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Modulation of Monocyte Chemotactic Protein-1 Expression During Lipopolysaccharide-Induced Preterm Delivery in the Pregnant Mouse

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Abstract

Preterm delivery is often associated with increased cytokine and chemokine production. These studies characterize the expression of the chemokine monocyte chemotactic protein-1 (MCP-1) in mice during lipopolysaccharide (LPS)—induced preterm delivery. Uterine and other tissues were harvested from CD-1 mice on gestational day 15 after intrauterine LPS injection. Quantitative real-time reverse-transcriptase polymerase chain reactions determined MCP-1 and toll-like receptor 4 (TLR4) mRNA expression during the 24 hours after LPS. MCP-1 protein expression was determined using a cytokine/chemokine protein array, enzyme-linked immunosorbant assay, and immunohistochemistry. Intrauterine LPS injection caused preterm delivery in CD-1 mice between 12 and 24 hours. Expression of MCP-1 mRNA significantly increased at 2 and 6 hours, while TLR4 expression did not significantly change over 24 hours. The MCP-1 protein levels peaked by 2 to 6 hours in maternal serum, liver, lung, kidney, and uterus. Immunohistochemistry confirmed MCP-1 in the myometrium and endometrium. These studies provide evidence suggesting that MCP-1 potentially plays an important role during the proinflammatory immune response, leading to preterm labor in the mouse.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, University of Vermont College of Medicine, Burlington, 89 Beaumont Avenue, VT, 05405, USA

    Allaire K. Diamond MEd, Leigh M. Sweet BA, Karen H. Oppenheimer BA & Mark Phillippe MD, MHCM

  2. Huntsman Cancer Institute, University of Utah, Salt Lake City, USA

    Diana F. Bradley BS

Authors
  1. Allaire K. Diamond MEd
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  2. Leigh M. Sweet BA
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  3. Karen H. Oppenheimer BA
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  4. Diana F. Bradley BS
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  5. Mark Phillippe MD, MHCM
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Corresponding author

Correspondence to Mark Phillippe MD, MHCM.

Additional information

Supported by the National Institute of Child Health and Human Development (grant HD 44747).

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Diamond, A.K., Sweet, L.M., Oppenheimer, K.H. et al. Modulation of Monocyte Chemotactic Protein-1 Expression During Lipopolysaccharide-Induced Preterm Delivery in the Pregnant Mouse. Reprod. Sci. 14, 548–559 (2007). https://doi.org/10.1177/1933719107307792

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  • DOI: https://doi.org/10.1177/1933719107307792

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