Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Regular Articles
Effect of Sodium Diclofenac on Serum and Tissue Concentration of Amoxicillin and on Staphylococcal Infection
Francisco Carlos GroppoRoberta Pessoa SimõesJuliana Cama RamacciatoVera RehderEduardo Dias de AndradeThales Rocha Mattos-Filho
Author information
JOURNAL FREE ACCESS

2004 Volume 27 Issue 1 Pages 52-55

Details
Abstract

The effect of sodium diclofenac on serum and tissue amoxicillin concentration as well as their effect against staphylococcal infection was observed. Four polyurethane sponges were placed in the back of thirty rats. After 14 d, two granulomatous tissues received 0.5 ml of 108 cfu/ml (Staphylococcus aureus). Two days later, the rats were divided into five groups: group 1 received amoxicillin 50 mg/kg/p.o., group 2 received amoxicillin 25 mg/kg/p.o., group 3 received sodium diclofenac 2.5 mg/kg/i.m. and amoxicillin 50 mg/kg/p.o., group 4 received sodium diclofenac 2.5 mg/kg/i.m., and group 5 (control group) received NaCl 1 ml/p.o. After six hours of drug administration, blood serum (10 μl) and noninfected granulomatous tissues were placed on Mueller–Hinton agar inoculated with 108 cfu/ml (S. aureus). Infected tissues were dispersed in a sonic system and were spread (10 μl) on salt mannitol agar. Microorganisms were counted and the inhibition zones were measured after 18 h of incubation at 37 °C. Amoxicillin tissue concentration was 6.27 μg/g for group 1, 2.18 μg/g for group 2, and 0.72 μg/g for group 3. The serum concentrations were 11.56 μg/ml for group 1, 5.36 μg/ml for group 2, and 1.34 μg/ml for group 3. No differences were observed among group 1, 2, and 3 regarding staphylococci counts (Kruskall–Wallis test p>0.05). Group 4 reduced (p<0.05) staphylococci counts comparing to group 5. It was concluded that sodium diclofenac reduced serum and tissue amoxicillin concentration and, even in large doses, amoxicillin was not effective in eradicating the staphylococcal infection after 6 h of administration.

Content from these authors
© 2004 The Pharmaceutical Society of Japan
Previous article Next article
feedback
Top