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A genomic glimpse of aminoacyl-tRNA synthetases in malaria parasite Plasmodium falciparum

BMC Genomics volume 10, Article number: 644 (2009) Cite this article

Abstract

Background

Plasmodium parasites are causative agents of malaria which affects >500 million people and claims ~2 million lives annually. The completion of Plasmodium genome sequencing and availability of PlasmoDB database has provided a platform for systematic study of parasite genome. Aminoacyl-tRNA synthetases (aaRS s) are pivotal enzymes for protein translation and other vital cellular processes. We report an extensive analysis of the Plasmodium falciparum genome to identify and classify aaRSs in this organism.

Results

Using various computational and bioinformatics tools, we have identified 37 aaRS s in P. falciparum. Our key observations are: (i) fraction of proteome dedicated to aaRS s in P. falciparum is very high compared to many other organisms; (ii) 23 out of 37 Pf-aaRS sequences contain signal peptides possibly directing them to different cellular organelles; (iii) expression profiles of Pf-aaRSs vary considerably at various life cycle stages of the parasite; (iv) several PfaaRSs posses very unusual domain architectures; (v) phylogenetic analyses reveal evolutionary relatedness of several parasite aaRS s to bacterial and plants aaRSs; (vi) three dimensional structural modelling has provided insights which could be exploited in inhibitor discovery against parasite aaRSs.

Conclusion

We have identified 37 Pf-aaRSs based on our bioinformatics analysis. Our data reveal several unique attributes in this protein family. We have annotated all 37 Pf-aaRSs based on predicted localization, phylogenetics, domain architectures and their overall protein expression profiles. The sets of distinct features elaborated in this work will provide a platform for experimental dissection of this family of enzymes, possibly for the discovery of novel drugs against malaria.

Background

Aminoacylation is the process of adding an aminoacyl group to the 3' end (CCA) of the tRNA molecule. tRNA is aminoacylated with a specific amino acid by aminoacyl-tRNA synthetase (aaRS s). aaRS s are responsible for attaching correct amino acid onto the cognate tRNA molecule in a two-step reaction. The amino acid is first activated with ATP forming an aminoacyladenylate intermediate. Once activated, this amino acid is transferred to the 3' end of its corresponding tRNA molecule to be processed during protein synthesis. All aaRSs require divalent cation MgCl2 for their aminoacylation reaction [1, 2].

Reaction:

  1. 1.

    amino acid + ATP → aminoacyl-AMP + PPi

  2. 2.

    aminoacyl-AMP + tRNA → aminoacyl-tRNA + AMP

The aaRS s are divided into two major classes based on structural topology of their active sites. Class I aaRS s represent 11 amino acids, including Arg, Cys, Gln, Glu, Ile, Leu, Lys, Met, Val, Trp and Tyr. Class II aaRS s includes 10 amino acids - Ala, Asp, Asn, Gly, His, Lys, Phe, Pro, Ser and Thr. Core domains of class I enzymes are characterized by a Rossmann fold which consists of α-helices and β-pleated sheets. This domain contains two conserved motifs ('HIGH' and 'KMSKS') which are directly involved in ATP binding. Catalytic domain of class II enzymes has a unique fold with a central core of anti-parallel β strands flanked by α helices [3]. There are three weakly conserved motifs, two of them are involved in ATP binding while the third one plays a role in homo dimerization. Class I enzymes bind ATP in an extended conformation while class II do so in a bent conformation. The two aaRS classes have different modes of aminoacylation - class I enzymes aminoacylate the 2'OH of the cognate tRNA whereas class II enzymes aminoacylate 3'OH of the tRNA (with the exception of PheRS) [4]. All known aaRS s are multidomain proteins with complex modular architectures [5]. In addition, eukaryotic aaRSs are distinguished by the presence of appended domains at either the N- or C-terminus which are generally absent from their bacterial/archaeal counterparts [6]. These appendages to the catalytic cores of several aaRSs are non-catalytic and instead function to mediate protein- protein interactions or act as general RNA-binding domains [7–9].

In mammalian cells, some aaRS s are present as a larger multi- aaRS complex (MSC) composed of nine synthetases (arginyl-, aspartyl-, glutamyl-, glutaminyl-, leucyl-, lysyl-, isoleucyl-, methionyl- and prolyl-tRNA synthetases) [10–12]. The MSC is composed of a mixture of class I and class II aaRS s along with three non- aaRS proteins p38, p43 and p18. It is not clear why certain aaRS s exist as a complex while some are in free form. MSC might help in efficient protein synthesis by preventing mixing of charged tRNAs with cellular pool and by increasing local concentration of tRNA near the site of protein synthesis [13].

