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Review

Role of the Osmotic Stress Regulatory Pathway in Morphogenesis and Secondary Metabolism in Filamentous Fungi

1
Department of Biological Sciences, Lincoln Hwy. 1425W., Montgomery Hall, Northern Illinois University, DeKalb, IL 60115, USA
2
Food and Feed Safety Research Unit USDA, ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd, New Orleans, LA 70124, USA
*
Author to whom correspondence should be addressed.
Toxins 2010, 2(4), 367-381; https://doi.org/10.3390/toxins2040367
Submission received: 19 February 2010 / Revised: 16 March 2010 / Accepted: 17 March 2010 / Published: 24 March 2010
(This article belongs to the Special Issue Advances in Mycotoxin Research)

Abstract

:
Environmental stimuli trigger an adaptative cellular response to optimize the probability of survival and proliferation. In eukaryotic organisms from mammals to fungi osmotic stress, mainly through the action of the high osmolarity glycerol (HOG) pathway, leads to a response necessary for adapting and surviving hyperosmotic environments. In this review we show that the osmoadaptative response is conserved but not identical in different fungi. The osmoadaptative response system is also intimately linked to morphogenesis in filamentous fungi, including mycotoxin producers. Previous studies indicate that the response to osmotic stress is also coupled to the biosynthesis of natural products, including mycotoxins.

1. Introduction

Filamentous fungi are ubiquitous in nature, capable of inhabiting very diverse ecological niches. Many filamentous fungi propagate by means of dispersal of asexual spores termed conidia. Spores can traverse very long distances through the air which allows them to come to rest in environments that can be very different from that of their origin. In order for the fungus to proliferate and thrive in nature, it must be capable of adapting quickly to any particular environment it may find itself in. Even when acclimated to their particular niche, perturbations can occur from time to time in the surrounding environment either as a result of natural causes (i.e., drought) or man-made causes (i.e., modern agriculture). To adapt to such environmental changes fungi must continually adjust their physiology in order to enhance survival. At the molecular level, fungi possess complex signal transduction pathways that allow the fungus to respond appropriately to alterations in external stimuli. These stimuli can include biotic stress, and abiotic stresses such as changes in temperature, pH, nutrient availability, oxidative stress and osmotic stress. In this report we will be focusing on the role that osmotic stress plays in the growth and development of filamentous fungi and the molecular mechanisms that control the fungus’ response to alterations in osmotic pressure.
Many of the studies that first identified genes involved in the molecular regulation of cellular responses to osmotic pressure were performed in the yeast Saccharomyces cerevisiae (reviewed in [1]). These included the identification of MAP kinase signaling pathways and the high osmolarity glycerol (HOG) pathway involved in response to osmotic pressure [2]. In addition to providing information gleaned from studies using S. cerevisiae, we will also focus on research involving filamentous fungi, particularly of the genus Aspergillus. A. nidulans has been used extensively as a model fungus for the study of the molecular genetics of responses to osmotic stress due in large part to the availability of a vast number of genetic markers and a sexual stage that allows for genetic recombination studies [3,4]. A. nidulans also produces the mycotoxin sterigmatocystin, which is synthesized through the same conserved pathway that leads to production of aflatoxin in other Aspergilli. Both compounds are potent carcinogenic mycotoxins. In addition to A. nidulans, the genus Aspergillus includes a number of species that are of great importance both economically and medically. For example, the agriculturally important aflatoxin-producing species A. flavus and A. parasiticus [5]; A. oryzae and A. niger as industrially important sources of enzymes [6,7]; and medically important species such as the causal agent of human aspergillosis, the gliotoxin-producer A. fumigatus [8]. A number of studies have reported on the effects of osmotic stress on Aspergillus spp. as influenced by water content, water activity (Aw), and solute concentrations. Lillehoj et al. [9] reported on the effect of moisture (measured as percent moisture content or osmotic pressure) and substrate variation in developing cottonseed and corn. They found that osmotic pressure and other factors such as oil and starch content of seeds determine the physiological responses of A. parasiticus. Maximum accumulation of aflatoxin was observed in corn kernels and cottonseed that were inoculated at 52 and 70% moisture content, respectively. A water availability in the 600 kPa range of osmotic pressure provided optimum conditions for A. parasiticus development in corn kernels. Osmotic stress has also been shown to be a critical factor in enzyme production, relevant in industrial fermentations that utilize aspergilli. It was found that increased osmotic pressure as determined by NaCl concentration resulted in increased production and secretion of glucose oxidase during fermentation by A. niger [10]. Kobayashi et al. [11] found that glucoamylase activity by A. oryzae showed an increase of about 20-fold as water content of the wheat bran substrate was increased.
Scientists have made great strides in elucidating some of the basic molecular mechanisms that allow fungi to survive and proliferate under different environmental conditions. The fact that osmoadaptation is easy to manage and mimic in a lab setting has aided in studies of fungal responses to osmotic stress both at the cellular and molecular level. The advent of whole genome sequencing and functional genomics has also provided another tool to dissect the regulatory factors and signal transduction pathways that respond to osmotic stress. A recent review of annotated stress proteins by Miskei et al. [12] identified a number of genes in different Aspergillus species (A. nidulans, A. flavus, A fumigatus, A. niger, A. terreus, A. oryzae, A clavatus and N. fisheri), that are orthologous to those encoding components of the S. cerevisiae HOG pathway [2]. These studies have facilitated the generation of A. nidulans strains that harbor mutations in several members of the HOG pathway [13,14]. Miskei et al. [12] also proposed in this report that the response of the HOG pathway in filamentous fungi is not exclusively due to the fungus’ response to osmotic stress but also to oxidative stress. In this review we strive to present a compilation of information on the genes involved in the molecular regulation of the response to osmotic pressure in filamentous fungi mainly from studies with the model fungus A. nidulans and the model yeast S. cerevisiae. We also include an overview of the effect of osmotic stress on development and secondary metabolism in filamentous fungi, particularly those associated with mycotoxin production. In addition, we present information from our labs on the velvet gene (veA) and its role in filamentous fungi as a light-responsive factor that can integrate external stimuli, including osmotic stress to bring about physiological responses that are often manifested as alterations in secondary metabolism and/or morphogenesis.

