Abstract
Organotypic slice cultures have been prepared from the brains of transgenic mice with Alzheimer’s disease-type pathology. Cell types within the slice undergo differentiation and slices can be maintained in culture for up to 6 mo when prepared from young neonates. Slices have been prepared from mice overexpressing genes of relevance to Alzheimer’s disease, including mutant or wild-type tau. Neurons in these slices develop neurons that are immunoreactive for a number of markers of abnormal tau. Organotypic slice models are currently being used to test the impact of tangle enhancers or inhibitors as a prescreen for efficacy before testing drugs in vivo.
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Duff, K., Noble, W., Gaynor, K. et al. Organotypic slice cultures from transgenic mice as disease model systems. J Mol Neurosci 19, 317–320 (2002). https://doi.org/10.1385/JMN:19:3:317
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DOI: https://doi.org/10.1385/JMN:19:3:317