IMR Press / FBL / Volume 12 / Issue 10 / DOI: 10.2741/2362

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Article
Surface chemistries for antibody microarrays
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1 Pacific Northwest National Laboratory, Biological Sciences Division, Richland, WA 99354, USA
Front. Biosci. (Landmark Ed) 2007, 12(10), 3956–3964; https://doi.org/10.2741/2362
Published: 1 May 2007
Abstract

Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection of disease biomarkers. The original technology for printing ELISA microarray chips and capturing antibodies on slides was derived from the DNA microarray field. However, due to the need to maintain antibody structure and function when immobilized, surface chemistries used for DNA microarrays are not always appropriate for ELISA microarrays. In order to identify better surface chemistries for antibody capture, a number of commercial companies and academic research groups have developed new slide types that could improve antibody function in microarray applications. In this review we compare and contrast the commercially available slide chemistries, as well as highlight some promising recent advances in the field.

Keywords
ELISA
Antibody
Microarray
Solid Support
Review
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