The accuracy of tRNA aminoacylation reaction is critical in ensuring fidelity in protein translation [14]. To achieve this accuracy, some aaRS enzymes possess a proofreading (editing) mechanism that hydrolyzes tRNAs aminoacylated with the non-cognate amino acid [15]. For example, editing domains may be found attached to alanyl-tRNA synthetase (AlaRS), leucyltRNA synthetase (LeuRS) and so on [16–21]. In other cases, the editing domain is not attached to aaRS but rather functions as an individual protein [22, 23]. For example, YbaK protein from Haemophilus influenza is capable of efficiently editing Cys-tRNAPro[24]. ThrRS has been shown to have another editing domain called NTD which can cleave the bond between D-amino acid and tRNA [25].

Recently it has been shown that aaRS s are not only involved in protein synthesis but also perform many non-catalytic and non-canonical roles in RNA processing/trafficking, apoptosis, rRNA synthesis, angiogenesis and inflammation [26–30]. These versatile properties of aaRS s are the outcome of their differential cellular localization, nucleic acid binding properties, protein-protein interactions and collaboration (fusion) with additional domains. In case of malaria parasite, apicoplast proteins and pathways have already received particular attention as drug targets [31]. In this work we present a study of aaRS s from P. falciparum - the most virulent agent of human malaria. Our aim for this study was to use bioinformatics tools to (a) discover special and unusual modules present in parasite aaRSs which are potentially absent from human homologues, and (b) to identify potential new drug targets based on this protein family.

Results and Discussion

Sequence extraction and analysis

We exploited current annotation available in PlasmoDB [32] to identify the repertoire of aaRS s in P. falciparum genome. According to Enzyme Commission (EC) 37 proteins in PlasmoDB (see additional file 1) are annotated as belonging to the EC group 6.1.1. (EC number provided for aaRS s). Although in many cases current annotations allow an assignment to Class I or II of aaRS s, for some annotations are still preliminary. Due to this, we used Hidden Markov Models (HMMs) for identifying aaRSs in P. falciparum. For each aaRS a set of known sequences was utilized to construct 20 HMMs (see methods for details). For each database search a score distribution was obtained and 4 cutoffs were considered to identify aaRS. Results are reported in Table 1. We observed that 2 proteins annotated as belonging to EC group 6.1.1.- in PlasmoDB are not found by HMMs - PF14_0401 annotated as MetRS is instead a generic tRNA binding protein as elucidated in the genome re-annotation process, while the second one (PFC0470w) is still mis-annotated as ValRS. A total of 18 Pf-aaRS s can be classified within the 10 aaRS s that define class I. All members of this class are represented in the P. falciparum proteome. The annotations of these sequences are summarized in additional file 1. Similar to class I Pf-aaRSs, the class II Pf-aaRS s have a total of 18 sequences for 10 different amino acid synthetases. Four genes are present in P. falciparum for PheRS but these likely encode for 1 heterodimeric and 2 monomeric versions of PheRS.

Table 1 Results of database searches by HMM models of aaRS@.
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In order to carry out comparative analyses of aaRS s of P. falciparum with those of other species we considered aaRS sequences from several organisms representing three domains of life (see methods section). As expected, we found variable number of aaRS s in different species. M. jannaschii (archaebacteria) and M. tuberculosis (bacteria) have the lowest aaRS s count amongst other organisms like E. coli, S. cerevisiae, D. discoidium, P. falciparum, O. sativa, R. norvegicus, D. melanogaster, and H. sapiens. Human bears the highest number of aaRS s in this analysis (Figure 1a). Our analysis also shows that P. falciparum has the highest aaRS fraction (relative to its proteome size) when compared with bacteria, yeast and human counterparts (Figure 1b). The number of individual aaRS varies in different species. For example, when individual aaRS s from human and P. falciparum were compared it was evident that AlaRS and ThrRS were higher in number in humans (Figure 2). Presence of more than one copy of each aaRS in an organism may indicate additional biological, temporal or spatial roles for these enzymes as several aaRS s also perform non-canonical functions [33]. In this work we describe in detail the 37 Pf-aaRS s.

Figure 1
figure 1

(a) Predictied number of aaRS s present in Plasmodium falciparum (Pf), Rattus norvegicus (Rn), Saccharomyces cerevisiae (Sc), Drosophila melanogaster (Dm), Homo sapiens (Hs), Oryza sativa (Os), Dictyostelium discoidium (Dd), Mycobacterium tuberculosis (Mtb), Escherichia coli (Ec) , and Methanocalclococcus jannaschii (Mj). (b) Diagram representing fraction of proteome (in percentage) dedicated to the aaRS proteins in various organisms.

