Evaluation of pretreatment with Pleurotus ostreatus for enzymatic hydrolysis of rice straw

doi:10.1263/jbb.100.637

Journal of Bioscience and Bioengineering

Volume 100, Issue 6, December 2005, Pages 637-643

https://doi.org/10.1263/jbb.100.637 Get rights and content

The effects of biological pretreatment of rice straw using four white-rot fungi (Phanerochaete chrysosporium, Trametes versicolor, Ceriporiopsis subvermispora, and Pleurotus ostreatus) were evaluated on the basis of quantitative and structural changes in the components of the pretreated rice straw as well as susceptibility to enzymatic hydrolysis. Of these white-rot fungi, P. ostreatus selectively degraded the lignin fraction of rice straw rather than the holocellulose component. When rice straw (water content of 60%) was pretreated with P. ostreatus for 60 d, the total weight loss and the degree of Klason lignin degraded were 25% and 41%, respectively. After the pretreatment, the residual amounts of cellulose and hemicellulose were 83% and 52% of those in untreated rice straw, respectively. By enzymatic hydrolysis with a commercial cellulase preparation for 48 h, 52% holocellulose and 44% cellulose in the pretreated rice straw were solubilized. The net sugar yields based on the amounts of holocellulose and cellulose of untreated rice straw were 33% for total soluble sugar from holocellulose and 32% for glucose from cellulose. The SEM observations showed that the increase in susceptibility of rice straw to enzymatic hydrolysis by pretreatment with P. ostreatus is caused by partial degradation of the lignin seal. When the content of Klason lignin was less than 15% of the total weight of the pretreated straw, enhanced degrees of enzymatic solubilization of holocellulose and cellulose fractions were observed as the content of Klason lignin decreased.

Section snippets

Microorganisms and preparation of inoculum

Phanerochaete chrysosporium NBRC (IFO)31249, Trametes (Coriolus) versicolor NBRC (IFO) 4937, Ceriporiopsis subvermispora ATCC 90467, and Pleurotus ostreatus ATCC 66376 were used as white-rot fungi for biological pretreatment. Mycelial mats of each white-rot fungus, which have been grown on 3.9% potato dextrose agar (Nissui Seiyaku, Tokyo) slants in test tubes, were suspended in a solution of 0.1% Tween 80 (Wako Pure Chemical Industries, Osaka) and then inoculated on solid medium containing 8 g

Selection of basidiomycetes

For the pretreatment, cultures of white-rot fungi were prepared using moistened wheat bran as the solid substrate. P. chrysosporium grew faster than the other fungi and about 200 mg/g dry matter was obtained at 3–5 d (data not shown). The cell mass of T. versicolor reached 450–500 mg/g dry matter at 10 d (data not shown). The growth rates of C. subvermispora and P. ostreatus were relatively slow, but the cell mass obtained was high, 500–550 mg/g dry matter for C. subvermispora and about 350

Acknowledgments

This work was supported in part by a Grant-in-Aid from Akita Prefectural Organization for Academic Cooperation. The authors are grateful to Mr. Hiroshi Saito for the observation by scanning electron microscopy.

References (26)

A. Guerra et al.
Molecular weight distribution of wood components extracted from Pinus taeda biotreated by Ceriporiopsis subvermispora
Enzyme Microb. Technol.
(2003)
H. Itoh et al.
Bioorganosolve pretreatment for simultaneous saccharification and fermentation of beech wood by ethanolysis and white rot fungi
J. Biotechnol.
(2003)
T. Yoshida et al.
Gasification of biomass model compounds and real biomass in supercritical water
Biomass Bioenergy
(2004)
K. Okano et al.
Conversion of Japanese red cedar (Cryptomeria japonica) into a feed for ruminants by white-rot basidiomycetes
Animal Feed. Sci. Technol.
(2005)
M. Tien et al.
Lignin peroxidase of Phanerochaete chrysosporium
P.S. Chahal et al.
Lignocellulose waste: biological conversion
C.S. Gong et al.
Ethanol production from renewable resources
Y. Nakamura et al.
Enhanced ethanol production from enzymatic treated steam exploded rice straw using extractive fermentation
J. Chem. Technol. Biotechnol.
(2001)
B. Yang et al.
Fast and efficient alkaline peroxide treatment to enhance the enzymatic digestibility of steam-exploded softwood substrate
Biotechnol. Bioeng.
(2002)
B. Yang et al.
Effect of xylan and lignin removal by batch and flowthrough pretreatment on the enzymatic digestibility of corn stover cellulose
Biotechnol. Bioeng.
(2004)

A. Wingren et al.

Process consideration and economic evaluation of two-step steam pretreatment for production of fuel ethanol from softwood

Biotechnol. Prog.

(2004)

X. Pan et al.

Biorefining of softwoods using ethanol organosolv pulping: preliminary evaluation of process streams for manufacture of fuel-grade ethanol and co-products

Biotechnol. Bioeng.

(2005)

A.I. Hatakka

Pretreatment of wheat straw by white-rot fungi for enzymic saccharification of cellulose

Eur. J. Appl. Microbiol. Biotechnol.

(1983)

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Journal of Bioscience and Bioengineering

Section snippets

Microorganisms and preparation of inoculum

Selection of basidiomycetes

Acknowledgments

References (26)

Molecular weight distribution of wood components extracted from Pinus taeda biotreated by Ceriporiopsis subvermispora

Enzyme Microb. Technol.

Bioorganosolve pretreatment for simultaneous saccharification and fermentation of beech wood by ethanolysis and white rot fungi

J. Biotechnol.

Gasification of biomass model compounds and real biomass in supercritical water

Biomass Bioenergy

Conversion of Japanese red cedar (Cryptomeria japonica) into a feed for ruminants by white-rot basidiomycetes

Animal Feed. Sci. Technol.

Lignin peroxidase of Phanerochaete chrysosporium

Lignocellulose waste: biological conversion

Ethanol production from renewable resources

Enhanced ethanol production from enzymatic treated steam exploded rice straw using extractive fermentation

J. Chem. Technol. Biotechnol.

Fast and efficient alkaline peroxide treatment to enhance the enzymatic digestibility of steam-exploded softwood substrate

Biotechnol. Bioeng.

Effect of xylan and lignin removal by batch and flowthrough pretreatment on the enzymatic digestibility of corn stover cellulose

Biotechnol. Bioeng.

Process consideration and economic evaluation of two-step steam pretreatment for production of fuel ethanol from softwood

Biotechnol. Prog.

Biorefining of softwoods using ethanol organosolv pulping: preliminary evaluation of process streams for manufacture of fuel-grade ethanol and co-products

Biotechnol. Bioeng.

Pretreatment of wheat straw by white-rot fungi for enzymic saccharification of cellulose

Eur. J. Appl. Microbiol. Biotechnol.

Cited by (355)

Microbial pretreatment of camelina straw and switchgrass by Trametes versicolor and Phanerochaete chrysosporium to improve physical quality and enhance enzymatic digestibility of solid biofuel pellets

Enhancement of methane production from livestock manure with pre-treatments based in fungi of genus Pleurotus

A thermo-chemical and biotechnological approaches for bamboo waste recycling and conversion to value added product: Towards a zero-waste biorefinery and circular bioeconomy

Prospects of rice straw for sustainable production of biofuels: Current trends and challenges

Improving enzymatic hydrolysis of lignocellulosic biomass by bio-coordinated physicochemical pretreatment—A review

Sugar production from husk coffee using combined pretreatments