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Identification of Differentially Expressed MicroRNA in Parathyroid Tumors

  • Translational Research and Biomarkers
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Annals of Surgical Oncology Aims and scope Submit manuscript

Abstract

Background

The molecular factors that control parathyroid tumorigenesis are poorly understood. In the absence of local invasion or metastasis, distinguishing benign from malignant parathyroid neoplasm is difficult on histologic examination. We studied the microRNA (miRNA) profile in normal, hyperplastic, and benign and malignant parathyroid tumors to better understand the molecular factors that may play a role in parathyroid tumorigenesis and that may serve as diagnostic markers for parathyroid carcinoma.

Methods

miRNA arrays containing 825 human microRNAs with four duplicate probes per miRNA were used to profile parathyroid tumor (12 adenomas, 9 carcinomas, and 15 hyperplastic) samples normalized to four reference normal parathyroid glands. Differentially expressed miRNA were validated by real-time quantitative TaqMan polymerase chain reaction (PCR).

Results

One hundred fifty-six miRNAs in parathyroid hyperplasia, 277 microRNAs in parathyroid adenoma, and 167 microRNAs in parathyroid carcinomas were significantly dysregulated as compared with normal parathyroid glands [false discovery rate (FDR) < 0.05]. By supervised clustering analysis, all parathyroid carcinomas clustered together. Three miRNAs (miR-26b, miR-30b, and miR-126*) were significantly dysregulated between parathyroid carcinoma and parathyroid adenoma. Receiver-operating characteristic curve analysis showed mir-126* was the best diagnostic marker, with area under the curve of 0.776.

Conclusions

Most miRNAs are downregulated in parathyroid carcinoma, while in parathyroid hyperplasia most miRNAs are upregulated. miRNA profiling shows distinct differentially expressed miRNAs by tumor type which may serve as helpful adjunct to distinguish parathyroid adenoma from carcinoma.

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Acknowledgment

This work was supported in part by grants from the UCSF Comprehensive Cancer Center, American Cancer Society, and the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research.

Conflicts of interest

None.

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Authors and Affiliations

  1. Endocrine Oncology Section, Surgery Branch, National Cancer Institute, Bethesda, MD, USA

    Reza Rahbari MD, Mei He MD & Electron Kebebew MD

  2. Gladstone Institute, University of California San Francisco, San Francisco, CA, USA

    Alisha K. Holloway PhD

  3. Department of Pathology, University of California San Francisco, San Francisco, CA, USA

    Elham Khanafshar MD

  4. Department of Surgery, University of California San Francisco, San Francisco, CA, USA

    Orlo H. Clark MD

Authors
  1. Reza Rahbari MD
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  2. Alisha K. Holloway PhD
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  3. Mei He MD
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  4. Elham Khanafshar MD
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  5. Orlo H. Clark MD
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  6. Electron Kebebew MD
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Correspondence to Electron Kebebew MD.

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Rahbari, R., Holloway, A.K., He, M. et al. Identification of Differentially Expressed MicroRNA in Parathyroid Tumors. Ann Surg Oncol 18, 1158–1165 (2011). https://doi.org/10.1245/s10434-010-1359-7

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  • DOI: https://doi.org/10.1245/s10434-010-1359-7

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