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Figure 2
figure 2

Bar graph showing number of different aaRSs in Plasmodium falciparum and Homo sapiens. The number of alanyl- and threonyl- tRNA synthetases is higher in humans whereas P. falciparum seems richer in phe tRNA synthetases.

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Indirect pathways of aminoacylation

It was earlier believed that 20 aaRS s were necessary for the incorporation of 20 amino acids in proteins. But surprisingly, some archaea, bacteria and chloroplasts lack GlnRS and AsnRS enzymes [34–38]. Interestingly, these organisms use an alternate pathway based on tRNA dependent amino acid transformation. A non-discriminating GluRS charges tRNAGln with glutamic amino acid and then a second enzyme called tRNA-dependent amidotransferase (AdT) amidates glutamate to make glutamine. A corresponding reaction occurs in case of asparagine residues. In case of P. falciparum, occurrence of glutamate-tRNA synthetase (PF13_0257, MAL13P1.281) and amidotransferase subunit A (PFD0780w) & subunit B (PFF1395c) together indicates presence of both direct and indirect pathways for aminoacylation [39, 40]. Both subunits of amidotransferase have apicoplast targeting signals suggesting an indirect pathway for aminoacylation in P. falciparum apicoplast. The expression of Pf-AdT subunit A is predicted in all life cycle stages of parasite based on proteomic and microarray data. We therefore feel that this pathway must also be active in the parasite apicoplast. We could not find sequence homologues of enzymes involved in indirect aminoacylation of cysteine residues [41–43] in the proteome of P. falciparum.

The multi-synthetase complex (MSC)

In mammalian cells, some aaRS s are present as a larger multi-aaRS complex (MSC). A constituent of the MSC - protein p43 - has sequence homologue (PF14_0401 - EMAP-II-like cytokine) in P. falciparum although there is no evidence for presence of MSC in malaria parasites. Interestingly, p43 is not only required for stability of the MSC complex but also functions as a proinflammatory cytokine [44–46]. Role of p43 homolog in P. falciparum is unknown, but evidence from other organisms indicates that MSC functions in protein stability, efficient protein translation and protein elongation [47]. Sequence identity between P. falciparum p43 and its human homolog is ~24% and based on microarray data p43 seems to be expressed at asexual life cycle stages of P. falciparum. A mitochondrial targeting signal was also predicted for parasite p43 but the role of p43 in parasite remains to be explored experimentally.

Targeting of aaRSs in the parasite

aaRS s are not only involved in protein synthesis but also in various other cellular activities including intron splicing, translational regulation and tRNA channeling. Diversified roles for aaRS s necessitate their presence (transit) into various cellular compartments. We therefore analyzed P. falciparum aaRS sequences for presence of putative signal sequences predicted by MITOPROT, PredictNLS and PATS for mitochondria, nucleus and apicoplast respectively. We found that 23 P. falciparum aaRS s have signal peptides, possibly for directing them to different cellular organelles. Another 14 aaRSs from P. falciparum may be resident in the parasite cytoplasm (Figure 3a). Apicoplast is known to have protein synthesis machinery which may use aaRS s [48]. Trafficking of nuclear encoded aaRS s to the apicoplast may explain why ~20 out of 37 Pf-aaRSs have apicoplast targeting signals. Our data indicate that out of total ~20 Pf-aaRSs bearing apicoplast targeting signals, ~12 aaRS s may be exclusive to this organelle. Others are predicted to be shared between apicoplast, nucleus and mitochondria (Figure 3b). It has been earlier shown that some tRNAs need to be aminoacylated in the nucleus before they can be exported to the cytoplasm, an observation indicating occurrence of aminoacylation reaction (mediated by aaRSs) inside the nucleus [49]. In P. falciparum, we found 10 aaRS s with nuclear localization signals but only one is predicted to be exclusively resident in the nucleus (PFA0480w- PheRS). Interestingly, we found no Pf-aaRS sequences with specific PEXEL (Plasmodium export element) motifs. This motif is found in parasite proteins that are exported beyond the parasitophorous vacuole membrane [50, 51].

Figure 3
figure 3

(a) Percentage predicted distribution of Pf - aaRSs in different organelles within the parasite. (b) A schematic of all Pf-aaRS s and their predicted cellular localization. Detailed information regarding gene IDs can be found in additional file 1. Pf-aaRS s predicted to be common between apicoplast & mitochondria, mitochondria & nucleus and apicoplast & nucleus are marked with diamond, triangle and square shapes respectively.

