Evolution and virulence of Panton-Valentine leukocidin-positive ST30 methicillin-resistant Staphylococcus aureus in the past 30 years in Japan

Hirokazu Isobe; Tomomi Takano; Akihito Nishiyama; Wei-Chun Hung; Shuichi Kuniyuki; Yasuhiro Shibuya; Ivan Reva; Shizuka Yabe; Yasuhisa Iwao; Wataru Higuchi; Olga E Khokhlova; Takeshi Okubo; Tatsuo Yamamoto

doi:10.2220/biomedres.33.97

Full papers

Evolution and virulence of Panton-Valentine leukocidin-positive ST30 methicillin-resistant Staphylococcus aureus in the past 30 years in Japan

Hirokazu Isobe, Tomomi Takano, Akihito Nishiyama, Wei-Chun Hung, Shuichi Kuniyuki, Yasuhiro Shibuya, Ivan Reva, Shizuka Yabe, Yasuhisa Iwao, Wataru Higuchi, Olga E Khokhlova, Takeshi Okubo, Tatsuo Yamamoto

Author information

Hirokazu Isobe
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
Tomomi Takano
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
Akihito Nishiyama
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
Wei-Chun Hung
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
Shuichi Kuniyuki
Department of Dermatology, Osaka City General Hospital, Osaka, Japan
Yasuhiro Shibuya
Department of Respiratory Medicine, Tokyo Metropolitan Bokuto General Hospital, Tokyo, Japan
Ivan Reva
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
Shizuka Yabe
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
Yasuhisa Iwao
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
Wataru Higuchi
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
Olga E Khokhlova
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
Takeshi Okubo
Department of Respiratory Medicine, Niigata Prefectural Muikamachi Hospital, Niigata, Japan
Tatsuo Yamamoto
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan

JOURNAL FREE ACCESS

2012 Volume 33 Issue 2 Pages 97-109

DOI https://doi.org/10.2220/biomedres.33.97

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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) includes hospital-acquired MRSA (HAMRSA) and community-acquired MRSA (CA-MRSA). Panton-Valentine leukocidin (PVL)-positive multilocus sequence type 30 (ST30) MRSA is one of worldwide CA-MRSA, which has also persisted in Japan since the 1980s. However, unexpectedly, it was not the same ST30 clone throughout. Before 2000, it was HA-MRSA with spa43 and φSa3sea (phage Sa3 carrying the sea gene) and only one PVL-positive MRSA in Japan; in the 1980s, ST30 MRSA accounted for 23.5% of HA-MRSA, showed multidrug resistance, had high MICs for oxacillin and imipenem, and caused decubitus and pneumonia in hospitalized patients. A dynamic clonal change (spa43/φSa3sea→ spa19) occurred around 2000-2002. A rare spa43/φSa3sea/SCCmecI-IE25923 genotype also emerged. After 2002, the prevalent spa19 clone was CA-MRSA; it accounted for only 0.3% (or less) of MRSA in hospitals but 7.6% of CA-MRSA. Since 2007, PVL-positive CA-MRSA with other ST types (such as ST8, ST22, and ST59) also emerged in Japan, albeit at a low frequency. ST30/spa19 CA-MRSA occasionally caused severe invasive infections and a novel ST1335/spa19 genotype emerged. These ST30/spa19 CA-MRSA and variants were identified by pulsed field gel electrophoresis. Further analysis revealed that PVL-positive ST30/spa19 CA-MRSA is a highlyvirulent, successful clone, having a potential of clonal expansion.

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