2. Osmoadaptation Mechanisms in Yeast

Numerous studies conducted in the yeast S. cerevisiae have contributed to the understanding of the high osmolarity glycerol (HOG) pathway (reviewed in [1]). Activation of the HOG pathway depends on an increase in osmotic pressure resulting in a change in the expression of several genes. The molecular response to osmotic stress in S. cerevisiae is mediated by mitogen-activated protein kinase (MAPK) signaling pathways. Hog1p MAPK plays a central role in this signaling system, where Hog1p is activated by a two-component His-to-Asp phosphorelay system that includes Sln1p, a histidine kinase sensor; Ypd1p, a histidine-containing phosphotransfer protein; and Ssk1p and Skn7p response regulators [15,16] (Figure 1). Sln1p protein consists of an extracellular sensor, a kinase and a response regulator domain, giving a transmembrane hybrid-type histidine kinase. Under low-osmolarity, a specific histidine residue (His576) within the histidine kinase domain is autophosphorylated. Then, the phosphate group of the histidine kinase domain is transferred to an aspartate residue (Asp1144) within the receiver domain of Sln1p. Through a His-Asp phosphorelay, the phosphate group is transferred to the downstream Ypd1p phosphotransmitter, and then to the Ssk1p response regulator [12,15,17]. Phosphorylated Ssk1p fail to interact with the redundant pair of MAPKKK Ssk2p/Ssk22p, resulting in an inactive Ssk2p/Ssk22p-Pbs2p (MAPKK)-Hog1p form [18]. However, when yeast are exposed to osmotic stress, Sln1p is inhibited resulting in dephosphorylated forms of Ypd1p and Ssk1p, leading to Ssk1p-Ssk2p/Ssk22p interactions and finally the activation of Hog1p by phosphorylation [18]. In hyperosmotic environments, activated Hog1p migrates to the nucleus. This action is mediated by Gsp1p, a small GTP-binding protein, and the importin homologue Nmd5p; and it is independent of the NLS-binding importin/heterodimer [19]. Activation of the HOG pathway leads to the induction of genes required for osmotic stress response, for example glycerol biosynthesis genes such as the genes encoding a glycerol-3-phosphate dehydrogenase (GPD1) and glycerol-3-phosphatase (GPP2) [20,21].
A nuclear exchange sequence receptor protein called Crm1p exports dephosphorylated Hog1p back to the cytoplasm [19]. Negative regulation of Hog1p is exerted through phosphotyrosine phosphatases, Ptp2p and Ptp3p, in both the nucleus and the cytoplasm, respectively [22]; and the phosphatase Ptc1p, Ptc2p and Ptc3p [23,24]. Ptc1p dephosphorylates Hog1p via a docking interaction between Ptc1p, Hog1p, and Pbs2p joining a small adaptor named Nbp2p [25,26].
Figure 1. Comparison of S. cerevisiae (A) and A. nidulans (B) HOG regulatory pathways.
Figure 1. Comparison of S. cerevisiae (A) and A. nidulans (B) HOG regulatory pathways.
Toxins 02 00367 g001
In addition, in yeast Hog1p is regulated by a second mechanism, which includes a transmembrane sensor kinase called Sho1p (Figure 1). In the Sho1 branch, a multi-component signaling complex consisting of Sho1p, Cdc42p, Ste20p/Cla4p, Ste50p, Ste11p (MAPKKK), Pbs2p is formed in hyperosmotic environments. Pbs2p is localized to the membrane by its proline-based motif and an SH3 domain on Sho1. Pbs2p binds the MAPKKK Ste11p which is phosphorylated by the PAK-like kinase Ste20p, which is itself recruited to the membrane by activated Cdc42. Activated Ste11p then phosphorylates Pbs2p which in turn activates the downstream Hog pathway [26,27]. Hkr1p and Msb2p, mucin-like transmembrane proteins [27], are potential sensors for the Sho1p mechanism that activates Hog1p when the yeast is exposed to osmotic stress.
Activation of Hog1p can also lead to phosphorylation of the Sko1p bZip-type repressor, which results in the disassembly of Sko1p-Tup1p-Ssn6p repressor complexes [28]. Phosphorylated Hog1p interacts with the RCS chromatin-remodeling complex to mediate its recruitment to osmo-responsive genes [29]. Hog1p also recruits the Rpd3p histone deacetylase to osmoresponsive gene promoters inducing their expression during osmotic stress [30]. Hog1p is necessary for the activation and increase of the RNA polymerase II complex and mRNA, behaving as a transcriptional elongation factor specific for genes induced upon osmotic stress [29,31]. Phosphorylated Hog1p is also necessary for the activation of Msn2p/Msn4p C2H2 zinc finger transcription factors involved in general stress response [32,33,34,35].
Other Hog1p targets include Sgd1p, a novel essential nuclear protein. SGD1 was identified as a high-copy-number suppressor of the osmosensitive phenotype of pbs2D and hog1D deletion mutants [36,37]. In addition, Hog1p interacts and phosphorylates the transcription factor Smp1 upon osmotic stress controlling a subset of the responses induced by the MAPK [38]. Activated Hog1p can phosphorylate the Rck2p protein kinase, which participates in G2 checkpoint control and the osmotic stress triggered attenuation of protein synthesis [39,40].
In addition to the HOG signaling pathway, c-AMP dependent kinases have been shown to affect gene expression under osmotic stress [41]. However, this response is not exclusive to osmotic stress but a general stress response to a variety of additional stimuli such as nutrient starvation, heat shock, and oxidative stress among others [42,43,44]. Osmotic shock can also stimulate the production of compounds such as phosphatidylinositol-3,5-bisphosphate, which could participate as a second messenger in the activation of the osmotic signaling response [45].