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Expression profiles of P. falciparum aaRSs

In order to study expression of aaRS during life cycle of the malaria parasite, we took advantage of available transcriptomics and proteomics data from PLASMODB. Firstly, we analyzed proteomic data from several independent experiments and compared them with transcriptomics data by Le Roch [52]. The latter sets of data were obtained using the affimetrix technology and hence provide a quantitative measure of mRNA levels in the parasite. Our results are provided in Table 2. Interestingly, we found that mRNA levels of potential apicoplast proteins (AP in the table) are lower on average (mean1 = 44.6; mean2 = 41.5; gam = 91.3; spor = 58.1) than those of potential cytoplasmic proteins (mean1 = 259; mean2 = 264.8; gam = 174.8; spor = 73.8). Proteomic data confirmed that while the cytoplasmic aaRS are found in almost all stages, the apicoplast aaRS are rarely found in the parasite. This could be in part due to experimental limits in the identification of apicoplast proteins by mass spectrometry. Indeed, when we carried out a chi-quadro test we found that proteins predicted to be targeted to apicoplast are significantly less represented (p < 10-4) in the sample of proteins identified by mass spectrometry. For these reasons we limited analysis of gene expression profiles only for putative cytoplasmic proteins. We considered trascriptomics data for sexual stages and asexual stages [52, 53]. We considered a reduced set of the time course gene expression data (22 time points instead of 48) and normalized data by Le Roch (see methods for details). This allowed us to analyse the expression of aaRS genes along all the intra-erythrocytic life cycle of the parasite (Table 2). Further observations of the protein expression profiles indicated that some aaRSs were exclusively detected at specific stages like, LeuRS (PF08_0011) and AspRS (PFE0715w) in sporozoites; IleRS (PFL1210w), SerRS (PF07_0073), GlnRS (PF13_0170), HisRS (PF14_0428) and PheRS (PFA0480w) in merozoites; AsnRS (PFE0475w), PheRS (PF11_0051) and HisRS (PFI1645c) in trophozoites and TrpRS (PF13_0205) in gametocyte stages (Figure 4).

Figure 4
figure 4

Diagrammatic representation of Pf-aaRS protein expression which are specifically expressed in different life stages of the parasite based on mass spectrometry data [82].

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Table 2 Transcriptomic and proteomic data for aaRSs in P. falciparum@
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Domain architecture of P. falciparum aaRSs

aaRS s are multi-domain proteins typically consisting of a conserved catalytic domain and an anti-codon binding domain. In addition, some aaRS s have RNA binding and editing domains that cleave incorrectly aminoacylated tRNA molecules [54]. Additional functional domains may be appended to aaRS s in the course of biological evolution [55, 56]. Careful examination of 37 identified P. falciparum aaRS s using Pfam database showed that most of them have a generic modular architecture that adheres to prototypical aaRSs (Figure 5). The remaining P. falciparum aaRS s or related proteins like PF14_0423 (protein having serine-threonine kinase domain in fusion with an anti-codon binding module) have complex domain architectures. In several, concatenation of unusual domains such as Ybak, GST, Ser-Thr kinase and DNA binding domains is evident (Figure 5). The functional relevance of these additional domains fused to typical aaRS in P. falciparum needs to be experimentally addressed. Intriguingly, two of the four Pf-PheRS subunits contain DNA binding domains (PF11_0051, PFA0480w). It is likely that the PheRS, in addition to its aminoacylation function, influences other cellular processes via DNA binding [57]. Consistent with its potential DNA binding property, the P. falciparum PheRS (PFA0480w) has a nuclear localization signal. The CysRS of B. subtilis (which also contains a DNA binding domain) is believed to play a role in initiating chromosomal replication [58]. Therefore, functional roles for P. falciparum PheRSs may extend from aminoacylation to DNA recognition and replication - a suggestion that requires experimental investigation. Similarly, it has been shown that GST or GST homology domains can help in complex formation of aaRS s with multifunctional factors (p38, p18) [56, 57]. Additional data show that deletion of GST homology domain from the C-terminal region of p38 results in the dissociation of EPRS (Glutamyl-prolyl-tRNA synthetase) and MetRS from the MSC complex [59]. Mammalian ValRS associated with elongation factor subunits also contain the GST homology domain [60–62]. Thus, the presence of GST domains might be a crucial feature of aaRS s. P. falciparum proteome has two such proteins with GST domains appended to MetRS (PF10_0340) and GluRS (PF13_0257). We also found a most interesting fusion of anticodon binding domain with a serine-threonine kinase (PF14_0423) in P. falciparum. This unusual kinase seems to be expressed throughout the life cycle of parasite (microarray data) and interestingly is predicted to be localized to the parasite nucleus. Clearly, the presence of unusual domain fusions in P. falciparum aaRS s suggests multiple functional roles for many of these P. falciparum enzymes as has been shown in other organisms.

