Biophysical Journal
Volume 87, Issue 4, October 2004, Pages 2656-2662
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Protein Stiffening and Entropic Stabilization in the Subdenaturing Limit of Guanidine Hydrochloride

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Abstract

Subdenaturing concentrations of guanidine hydrochloride (GdnHCl) stabilize proteins. For ferrocytochrome c the stabilization is detected at subglobal level with no measured change in global stability. These deductions are made by comparing observed rates of thermally driven ferrocytochrome cHCO reactions with global unfolding rates of ferrocytochrome c measured by stopped flow and NMR hydrogen exchange in the presence of a wide range of GdnHCl concentrations at pH 7, 22 °C.

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This article is dedicated to the memory of Prof. Bhaskar G. Maiya.