Abstract
In the present study, we report the results of the capability of zinc chloride for the induction of micronuclei in cultured human leukocytes using cytokinesis-block micronucleus assay. Two concentrations of zinc chloride (1.5 × 10−4 M and 3.0 × 10−4 M) were used to evaluate the potential of this zinc salt to induce micronucleus formation. This effect was compared with positive (mitomycin C treated) and negative controls (no salt added). Our results show a significant (p ≤ 0.001) increase of micronucleated cytokinesis-blocked cells (MNCBs) in zinc-chloride-treated cells compared to the negative control. Induction of MNCBs was not in a dose-dependent manner for zinc chloride concentrations tested. This report is the first to describe the efficiency of cytokinesis-block micronucleus assay to evaluate the genotoxic effects of zinc salt.
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Santra, M., Das, S.K., Talukder, G. et al. Induction of micronuclei by zinc in human leukocytes. Biol Trace Elem Res 88, 139–144 (2002). https://doi.org/10.1385/BTER:88:2:139
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DOI: https://doi.org/10.1385/BTER:88:2:139