Figure 5
figure 5

Representation of unusual domain architectures in Pf-aaRS s and related proteins. A generic aaRS is also shown on top. Domain name abbreviations are YB, Ybak associating domain; TS-II, class II tRNA synthetase; AC, anticodon binding site; ED, editing domain; GST, glutathione-Stransferase C-terminal region; RBD, S4 RNA binding domain; TS, tRNA synthetase core domain; STK, serine-threonine kinase; FTS, phenylalanine-tRNA synthetase; PTS, prolinetRNA synthetase; VTS, valine-tRNA synthetase; MTS, methionine-tRNA synthetase; YTS, tyrosine-tRNA synthetase; ETS, glutamate-tRNA synthetase.

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Phylogenetics

Overall the percentage identity between matching human and P. falciparum aaRS domains varies from 17 to 51. Clearly, Pf-aaRSs which have low sequence identity with human counterparts might serve as good drug targets. In order to study evolutionary relationships of P. falciparum aaRS s with other species, phylogenetic trees were developed in PHYML using maximum likelihood method. For each type of P. falciparum aaRS a separate tree was constructed (see additional file 2). aaRS sequences from 102 different species were used for multiple sequence alignments. As an example, phylogenetic tree of TyrRS from various species (including two sequences from P. falciparum) was constructed. Interestingly, one Pf-TyrRS (MAL8P1.125) clustered with human TyrRS whereas the second Pf-TyrRS (PF11_0181) clustered with bacterial TyrRS indicating different evolutionary origins (Figure 6a). Based on distance matrices, several P. f alciparum aaRS sequences clustered as being closer to plants (A. thaliana) or to bacteria (E. coli) (Figure 6b). It is already known that apicomplexan parasites like P. falciparum house a secondary endosymbiotic plastid, possibly hijacked by lateral genetic transfer from an alga. Therefore, the P. falciparum aaRS sequences which are evolutionary close to bacteria and plants are likely to be the outcome of horizontal gene transfer from the plastid. P. falciparum contains ~12 such aaRS sequences which cluster with bacterial or plant sequences. Functional and structural characterization of these bacterial/plant-like aaRS may be relevant in focusing efforts at using aaRS as drug targets.

Figure 6
figure 6

(a) Evolutionary tree was constructed using the PHYML based on maximum likelihood method. P. falciparum TyrRSs (PlasmoDB id -MAL8p1.125 and PF11_0181) are labeled as green triangles. One of the TryRSs (MAL8p1.125) is evolutionarily closer to H. sapiens whereas the other TyrRS (PF11_0181) is closer to E. coli. Total of 102 species were considered for the evolutionary analysis and were taken from three domains of life. (b) List of Pf-aaRS sequences evolutionarily closer to their E. coli and A. Thaliana counterparts.

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Homology modeling and structure comparisons

To date, no crystal structures have been obtained for any aaRS from P. falciparum. Hence, we performed homology modeling of several P. falciparum aaRSs using homologous structures available in PDB. Known structural templates (≥ 40% identity) were used for molecular modeling of several P. falciparum aaRSs including the two TyrRSs (PF11_0181, MAL8P1.125), the PheRS (PFA0480w), ThrRS (PF11_0270), LysRS (PF13_0262), MetRS (PF10_0340) and TrpRS (PF13_0205). The program Align2D (sequence alignment module in Modeller) was used to perform dynamic programming-based global alignments of the target and template sequences. This program uses variable gap penalty for structural loops and core regions using information derived from template structures. We found key differences in the conserved motifs in various aaRS s. For example, the class I motif 'KYSKS' in P. falciparum TyrRS (PF11_0181) and 'KMSKS' in MAL8P1.125 differs from 'KLGKS' of human mitochondrial TyrRS (2PID) and 'KMSSS' of human cytoplasmic (1N3L) respectively. Similarly, class I motif 'HIGH' has subtle sequences variations between P. falciparum and H. sapiens TyrRSs (Figure 7a, Table 3). Using the above procedures, we could generate structural models for several Pf-aaRSs. Stereo-chemical qualities of the generated protein models were assessed using PROCHECK (85-90% residues are in allowed regions of Ramachandran plot). The overall superimposed three-dimension models were visualized in CHIMERA and PYMOL (Figure 7b). Many sequence insertions were observed for P. falciparum enzymes when compared to their homologous [63]. Location of insertions in P. falciparum TyrRS between well-conserved secondary structures suggests ability of TyrRS anticodon binding core to accommodate larger sequence inserts with minimum disruption to the catalytic domain. Direct comparison of modeled P. falciparum aaRS s with human aaRS s revealed several other important structural differences. For example, numerous insertions are present in the loop regions linking various α-helices (α10 to α13) in anticodon binding domain of P. falciparum TyrRSs (PF11_0181 and MAL8p1.125) when compared to its human homologous (2PID and 1N3L) respectively. Structural differences between TyrRS (from P. falciparum) and human counterparts are summarized in Table 3 and shown in Figure 7c. These subtle structural changes that manifest as partial conservation of important motifs in P. falciparum aaRSs reflect evolutionary divergence, and may be useful for exploitation of parasite-specific features as drug targets.

