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Bioconversion of cellulose into ethanol by nonisothermal simultaneous saccharification and fermentation

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Abstract

The kinetic characteristics of cellulase and β-glucosidase during hydrolysis were determined. The kinetic parameters were found to reproduce experimental data satisfactorily and could be used in a simultaneous saccharification and fermentation (SSF) system by coupling with a fermentation model. The effects of temperature on yeast growth and ethanol production were investigated in batch cultures. In the range of 35–45°C, using a mathematical model and a computer simulation package, the kinetic parameters at each temperature were estimated. The appropriate forms of the model equation for the SSF considering the effects of temperature were developed, and the temperature profile for maximizing the ethanol production was also obtained. Briefly, the optimum temperature profile began at a low temperature of 35°C, which allows the propagation of cells. Up to 10 h, the operating temperature increased rapidly to 39°C, and then decreased slowly to 36°C. In this nonisothermal SSF system with the above temperature profile, a maximum ethanol production of 14.87 g/L was obtained.

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Abbreviations

k′ 1 :

Specific rate of hydrolysis (h)

k′ 2 :

Lumped constants (g[L·h])

K m :

Michaelis constant of β-glucosidase (g/L)

K p :

Monod saturation constant for ethanol production (g/L)

K G :

Monod saturation constant for cell growth (g/L)

K 1B :

Inhibition constant of cellulase for cellobiose (g/L)

K 1G :

Inhibition constant of cellulase for glucose (g/L)

K 2B :

Inhibition constants of β-glucosidase for cellobiose (g/L)

m :

Maintenance energy coefficient (h)

P :

Ethanol concentration (g/L)

P m :

Ethanol concentration above which cells do not grow (g/L)

P′ m :

Ethanol concentration above which cells do not produce (g/L)

r 1 :

Volumetric rate of cellulose utilization (g[L·h])

r 2 :

Volumetric rate of cellobiose utilization (g[L·h])

r 3 :

Volumetric rate of cellobiose utilization (g[L·h])

X :

Cell concentration (g/L)

λ:

Specific rate of enzyme deactivation (h)

λ1 :

Specific deactivation rate of cellulase (h)

λ2 :

Specific deactivation rate of β-glucosidase (h)

μ m :

Maximum specific growth rate (h)

μ i :

Specific growth rate of cells in the presence of ethanol (h)

μ0 :

Maximum growth rate of cells in the absence of concentration (h)

v i :

Specific rate of ethanol production in the presence of ethanol (h)

v0 :

Maximum rate of ethanol production in the absence of ethanol (h)

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Authors and Affiliations

  1. Department of Industrial Chemistry, Dankook University, 330-714, Cheonan, Korea

    Kyeong-Keun Oh

  2. Department of Chemical Engineering, Korea University, 136-701, Seoul, Korea

    Seung-Wook Kim & Suk-In Hong

  3. Department of Food Science and Technology, Chonbuk National University, 560-756, Chonju, Korea

    Yong-Seob Jeong

Authors
  1. Kyeong-Keun Oh
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  2. Seung-Wook Kim
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  3. Yong-Seob Jeong
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  4. Suk-In Hong
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Correspondence to Suk-In Hong.

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Oh, KK., Kim, SW., Jeong, YS. et al. Bioconversion of cellulose into ethanol by nonisothermal simultaneous saccharification and fermentation. Appl Biochem Biotechnol 89, 15–30 (2000). https://doi.org/10.1385/ABAB:89:1:15

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  • DOI: https://doi.org/10.1385/ABAB:89:1:15

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