Chest
Original ResearchCOPDProteomic Analysis in Lung Tissue of Smokers and COPD Patients
Section snippets
Study Subjects
Nontumorous lung tissue specimens were obtained from patients who underwent lobectomy or pneumonectomy for lung cancer. After surgical resection, these lung tissues were immediately frozen to − 80°C; they were then donated and stored at the Korea Lung Tissue Bank, which has been assigned and supported by the Korean Science and Engineering Foundation in the Ministry of Sciences and Technology. All patients included in the study gave their consent, and the study protocol was approved by the
Two-Dimensional Polyacrylamide Gel Electrophoresis
The COPD group revealed a mean age of 56.0 years (mean tobacco smoking history, 38.3 pack-years). Mean FEV1 was 66.1% of predicted in the COPD patient group. Similarly, mean age in the nonsmoker group was 59.0 years, and mean age and mean pack-years of the healthy smoker group were 64.5 years and 48.7 pack-years, respectively. Age, sex, smoking history, and pulmonary function data of patients are summarized in Table 1.
Figure 1A shows an example of the increased expression in COPD patients as
Discussion
In this study, we have shown that the expressions of MMP-13 and thioredoxin-like 2 were increased in the COPD lung tissues. MMP-13 was mainly expressed in the alveolar macrophages and type II pneumocytes. In contrast, thioredoxin-like 2 was primarily seen in the bronchial epithelium.
MMP-13, one of the proteolytic enzymes, is thought to be mainly expressed by chondrocytes and synovial cells in human osteoarthritis and rheumatoid arthritis; it has a role in cartilage destruction. MMP-13 also is
References (32)
- et al.
COPD: the dangerous underestimate of 15%
Lancet
(2006) - et al.
An overview of collagenase-3 expression in malignant tumors and analysis of its potential value as a target in antitumor therapies
Clin Chim Acta
(2000) - et al.
Cell type-specific effect of hypoxia and platelet-derived growth factor-BB on extracellular matrix turnover and its consequences for lung remodeling
J Biol Chem
(2007) - et al.
Upregulation of gelatinases A and B, collagenases 1 and 2, and increased parenchymal cell death in COPD
Chest
(2000) - et al.
Increased activity of matrix metalloproteinase-8 and matrix metalloproteinase-9 in induced sputum from patients with COPD
Chest
(2004) - et al.
Characterization of human thioredoxin-like 2: a novel microtubule-binding thioredoxin expressed predominantly in the cilia of lung airway epithelium and spermatid manchette and axoneme
J Biol Chem
(2003) - et al.
Redox modulation of chromatin remodeling: impact on histone acetylation and deacetylation, NF-κB and pro-inflammatory gene expression
Biochem Pharmacol
(2004) - et al.
Elevated serum levels of thioredoxin in patients with acute exacerbation of asthma
Immunol Lett
(2003) - et al.
Proteomic analysis of peripheral T-lymphocytes in patients with asthma
Chest
(2007) - et al.
The natural history of chronic bronchitis and emphysema
(1976)
Use of proteomic patterns of serum biomarkers in patients with chronic obstructive pulmonary disease: correlation with clinical parameters
Proc Am Thorac Soc
Proteomic study of human bronchoalveolar lavage fluids from smokers with chronic obstructive pulmonary disease by combining surface-enhanced laser desorption/ionization-mass spectrometry profiling with mass spectrometric protein identification
Proteomics
Protein expression in sputum of smokers and chronic obstructive pulmonary disease patients: a pilot study by CapLC-ESI-Q-TOF
J Proteome Res
Bronchoalveolar lavage fluid protein composition in patients with sarcoidosis and idiopathic pulmonary fibrosis: a two-dimensional electrophoretic study
Electrophoresis
Human bronchoalveolar lavage fluid protein two-dimensional database: study of interstitial lung diseases
Electrophoresis
Susceptibility to oxidative stress: proteomic analysis of bronchoalveolar lavage from ozone-sensitive and ozone-resistant strains of mice
Proteomics
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This work was supported by grant R21-2007-000-10058-0 from the National Research Resource Bank Program.
This work was performed at the Division of Respiratory and Critical Care Medicine, Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea.
The authors have no conflicts of interest to disclose.
Reproduction of this article is prohibited without written permission from the American College of Chest Physicians (www.chestjournal.org/misc/reprints.shtml).