Evaluation of pretreatment with Pleurotus ostreatus for enzymatic hydrolysis of rice straw
Section snippets
Microorganisms and preparation of inoculum
Phanerochaete chrysosporium NBRC (IFO)31249, Trametes (Coriolus) versicolor NBRC (IFO) 4937, Ceriporiopsis subvermispora ATCC 90467, and Pleurotus ostreatus ATCC 66376 were used as white-rot fungi for biological pretreatment. Mycelial mats of each white-rot fungus, which have been grown on 3.9% potato dextrose agar (Nissui Seiyaku, Tokyo) slants in test tubes, were suspended in a solution of 0.1% Tween 80 (Wako Pure Chemical Industries, Osaka) and then inoculated on solid medium containing 8 g
Selection of basidiomycetes
For the pretreatment, cultures of white-rot fungi were prepared using moistened wheat bran as the solid substrate. P. chrysosporium grew faster than the other fungi and about 200 mg/g dry matter was obtained at 3–5 d (data not shown). The cell mass of T. versicolor reached 450–500 mg/g dry matter at 10 d (data not shown). The growth rates of C. subvermispora and P. ostreatus were relatively slow, but the cell mass obtained was high, 500–550 mg/g dry matter for C. subvermispora and about 350
Acknowledgments
This work was supported in part by a Grant-in-Aid from Akita Prefectural Organization for Academic Cooperation. The authors are grateful to Mr. Hiroshi Saito for the observation by scanning electron microscopy.
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