Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
Determination of Barbaloin in Rat Serum
YASUKO ISHIIHISAYUKI TANIZAWAYOSHIO TAKINO
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1987 Volume 35 Issue 11 Pages 4642-4644

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Abstract

A procedure for assay of barbaloin by thin layer chromatography (TLC) -densitometry was developed. The method is based on measuring the yellow-green fluorescence, which is produced by the reaction of barbaloin and borax (Schoutelen's reaction), on the TLC plate with a fluorophotometer. The limit of detection was about 0.01 μg of barbaloin, corresponding to about 0.033 μg/ml of barbaloin in rat serum. This method was applied for the determination of barbaloin in serum after oral administration of barbaloin (100 mg/kg) to rats. The serum concentration of barbaloin was extremely low; the peak concentration was only 0.34 μg/ml.

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© The Pharmaceutical Society of Japan
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