3. Hog Pathway in Aspergillus nidulans

As in the case of S. cerevisiae, the HogA (SakA) pathway of A. nidulans (Figure 1) is activated in the osmotic [13,14,46,47,48] and oxidative [46,47] response by the A. nidulans Ssk1p ortholog, SskA. Interestingly, a sakA null mutant presented only slight sensitivity to high osmolarity stress, indicating that osmoregulation in A. nidulans differs from that described in yeast [14,46]. Other upstream components of this pathway are the Sln1p homolog, TcsB [49]; the Ypd1p ortholog, YpdA [14,48]; and also NikA, a dispensible Mak2-type histidine kinase in the osmotic stress response that has been demonstrated to transmit fungicide-induced stress signals such as those generated in the presence of fludioxonil and iprodione [48,50] (Figure 1). A tcsB deletion (tcsB) mutant did not present an osmosensitive phenotype [51] most likely due to redundancy of function with other histidine kinase genes present in A. nidulans genome [48,52]. However, the A. nidulans TcsB–YpdA–SskA system might have a role similar to that of Sln1p–Ypd1p–Ssk1p proteins in yeast [14]. A non-essential sensor kinase, TcsA, may be involved in conidiation [53] however, a TcsA–YpdA–response regulator signalling pathway has not been demonstrated.
Interestingly, it is likely that the Sho1p signaling pathway in A. nidulans might not be involved in osmoregulation but instead may be carrying out other signaling functions [54]. A. nidulans PbsB (homolog to S. cerevisiae Pbs1p) lacks a typical Pro-rich motif necessary for the binding with Sho1p [14,55]. Furthermore, proteins such as ModA, a Cdc42p-like protein [56], similar to Sho1p, have a role in morphogenesis. SteC, similar to Ste11p, regulates sexual development [57]. Differently from yeast, A. nidulans PbsB MAPKK activates another Hog1p ortholog, MpkC, not present in yeast. Although MpkC is not necessary for osmoregulation, overexpression of mpkC suppresses the slight high-osmolarity sensitivity of hogA strains [14]. An A. nidulans orthologue of S. cerevisiae Skn7p, SrrA, plays a role in both oxidative stress and osmotic stress resistance [47,48].
Little is known about SakA and YpdA import/export to the nucleus, although putative orthologues of Gsp1p (RanA), Nmd5p importin and Crm1p (KapK) export factor have be identified [12]. Dephosphorylation of HogA/SakA in A. nidulans is mainly unknown, except for the up-regulation of ptpA (encoding a putative protein phosphatase) when the fungus is exposed to osmotic stress [13]. Several SakA-dependent regulators have been identified, such as RpdA (Rpd3p ortholog), AtfA (putative ortholog of S. pombe Atf1) [58,59], and RcoA (S. cerevisiae Tup1 ortholog). Msn2p/Msn4p-type protein, MsnA, has been found in A. nidulans, and was shown to be induced by different types of stress [13]. In A. nidulans, potential target genes of SakA and SrrA (S. cerevisiae Skn7p homolog) includes gfdB (S. cerevisiae GPD2 ortholog) [13,60] and enaA [13].
