils; M. Fujita; J. Jeong; N. M. Khazenzon; E. Lindsley; J. Ljubimova; A. G. Nowatzyk; D. L. Farkas; S. Wachsmann-Hogiu

doi:10.1117/12.711582

19 February 2007 Multimode optical imaging of small animals: development and applications

J. Y. Hwang, C. Moffatt-Blue, O. Equils, M. Fujita, J. Jeong, N. M. Khazenzon, E. Lindsley, J. Ljubimova, A. G. Nowatzyk, D. L. Farkas, S. Wachsmann-Hogiu

Author Affiliations +

Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 644105 (2007) https://doi.org/10.1117/12.711582
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We present an optical system for small animal imaging that can combine various in vivo imaging modalities, including fluorescence (intensity and lifetime), spectral, and trans-illumination imaging. This system consists of light-tight box with ultrafast pulsed or cw laser light excitation, motorized translational and rotational stages, a telecentric lens for detection, and a cooled CCD camera that can be coupled to an ultrafast time-gated intensifier. All components are modular, making possible laser excitation at various wavelengths and pulse lengths, and signal detection in a variety of ways (multimode). Results of drug nanoconjugate carrier delivery studies in mice are presented. Conventional and spectrally-resolved fluorescence images reveal details of in vivo drug nanoconjugate carrier accumulation within the tumor region and several organs in real time. By multi-spectral image analysis of ex vivo specimens from the same mice, we were able to evaluate the extent and topology of drug nanoconjugate carrier distribution into specific organs and the tumor itself.

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J. Y. Hwang, C. Moffatt-Blue, O. Equils, M. Fujita, J. Jeong, N. M. Khazenzon, E. Lindsley, J. Ljubimova, A. G. Nowatzyk, D. L. Farkas, and S. Wachsmann-Hogiu "Multimode optical imaging of small animals: development and applications", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 644105 (19 February 2007); https://doi.org/10.1117/12.711582

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