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Tissue Storage of 14C-Labelled Hydroxyethyl Starch (HES) 130/0.4 and HES 200/0.5 after Repeated Intravenous Administration to Rats

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Abstract

Objective: To investigate the effect of the molar substitution of hydroxyethyl starch (HES) solutions on tissue and organ storage in rats after repeated administration.

Study design: A controlled, multiple-dose study was performed in 48 rats. Daily bolus injections of 0.7 g/kg of 10% HES 130/0.4 or 10% HES 200/0.5 were administered on 18 consecutive days. In order to examine quantitatively the distribution and excretion of both preparations on HES tissue storage, radiolabelled HES was measured 3, 10, 24 and 52 days after the last administration. Tissue storage was expressed as percentage radioactivity of total administered HES dose in the total body, carcass, liver, kidney, spleen, lymph nodes, plasma and urine.

Results: 52 days after the last administration the remaining radioactivity of labelled HES 200/0.5 was nearly 4-fold higher compared with HES 130/0.4 in the total body (2.45% vs 0.65% of the total administered dose). This difference in tissue storage was statistically significant (p ≤ 0.01). A comparable difference was observed for the liver and carcass.

Conclusion: Tissue storage after repeated administration of an HES solution with a low molar substitution (0.4) was significantly lower in the total body as well as in the liver and carcass compared with an HES solution with a medium molar substitution (0.5). However, the potential clinical consequences of these present findings have not yet been determined.

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Acknowledgements

This study was sponsored by Fresenius Kabi Deutschland GmbH, Bad Homburg, Germany. The excellent technical assistance of K. Henning, R&D Fresenius Kabi Deutschland in preparing the 14C-labelled HES formulations is gratefully acknowledged.

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Authors and Affiliations

  1. LPT Laboratory of Pharmacology and Toxicology, Hamburg, Germany

    Jost Leuschner & Andreas Winkler

  2. Clinical Research, Fresenius Kabi Deutschland GmbH, 61348, Bad Homburg, Germany

    Jens Opitz, Roland Scharpf &  Frank Bepperling

Authors
  1. Jost Leuschner
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  2. Jens Opitz
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  3. Andreas Winkler
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  4. Roland Scharpf
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  5. Frank Bepperling
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Correspondence to Frank Bepperling.

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Leuschner, J., Opitz, J., Winkler, A. et al. Tissue Storage of 14C-Labelled Hydroxyethyl Starch (HES) 130/0.4 and HES 200/0.5 after Repeated Intravenous Administration to Rats. Drugs R&D 4, 331–338 (2003). https://doi.org/10.2165/00126839-200304060-00001

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