Abstract
Objective: To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines. Methods: BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3–18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA; splenocytes were isolated for detecting specific CTL response and cytokine assayin vitro. Results: The anti-HBs antibody level of mice co-immunized with pcDNA3–18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone, but there was not significantly different (P>0.05). Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcDNA3–18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN-γ in supernatant of splenocytes cultured with HBsAgin vitro was significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05). Conclusion: The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant.
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Chen, Jz., Zhu, Hh., Liu, Kz. et al. Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant. J. Zheijang Univ.-Sci. 5, 467–471 (2004). https://doi.org/10.1631/jzus.2004.0467
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DOI: https://doi.org/10.1631/jzus.2004.0467