Endothelial Cells Constituting Blood-nerve Barrier Have Highly Specialized Characteristics as Barrier-forming Cells

Yasuteru Sano; Fumitaka Shimizu; Hiroto Nakayama; Masaaki Abe; Toshihiko Maeda; Sumio Ohtsuki; Tetsuya Terasaki; Masuo Obinata; Masatsugu Ueda; Ri-ichi Takahashi; Takashi Kanda

doi:10.1247/csf.07015

Yasuteru Sano, Fumitaka Shimizu, Hiroto Nakayama, Masaaki Abe, Toshihiko Maeda, Sumio Ohtsuki, Tetsuya Terasaki, Masuo Obinata, Masatsugu Ueda, Ri-ichi Takahashi, Takashi Kanda

Author information

Yasuteru Sano
Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
Fumitaka Shimizu
Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
Hiroto Nakayama
Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
Masaaki Abe
Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
Toshihiko Maeda
Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
Sumio Ohtsuki
Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University
Tetsuya Terasaki
Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University
Masuo Obinata
Department of Cell Biology, Institute of Development, Aging and Cancer, Tohoku University
Masatsugu Ueda
The YS Institute, Inc.
Ri-ichi Takahashi
The YS Institute, Inc.
Takashi Kanda
Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine

Keywords: blood-nerve barrier, peripheral nerve microvascular endothelial cells, tight junction, claudin-5, transporter

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2007 Volume 32 Issue 2 Pages 139-147

DOI https://doi.org/10.1247/csf.07015

View "Advance Publication" version (December 5, 2007).

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Abstract

In autoimmune disorders of the peripheral nervous system (PNS) such as Guillain-Barré syndrome and chronic inflammatory demyelinating polyradiculoneuropathy, breakdown of the blood-nerve barrier (BNB) has been considered as a key step in the disease process. Hence, it is important to know the cellular property of peripheral nerve microvascular endothelial cells (PnMECs) constituting the bulk of BNB. Although many in vitro models of the blood-brain barrier (BBB) have been established, very few in vitro BNB models have been reported so far. We isolated PnMECs from transgenic rats harboring the temperature-sensitive SV40 large T-antigen gene (tsA58 rat) and investigated the properties of these “barrier-forming cells”. Isolated PnMECs (TR-BNBs) showed high transendothelial electrical resistance and expressed tight junction components and various types of influx as well as efflux transporters that have been reported to function at BBB. Furthermore, we confirmed the in vivo expression of various BBB-forming endothelial cell markers in the endoneurium of a rat sciatic nerve. These results suggest that PnMECs constituting the bulk of BNB have a highly specialized characteristic resembling the endothelial cells forming BBB.

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