Abstract
A new potato virus X (PVX)-based viral vector for superproduction of target proteins in plants has been constructed. The triple gene block and coat protein gene of PVX were substituted by green fluorescent protein. This reduced viral vector was delivered into plant cells by agroinjection (injection of Agrobacterium tumefaciens cells, carrying viral vector cDNA within T-DNA, into plant leaves), and this approach allowed to dramatically reduce the size of the vector genome. The novel vector can be used for production of different proteins including pharmaceuticals in plants.
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Abbreviations
- CP:
-
coat protein
- crTMV:
-
crucifer infecting tobamovirus
- GFP:
-
green fluorescent protein
- MP:
-
movement protein
- PVA:
-
potato virus A
- PVX:
-
potato virus X
- RdRp:
-
RNA-dependent RNA-polymerase
- SGP:
-
subgenomic promoter
- sgRNA:
-
subgenomic RNA
- TBSV:
-
tomato bushy stunt virus
- T-DNA:
-
a fragment of Ti (tumor inducing) plasmid which is transferred into plant cell nucleus
- TGB:
-
triple gene block
- TMV:
-
tobacco mosaic virus
- 3′-UTR:
-
3′-untranslated region
- VIGS:
-
virus-induced gene silencing
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Published in Russian in Biokhimiya, 2006, Vol. 71, No. 8, pp. 1043–1049.
Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM06-109, July 9, 2006.
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Komarova, T.V., Skulachev, M.V., Zvereva, A.S. et al. New viral vector for efficient production of target proteins in plants. Biochemistry (Moscow) 71, 846–850 (2006). https://doi.org/10.1134/S0006297906080049
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DOI: https://doi.org/10.1134/S0006297906080049