Figure 7
figure 7

Left and right panels of the figure represent sequence and structural comparison of bacterial type Plasmodium TyrRS (PF11_0181) with human mitochondrial TyrRS (2PID) and the cytosolic Plasmodium TyrRS (Mal8p1.125) with human cytosolic TyrRS (1N3L). a) A structure-based sequence alignment of the catalytic domain of Plasmodium TyrRSs with human TyrRSs. Insertions in Pf and human sequences are colored in light blue and orange respectively. Class I synthetase conserved motifs are colored red. Residues involved in tRNA recognition and catalysis are indicated in green (same residues in Pf and Hs) and violet & boxed (different in Pf and Hs). The secondary structural elements are shown above the sequence alignments. Conserved residues are indicated by asterisk below the sequence alignment. (b) Superposition of Pf-TyrRS and Hs-TyrRS depicting the structural differences. Pf-Tyr is colored grey and Hs-TyrRS is colored tan. Insertions in Pf-TyrRSs are highlighted in blue whereas Hs-TyrRS insertions are in orange. Motif 1 in Pf (PF11_0181 - HLGN and Mal8p1.125 - HIAQ) and Hs (2PID - HVGH and 1N3L - HVAY) TyrRSs has been encircled red whereas Motif 2 in Pf (PF11_0181 - KLGKS and Mal8p1.125 - KMSKS) and Hs (2PID - KYSKS and 1N3L - KMSSS) is encircled green. (c) Snapshot of the active sites of Pf and Hs TyrRS s (superimposed) structures. Non-conserved active site residues colored violet are encircled.

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Table 3 Structural differences between tyrosyl-tRNA synthetases from human & P. falciparum
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Conclusion

Aminoacyl-tRNA synthetases (aaRS s) link RNA with protein translation. Besides their key role in protein synthesis, aaRS s are also integral to various other cellular processes. aaRS enzymes have been the focus for antimicrobial drug discovery [64, 65]. An example of clinical application of an aaRS inhibitor is provided by the antibiotic mupirocin (marketed as Bactroban), which selectively inactivates bacterial isoleucyl-tRNA synthetase [66]. Similarly, it has been shown that the broad-spectrum antifungal 5-fluoro-1,3-dihydro-1-hydroxy-2,1-benzoxaborole (AN2690) inhibits yeast cytoplasmic leucyl-tRNA synthetase by blocking editing site of the enzyme [67, 68]. Therefore, presence of distinct or tinkered P. falciparum aaRS lends an opportunity for their exploitation as new drug targets against malaria. In this study, we have extensively analyzed aaRS sequences from Plasmodium species in terms of their mRNA/protein expression profiles, their cellular localization, their organelle targeting and their unique sequence/domain attributes. We have discovered several distinct aaRS s in P. falciparum with no clear human counterparts in terms of their overall domain structures. We have also highlighted deviations of some highly conserved sequence motifs and active site sequence clusters. Our analyses clearly show that a larger fraction of P. falciparum proteome is devoted to aaRS when compared with many other organisms. The phylogenetic data hint at evolutionary closeness of some Pf-aaRSs to bacteria and plants - this further supports the fact of secondary endosymbiosis in this apicomplexan. We hope that our in-depth phylogenetic, protein targeting, domain architecture, protein expression profiling and homology modeling data on Pf-aaRSs can be used as a platform for experimental studies of this important protein family in malaria parasites.

