Journal of Nutritional Science and Vitaminology
Online ISSN : 1881-7742
Print ISSN : 0301-4800
ISSN-L : 0301-4800
Regular Paper
Transport of High Concentration of Thiamin, Riboflavin and Pyridoxine across Intestinal Epithelial Cells Caco-2
Magdalena ZIELINSKA-DAWIDZIAKKatarzyna GRAJEKAnna OLEJNIKKatarzyna CZACZYKWlodzimierz GRAJEK
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2008 Volume 54 Issue 6 Pages 423-429

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Abstract

In this study, we examined the intestinal uptake of thiamin (vitamin B1), riboflavin (vitamin B2) and pyridoxine (vitamin B6) administered at high concentration using intestinal epithelial Caco-2 cells as an in vitro model of drugs and food absorption. The effect of vitamin concentration, culture age, transport direction and incubation temperature on vitamin transport was determined. The vitamin transport was expressed as an apparent permeability coefficient and changes in cumulative fraction transported across epithelial membrane in time. It was found that transepithelial transport of these vitamins is dependent on the experimental factors. At low concentrations an active transport mechanism was observed, whereas at high vitamin concentration a passive transport dominated. At high vitamin concentration the transepithelial flux of vitamins in both directions was similar, which proves the mechanism of passive transport.

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© 2008 by the Center for Academic Publications Japan
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