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Transformation of wheat with the HMW-GS 1Bx14 gene without markers

  • Plant Genetics
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Abstract

High molecular weight (HMW) glutenin polypeptides are critical contributors to the visco/elastic properties responsible for the processing characteristics and utilizations of wheat flour. In order to improve bread making quality of flour and produce transgenic plants free of selectable markers, a linear DNA construct consisting of a minimal expression cassette with the HMW-GS 1Bx14 gene was transformed into wheat cultivar Mianyang19 by microprojectile bombardment. The transform ants were selected by PCR instead of herbicidal markers. Seven transgenic plants were identified from a total of 1219 transformants, yielding a transformation frequency of 0.28%. An SDS-PAGE analysis confirmed that the 1Bx14 gene was expressed in three T1 seeds of the transgenic plants. Our results demonstrated that it is feasible to obtain marker-free trans-formants using the linear-expression-cassette-transformation approach coupled with PCR selection.

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Authors and Affiliations

  1. College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China

    X. Liu, W. Jin, J. Liu, H. Zhao & A. Guo

  2. Key Laboratory for Molecular Biology of Agriculture, Shaanxi, 712100, China

    X. Liu, W. Jin & A. Guo

Authors
  1. X. Liu
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  2. W. Jin
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  3. J. Liu
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  4. H. Zhao
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  5. A. Guo
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Correspondence to A. Guo.

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X. Liu and W. Jin contributed equally to this work.

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Liu, X., Jin, W., Liu, J. et al. Transformation of wheat with the HMW-GS 1Bx14 gene without markers. Russ J Genet 47, 182–188 (2011). https://doi.org/10.1134/S1022795411010066

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  • DOI: https://doi.org/10.1134/S1022795411010066

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