Methods

Sequence extraction

The P. falciparum genome database PlasmoDB Release 5.4 was used for the present analyses. Sequence sets of all the aaRS s from other organisms includes P. berghei, P. chabaudi, P. falciparum, P. knowlesi, P. yoelii, P. vivax, H. sapiens, M. tuberculosis, D. discoidium, M. jannaschii, R. norvegicus, C. parvum, B. bovis, S. cerevisiae, D. melanogaster, Y. pestis, T. aquaticus, S. pneumoniae, S. entrica, E. coli, A. thaliana, A. pisum, A. salmonicida, B. cereus, B. thuringiensis, B. afzelii, B. burgdorferi, B. garinii, B. valaisiana, Bradyrhizobium, B. pennsylvanicus, C. acidaminovorans, H. defensa, C. taiwanensis, E. fergusonii, F. bacterium, F. novicida, F. tularensis, F. alni, G. tenuistipitata, H. arsenicoxydans, A. cellulolyticus, A. chlorophenolicus, A. ferrooxidans, Algoriphagus, A. muciniphila, Anaeromyxobacter, A. thermophilum, B. ambifaria, B. indica, B. mycoides, B. taurus, B. tribocorum, C. atlanticus, Caulobacter, C. aurantiacus, C. cellulolyticum, Citrobacter, C. pinensis, C. Ruthia, Cyanothece, D. desulfuricans, D. hafniense, Diaphorobacter, D. shibae, D. turgidum, E. cuniculi, E. lenta, E. ruminantium, Exiguobacterium, G. diazotrophicus, Geobacillus, M. maris, N. multipartita, Nocardioides, O. terrae, P. abelii, P. atlantica, P. denitrificans, P. ingrahamii, P. lavamentivorans, R. castenholzii, S. arenicola, S. fumaroxidans, X. autotrophicus, V. vadensis, V. paradoxus, T. whipplei, T. auensis, S. stellata, Ch. parvum, S. heliotrinireducens, Silicibacter, S. putrefaciens, S. usitatus, Thauera, X. laevis, Theileria annulata, Vibrio fischeri, W. succinogenes, X. tropicalis, Zeamays. Additional sequences were obtained based on sequence similarity via NCBI BLAST [69] and ENSEMBL [70] databases. Known sequence motifs of aaRS s have been used as templates to retrieve sequences of aaRS from other organisms. Some aaRS sequences were manually annotated based on the presence of signature motifs. Protein domains and motifs in the predicted aaRS s were identified using following programs - Superfamily [71], SMART [72] and MotifScan available at expasy web server. The following databases - Pfam [73], TIGR, PIR, EBI and PlasmoDB were also extensively used. Hidden Markov Model (HMM) for each of the 20 aaRS were constructed by the software package Sequence Alignment and Modeling System version 2.2.1 (SAM) [74] exploiting sequences in the aaRS database [75]. HMM profiles were then used to carry out database search vs P. falciparum proteins. A score was assigned to each protein by calculating the probability that the corresponding sequence is generated by the HMM model, hence for each database search a score distribution was obtained. The score distributions were normalized and 4 ranges of values were considered to identify aaRS (c > 5, 10 < c < 20, 20 < c < 50, c < 50).

Expression and Localization

The prediction of signal sequences for cellular localization in P. falciparum was performed using various available online web-servers - MITOPROT [76], PredictNLS [77] and PATS [78] for mitochondria, nucleus and apicoplast respectively. PEXEL motif prediction was been carried out by querying PlasmoDB. To identify specific gene expression profiles, we have combined information from different data sets. For the spotted oligonucleotide array data, only half of the 48 time points of the intra-erythrocytic cycle are shown for simplicity, and ratios (versus a common reference) were log2-transformed prior to cluster analysis. For the photolithography data, CEL files were downloaded from website and transferred into Bioconductor package for analysis using a robust multi-array averaging algorithm (RMA) for background adjustment and quantiles normalization [79]. Genes whose expression level was less than 10 (too close to background) or the logP was greater than -0.5 (too few probes per gene) were removed from dataset. Total intensity values for each time point were converted to mean-centered ratios by dividing the total intensity by the average intensity for that gene across all experimental conditions and were then log2-transformed prior to clustering. These data manipulations were necessary because the oligo-nucleotide array data was collected as the intensity ratio between the experimental sample and a common reference, while the photolithography data was collected as the total signal intensity at each spot. Gene expression patterns where the minimum percentage of existing values was less than 80% were eliminated from rest of the analysis. The remaining missing values were replaced by using the KNN-imputation method [80].

Phylogenetic analysis

To explore the evolutionary relationships amongst aaRSs phylogenetic analyses were performed for each P. falciparum aaRS on an expanded set of 102 sequences. Multiple sequence alignments of these sequences were obtained from CLUSTALW with default parameters (performed locally) in PHYLIP format [81]. These MSAs were used as seed sequences to run PHYML_v2.4.4 using Jones-Taylor-Thornton (JTT) model [82]. The resulting file was further used in MEGA4.2 for visualization of trees [83].

