Journal of Lipid Research
MethodsMorphology and structure of lipoproteins revealed by an optimized negative-staining protocol of electron microscopy[S]
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- apo
apolipoprotein
- CE
cholesteryl ester
- cryoEM
cryo-electron microscopy
- cryo-NS
cryo-negative-staining
- DPBS
Dulbecco's phosphate-buffered saline
- EM
electron microscopy
- IDL
intermediate density lipoprotein
- NS
negative staining
- NS-EM
negative-staining electron microscopy
- PTA
phosphotungstic acid
- RCT
reverse cholesterol transport
- rHDL
reconstituted HDL
- UC
unesterified cholesterol
- UF
uranyl formate
Abbreviations:
This work was supported by the Office of Science, Office of Basic Energy Sciences of the U.S. Department of Energy (contract no. DE-AC02-05CH11231) and the W. M. Keck foundations to G.R. L.Z. was supported in part by the State Scholarship of China through China Scholarship Council (file no. 2008628018).
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L. Zhang and J. Song contributed equally to this work.
- [S]
The online version of this article (available at http://www.jlr.org) contains supplementary data in the form of two figures.