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Neuroendocrine marker expression in thyroid epithelial tumors

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Abstract

Tissue sections from 50 cases with thyroid tumors, composed of 11 follicular adenomas, 10 follicular carcinomas, 14 papillary carcinomas, 10 anaplastic carcinomas, and 5 medullary carcinomas, were immunohistochemically analyzed for representative neuroendocrine markers. Immunoexpression ratios of these neuroendocrine markers were as follows: Follicular adenomas, neuron-specific enolase (NSE) 63.6%, synaptophysin (SynP) 45.5%, Leu7 27.3%, NCAM 45.5%, chromogranin A (CgA) 0%, SNAP25 0%; follicular carcinomas, NSE 90.0%, SynP 80.0%, Leu7 80.0%, NCAM 0%, CgA 0%, SNAP25 0%; papillary carcinomas, NSE 85.7%, SynP 78.6%, Leu7 100%, NCAM 7.0%, CgA 0%, SNAP25.0%; anaplastic carcinomas, NSE 10.0%, SynP 0%, Leu7 0%, NCAM 0%, CgA 0%, SNAP25 0%; medullary carcinomas, NSE 100%, SynP100%, Leu7 80.0%, NCAM 40.0%, CgA 100%, SNAP25 100%. The two follicular carcinomas, which were morphologically characterized by "insular" (or "alveolar") arrangements, showed distinct immunoexpression of NSE and SynP at the same time. By in situ hybridization (ISH), expression of mRNA for NSE was confirmed in cases with marked immunoexpression of NSE. Although no endocrine granules were found, our results suggested that a specific type of follicular carcinoma, i.e., insular variant, may be immaturely neuroendocrine-differentiated.

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References

  1. Chr, Hedinger. Definition and explanatory notes. In: Chr, Hedinger, ed. Histological typing of Thyroid tumours. 2nd ed. New York, Springer-Verlag, 5–14, 1993.

    Google Scholar 

  2. Ljungberg O, Boudeson L, Bondeson AG. Differentiated thyroid carcinoma, Intermediate type: A new tumor entity with feature of follicular and parafollicular cell carcinoma. 15:218–228, 1984.

  3. Beltrami CA, Barbatelli G, Criante P, et al. Immunohistochemical study in thyroid cancer. 5:229–245, 1987.

    Google Scholar 

  4. Kargi A, Yauraukoglu K, Aktas S, et al. Endocrine differentiation in non-neuroendocrine thyroid carcinoma. Thyroid 6:207–210, 1996.

    PubMed  CAS  Google Scholar 

  5. Ljungberg O, Ericsson UB, Bondeson L, et al. A compound follicular-parafollicular cell carcinoma of the thyroid: A new tumor entity? Cancer 52:1053–1061, 1983.

    Article  PubMed  CAS  Google Scholar 

  6. Kiji T, Branner RM. Localization of estrogen receptor messenger ribonucleic acid in rhesus monkey uterus by nonradioactive in situ hybridization with dioxigenin-labeled oligodeoxynucleotides. Endocrinology 132:382–392, 1993.

    Article  Google Scholar 

  7. Oliva D, Barba G, barbieri G, et al. Cloning expression and sequence homologies of cDNA for human gamma enolase. Gene 79:355–360, 1989.

    Article  PubMed  CAS  Google Scholar 

  8. Papotti M, Negro F, Carney JA, et al. Mixed medullary-follicular carcinoma of the thyroid. A morphological, immunohistochemical and in situ hybridization analysis of 11 cases. Virchow’s Arch 430:397–405, 1997.

    Article  CAS  Google Scholar 

  9. Wiedenmann B, Huttner WB. Synaptophysin and chromogranins/secregranins-widespread constituents of distinct types of neuroendocrine vesicles and new tools in tumor diagnosis. Virchows Arch [B] 58:95–121, 1989.

    Article  CAS  Google Scholar 

  10. Gould VE, Wiedenmann BG, Lee I, et al. Synaptophysin expression in neuroendocrine neoplasm as determined by immunocytochemistry. Am J Pathol 126:243–257, 1987.

    PubMed  CAS  Google Scholar 

  11. Khan A, Baker SP, Patwardhan NA, et al. CD57 (Leu 7) expression is helpful in diagnosis of the follicular variant of papillary thyroid carcinoma. Virchow Arch 432:427–432, 1998.

    Article  CAS  Google Scholar 

  12. Sasaki H, Yoshida K, Ikeda E, et al. Expression of the neural cell adhesion molecule in astrocytic tumors: an inverse correlation with malignancy. Cancer 82:1921–1931, 1998.

    Article  PubMed  CAS  Google Scholar 

  13. Kaufmann O, Georgi T, Dietel M. Utility of 123C3 monoclonal antibody against CD56 (NCAM) for the diagnosis of small cell carcinomas on paraffin sections. Hum Pathol 28:1373–1378, 1997.

    Article  PubMed  CAS  Google Scholar 

  14. Vargas F, Tolosa E, Sospedra M, et al. Characterization of neural cell adhesion molecule (NCAM) expression in thyroid follicular cells: induction by cytokines and over-expression in autoimmune glands. Clin Exp Immunol 98:478–488, 1994.

    Article  PubMed  CAS  Google Scholar 

  15. Majo G, Ferrer I, Marsal J, et al. Immunohistochemical analysis of the synaptic proteins SNAP-25 and Rab 3A in human pituitary adenomas. Overexpression of SNAP-25 in the mammosomatotroph lineages. J Pathol 183:440–446, 1997.

    Article  PubMed  CAS  Google Scholar 

  16. Risinger C, Bennett MK. Differential phosphorylation of syntaxin and synaptosome-associated protein of 25kDa (SNAP-25) isoforms. J Neurochem 72:614–624, 1999.

    Article  PubMed  CAS  Google Scholar 

  17. Fletcher L, Rider C, Taylor CB. Enolase isozymes III. Chromatographic and immunological characteristics of rat brain enolase. Biochim Biophys Acta 452:245–252, 1976.

    PubMed  CAS  Google Scholar 

  18. Verma M, Dutta SK. DNA sequences encoring enolase are remarkably conserved from yeast to mammals. Life Science 55:893–899, 1994.

    Article  CAS  Google Scholar 

  19. Rider CC and Taylor CB. Evidence for a new form of enolase in rat brain. Biochem Biophys Res Commun 66:814–187, 1975.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, School of Medicine, Tokai University, Bohseidai, Isehara, Kanagawa, Japan

    Fumiko Satoh MD, Shinobu Umemura MD, Masanori Yasuda MD & R. Yoshiyuki Osamura MD

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  1. Fumiko Satoh MD
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  2. Shinobu Umemura MD
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  3. Masanori Yasuda MD
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  4. R. Yoshiyuki Osamura MD
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Correspondence to Fumiko Satoh MD.

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Satoh, F., Umemura, S., Yasuda, M. et al. Neuroendocrine marker expression in thyroid epithelial tumors. Endocr Pathol 12, 291–299 (2001). https://doi.org/10.1385/EP:12:3:291

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