Model Building and Validation

We used Sali's Modeller8v2 [84] tool for building various P. falciparum aaRS s models. The stereo-chemical quality of modeled proteins was verified by PROCHECK [85]. Structural mapping of active site residues and other motifs was performed using CHIMERA [86] and PYMOL [87].

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Acknowledgements

TKB, CK and AS are supported by grants from the Department of Biotechnology, Govt. Of India. SK is supported by MEPHITIS grant. This work has been conducted as part of MEPHITIS project and partially funded by the European Commission (Grant Agreement no: HEALTH-F3-2009-223024).

Author information

Authors and Affiliations

  1. Structural and Computational Biology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110 067, India

    Tarun Kumar Bhatt, Charu Kapil, Sameena Khan & Amit Sharma

  2. Department of Biosciences, Jamia Millia Islamia University, Jamia Nagar, New-Delhi, 110 025, India

    Charu Kapil & Mohamad Aman Jairajpuri

  3. Department of Bioscience and Biotechnology, Banasthali Vidyapith University, Banasthali, Rajasthan, 304 022, India

    Sameena Khan & Vinay Sharma

  4. Barcelona Institute for Research in Biomedicine, Barcelona Science Park, C/Samitier 1-5, Barcelona, 08015, Catalonia, Spain

    Daniele Santoni

  5. Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy

    Daniele Santoni, Francesco Silvestrini & Elisabetta Pizzi

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  1. Tarun Kumar Bhatt
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Correspondence to Amit Sharma.

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Authors' contributions

TKB, CK and SK carried out the computational experiments and data analysis and wrote the paper; MAJ and VS contributed to the manuscript writing; DS and EP carried out HMM construction and database search by HMM; FS performed analysis of transcriptomic and proteomic data; AS designed the study and supervised the work. All authors have read and approved the final manuscript.

Electronic supplementary material

12864_2009_7087_MOESM1_ESM.PDF

Additional file 1:List of Pf-aaRSs categorized into class I, class II, and related proteins. Gene ID, gene location, description of product and its length are given. (PDF 44 KB)

12864_2009_7087_MOESM2_ESM.PDF

Additional file 2:Phylogenetic trees of aaRSs from P. falciparum. The evolutionary tree was constructed by the method PHYML using the MEGA 4.0. P. falciparum aaRSs are labeled green triangles. 102 species considered for the evolutionary analysis are taken from the three domains of life viz. P. berghei, P. chabaudi, P. falciparum, P. knowlesi, P. yoelii, P. vivax, H. sapiens, M. tuberculosis, D. discoidium, M. jannaschii, R. norvegicus, C. parvum, B. bovis, S. cerevisiae, D. melanogaster, Y. pestis, T. aquaticus, S. pneumoniae, S. entrica, E. coli, A. thaliana, A. pisum, A. salmonicida, B. cereus, B. thuringiensis, B. afzelii, B. burgdorferi, B. garinii, B. valaisiana, Bradyrhizobium, B. pennsylvanicus, C. acidaminovorans, H. defensa, C. taiwanensis, E. fergusonii, F. bacterium, F. novicida, F. tularensis, F. alni, G. tenuistipitata, H. arsenicoxydans, A. cellulolyticus, A. chlorophenolicus, A. ferrooxidans, Algoriphagus, A. muciniphila, Anaeromyxobacter, A. thermophilum, B. ambifaria, B. indica, B. mycoides, B. taurus, B. tribocorum, C. atlanticus, Caulobacter, C. aurantiacus, C. cellulolyticum, Citrobacter, C. pinensis, C. Ruthia, Cyanothece, D. desulfuricans, D. hafniense, Diaphorobacter, D. shibae, D. turgidum, E. cuniculi, E. lenta, E. ruminantium, Exiguobacterium, G. diazotrophicus, Geobacillus, M. maris, N. multipartita, Nocardioides, O. terrae, P. abelii, P. atlantica, P. denitrificans, P. ingrahamii, P. lavamentivorans, R. castenholzii, S. arenicola, S. fumaroxidans, X. autotrophicus, V. vadensis, V. paradoxus, T. whipplei, T. auensis, S. stellata, Ch. parvum, S. heliotrinireducens, Silicibacter, S. putrefaciens, S. usitatus, Thauera, X. laevis, Theileria annulata, Vibrio fischeri, W. succinogenes, X. tropicalis, Zeamays. (PDF 5 MB)

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Bhatt, T.K., Kapil, C., Khan, S. et al. A genomic glimpse of aminoacyl-tRNA synthetases in malaria parasite Plasmodium falciparum. BMC Genomics 10, 644 (2009). https://doi.org/10.1186/1471-2164-10-644

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BMC Genomics

ISSN: 1471-2164

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