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Characterization of glycoprotein E C-End of West Nile virus and evaluation of its interaction force with αVβ3 integrin as putative cellular receptor

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Abstract

Recombinant polypeptide containing the 260–466 amino acid sequence of West Nile virus (WNV) strain LEIV-Vlg99-27889-human glycoprotein E (gpE, E260–466) was constructed. Immunochemical similarity between the E260–466 and gpE of WNV was proven by enzyme immunoassay (EIA), immunoblot, competitive EIA, hemagglutination inhibition, and neutralization tests using polyclonal and monoclonal antibodies against the viral gpE and recombinant E260–466. Polypeptide E260–466 induced formation of virus neutralizing and cross-reactive antibodies that were interactive with various epitopes of this recombinant protein. It is shown by evaluation of the interaction of E260–466 with one of the proposed cell receptors of WNV that average E260–466-αVβ3 integrin-specific interaction force measured using atomic force spectroscopy was 80 and 140 pN for single and double interactions, correspondingly. Taken together with previously described interaction between laminin-binding protein (LBP) and WNV gpE domain II, it is proposed that WNV gpE can interact specifically with two cellular proteins (LBP and αVβ3 integrin) during virus entry.

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Abbreviations

AFM:

atomic force microscopy

EIA:

enzyme immunoassay

gpE:

glycoprotein E

LBP:

laminin-binding protein

MAb:

monoclonal antibodies

JEV:

Japanese encephalitis virus

RMSD:

root mean square deviation

TBEV:

tick-borne encephalitis virus

WNF:

West Nile fever

WNV:

West Nile virus

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Authors and Affiliations

  1. FSRI State Research Center of Virology and Biotechnology “Vector”, 630559, Koltsovo, Novosibirsk Region, Russia

    M. V. Bogachek, B. N. Zaitsev, E. V. Protopopova, V. A. Ternovoi, A. V. Ivanova, A. V. Kachko & V. B. Loktev

  2. Laboratoire de Physique de la Matiere Vivante, IPSB, BSP, Ecole Polytechnique Federale de Lausanne, CH 1015, Lausanne, Switzerland

    S. K. Sekatskii & G. Dietler

  3. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, ul. Lavrentieva 10, 630090, Novosibirsk, Russia

    V. A. Ivanisenko

Authors
  1. M. V. Bogachek
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  2. B. N. Zaitsev
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  3. S. K. Sekatskii
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  4. E. V. Protopopova
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  5. V. A. Ternovoi
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  6. A. V. Ivanova
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  7. A. V. Kachko
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  8. V. A. Ivanisenko
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  9. G. Dietler
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  10. V. B. Loktev
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Corresponding author

Correspondence to V. B. Loktev.

Additional information

Published in Russian in Biokhimiya, 2010, Vol. 75, No. 4, pp. 571–581.

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Bogachek, M.V., Zaitsev, B.N., Sekatskii, S.K. et al. Characterization of glycoprotein E C-End of West Nile virus and evaluation of its interaction force with αVβ3 integrin as putative cellular receptor. Biochemistry Moscow 75, 472–480 (2010). https://doi.org/10.1134/S0006297910040115

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  • DOI: https://doi.org/10.1134/S0006297910040115

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