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Induction of RNA interference in dendritic cells

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Abstract

Dendritic cells (DC) reside at the center of the immunological universe, possessing the ability both to stimulate and inhibit various types of responses. Tolerogenic/regulatory DC with therapeutic properties can be generated through various means of manipulations in vitro and in vivo. Here we describe several attractive strategies for manipulation of DC using the novel technique of RNA interference (RNAi). Additionally, we overview some of our data regarding yet undescribed characteristics of RNAi in DC such as specific transfection strategies, persistence of gene silencing, and multi-gene silencing. The advantages of using RNAi for DC genetic manipulation gives rise to the promise of generating tailor-made DC that can be used effectively to treat a variety of immunologically mediated diseases.

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  1. Wei-Ping Min

    Present address: The JP Robarts Research Institute, 339 Windermere Road LHSC-UC 9L9, N6A 5A5, London, Ontario, Canada

Authors and Affiliations

  1. Department of Surgery, Microbiology and Immunology, The University of Western Ontario, London, Ontario, Canada

    Mu Li, Hua Qian, Thomas E. Ichim, Igor A. Popov, Katarzyna Rycerz, David White, Robert Zhong &  Wei-Ping Min

  2. Multi-Organ Transplant Program, London Health Science Centre, London, Ontario, Canada

    Mu Li, Hua Qian, Thomas E. Ichim, Igor A. Popov, David White, Robert Zhong &  Wei-Ping Min

  3. Immunology and Transplantation, Lawson Health Research Institute, London, Ontario, Canada

    John Neu, David White, Robert Zhong &  Wei-Ping Min

  4. The JP Robarts Research Institute, 339 Windermere Road LHSC-UC 9L9, N6A 5A5, London, Ontario, Canada

    Wei-Wen Ge, David White & Robert Zhong

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  1. Mu Li
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Li, M., Qian, H., Ichim, T.E. et al. Induction of RNA interference in dendritic cells. Immunol Res 30, 215–230 (2004). https://doi.org/10.1385/IR:30:2:215

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