Abstract
Seashore landfill aquifers are environments of special physicochemical conditions (high organic load and high salinity), and microbes in leachate-polluted aquifers play a significant role for intrinsic bioremediation. In order to characterize microbial diversity and look for clues on the relationship between microbial community structure and hydrochemistry, a culture-independent examination of a typical groundwater sample obtained from a seashore landfill was conducted by sequence analysis of 16S rDNA clone library. Two sets of universal 16S rDNA primers were used to amplify DNA extracted from the groundwater so that problems arising from primer efficiency and specificity could be reduced. Of 74 clones randomly selected from the libraries, 30 contained unique sequences whose analysis showed that the majority of them belonged to bacteria (95.9%), with Proteobacteria (63.5%) being the dominant division. One archaeal sequence and one eukaryotic sequence were found as well. Bacterial sequences belonging to the following phylogenic groups were identified: Bacteroidetes (20.3%), β, γ, δ and ε-subdivisions of Proteobacteria (47.3%, 9.5%, 5.4% and 1.3%, respectively), Firmicutes (1.4%), Actinobacteria (2.7%), Cyanobacteria (2.7%). The percentages of Proteobacteria and Bacteroides in seawater were greater than those in the groundwater from a non-seashore landfill, indicating a possible influence of seawater. Quite a few sequences had close relatives in marine or hypersaline environments. Many sequences showed affiliations with microbes involved in anaerobic fermentation. The remarkable abundance of sequences related to (per)chlorate-reducing bacteria (CIRB) in the groundwater was significant and worthy of further study.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bond, P.L., Hugenholtz, P., Keller, J., Blackall, L., 1995. Bacterial community structures of phosphate-removing and non-phosphate-removing activated sludges from sequencing batch reactors.Applied and Environmental Microbiology,61:1910–1916.
Bowman, J.P., McCammon, S.A., Brown, M.V., Nichols, D.S., McMeekin, T.A., 1997. Diversity and association of psichrophilic bacteria in antarctic sea. ice.Applied and Environmental Microbiology,63:3068–3078.
Christensen, T.H., Kjeldsen, P., Albrechtsen, H.J., Heron, G., Nielsen, P.H., Bjerg, P.L., Holm, P.E., 1994. Attenuation of landfill leachate pollutants in aquifers.Critical Review on Environmental Science and Technology,24:119–202.
Coates, J.D., Michaelidou, U., Bruce, R.A., O–connor, S.M., Crespi, J.N., Achenbach, L.A., 1999. Ubiquity and diversity of dissimilatory (per)chlorate-reducing bacteria.Applied and Environmental Microbiology,65:5234–5241.
Li, L., Kato, C., Horikoshi, K., 1999. Bacterial diversity in the deep-sea sediments from different depths.Biodiversity Conservation,8:659–677.
Lovley, D.R., 2001. Anaerobes to the rescue.Science,293:1444–1446.
Martin-Laurent, F., Philippot, L., Hallet, S., Chaussod, R., Germon, J.C., Soulas, G., Catroux, G., 2001. DNA extraction from soils: old bias for new microbial diversity analysis methods.Applied and Environmental Microbiology,67:2354–2359.
Röling, W.F.M., Breukelen, B.M., Braster, M., Lin, B., Verseveld, H.W., 2001. Relationships between microbial community structure and hydrochemistry in a landfill leachate-polluted aquifer.Applied and Environmental Microbiology,67:4619–4629.
Rooney-Varga, J.N., Anderson, R.T., Fraga, J.L., Ringelberg, D., Lovley, D.R., 1999. Microbial communities associated with anaerobic benzene degradation in a petroleum-contaminated aquifer.Applied and Environmental Microbiology,65:3056–3063.
Song, B., Palleroni, N.J., Haggblom, M.M., 2000, Isolation and characterization of diverse halobenzoate-degrading denitrifying bacteria from soils and sediments.Applied and Environmental Microbiology,66:3446–3453.
Suzuki, M., Rappe, M.S., Giovannoni, S.J., 1998. Kinetic bias in estimates of coastal picoplankton community structure obtained by measurements of small-subunit rRNA gene PCR amplicon length heterogenity.Applied and Environmental Microbiology,64:4522–4529.
Todorov, J.R., Chistoserdov, A.Y., Aller, J.Y., 2000. Molecular analysis of microbial communities in mobile deltaic muds of Southeastern Papua New Guinea.FEMS Microbiology Ecology,33:147–155.
Wise, M.G., Mcarthur, J.V., Shimkets, L.J., 1997. Bacterial diversity of a carolina bay as determined by 16S rRNA gene analysis: confirmation of novel taxa.Applied and Environmental Microbiology,63:1505–1514.
Yanagibayashi, M., Nogi, Y., Li, L., Kato, C., 1999. Changes in the microbial community in Japan Trench sediment from a depth of 6292 m during cultivation without decompression.FEMS Microbiology Letter,170:271–279.
Yu, Z., Mohn, W.W., 2001. Bacterial diversity and community structure in an aerated lagoon revealed by ribosomal intergenic spacer analysis and 16S ribosomal DNA sequencing.Applied and Environmental Microbiology,67:1565–1574.
Author information
Authors and Affiliations
Corresponding author
Additional information
Project (No. 20377030) supported by the National Natural Science Foundation of China
Rights and permissions
About this article
Cite this article
Tian, Yj., Yang, H., Wu, XJ. et al. Molecular analysis of microbial community in a groundwater sample polluted by landfill leachate and seawater. J Zheijang Univ Sci B 6, 165–170 (2005). https://doi.org/10.1631/jzus.2005.B0165
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1631/jzus.2005.B0165