Abstract
We introduce a direct wavefront sensing method using structures labeled with fluorescent proteins in tissues as guide stars. An adaptive optics confocal microscope using this method is demonstrated for imaging of mouse brain tissue. A dendrite and a cell body of a neuron labeled with yellow fluorescent protein are tested as guide stars without injection of other fluorescent labels. Photobleaching effects are also analyzed. The results shows increased image contrast and improvement in the signal intensity for fixed mouse tissues at depths of .
© 2011 Optical Society of America
Full Article | PDF ArticleMore Like This
Xiaodong Tao, Bautista Fernandez, Oscar Azucena, Min Fu, Denise Garcia, Yi Zuo, Diana C. Chen, and Joel Kubby
Opt. Lett. 36(7) 1062-1064 (2011)
Xiaodong Tao, Justin Crest, Shaila Kotadia, Oscar Azucena, Diana C. Chen, William Sullivan, and Joel Kubby
Opt. Express 20(14) 15969-15982 (2012)
Antoine Hubert, Fabrice Harms, Rémy Juvénal, Pauline Treimany, Xavier Levecq, Vincent Loriette, Georges Farkouh, François Rouyer, and Alexandra Fragola
Opt. Lett. 44(10) 2514-2517 (2019)