The process of osmotic adaptation by activation of the HOG pathway, in both yeast and filamentous fungi, results in the biosynthesis and accumulation of compatible molecules such as proline, trehalose, polyols and glycerol to counterbalance the osmotic pressure and prevent loss of water. In A. nidulans, SskA (S. cerevisiae Ssk1p homolog) regulates the expression of genes involved in conidial tolerance to stress, including genes involved in glycerol and trehalose metabolism (gfdA and gfdB, glycerol-3-phosphate dehydrogenases; gldB, glycerol dehydrogenase; tpsA, trehalose-6-phosphate synthase; orlA, trehalose-6-phosphate phosphatase; treB, neutral trehalase) [13,61,62,63,64,65].

4. Examples of the Osmoadaptation Signaling Pathway in Other Aspergilli

Regulatory pathways including those signaling pathways responsive to stress are relatively conserved, but not identical, in the genus Aspergillus [12]. For example, conserved SakA plays an important role in the oxidative stress response in the opportunistic human pathogen and gliotoxin-producer Aspergillus fumigatus, while the TcsB histidine kinase was not crucial [66]. In A. fumigatus, MpkC plays other roles such as in carbon source utilization, however it was not determined to be involved in osmoadaptation [67]. Aspergillus niger, an important citric acid, gluconic acid and hydrolytic enzyme producing fungus, does not have TcsB-type histidine kinase homologs. Other aspergilli, such as A. nidulans mentioned above, present TscB but it is dispensable in the osmoadaptation response. It is likely that TcsB is dispensable or not present in other aspergilli [51,66].
Interestingly, the important aflatoxin-producer Aspergillus flavus harbors two orthologs of the A. nidulans SskA response regulator in its genome (ORFs AFL2G_06337 and AFL2G_12585), and two orthologs of the A. nidulans ortholog RpdA histone deacetylase (ORFs AFL2G_08263 and AFL2G_03062). This suggests that A. flavus has a more sophisticated osmotadaptation system compared to other aspergilli that may include genes with redundant functions.

5. Role of the Osmotic Stress-response Pathway on Fungal Development and Secondary Metabolism

The study of the HOG pathway and its implications in morphological development and secondary metabolism in filamentous fungi are still at the earliest stages. In A. nidulans, a sakA deletion mutant shows development and cell-specific phenotypes [46]. This mutant is characterized by premature sexual development. In addition, deletion of sakA results in conidia that are highly sensitive to oxidative and heat shock stress and lose viability overtime. This evidence supports the role of SakA as a repressor of sexual development and asexual spore stress resistance and survival. As mentioned in section 3, another member of the A. nidulans HOG pathway, SskA is important in regulating the tolerance of conidia to osmotic stress. SakA was also shown to be involved in conidia viability in A. fumigatus, where the sakA mutant presented alterations in conidia germination in a nitrogen source-dependent manner [68]. The bZip-type transcription factor AtfA (putative ortholog of S. pombe Atf1), that functions downstream of the HogA MAPK in the course of fludioxonil and osmotic stress response, is also involved in conidia stress tolerance [58,59,69,70]. Also in A. fumigatus, mutations in MA21, a homolog of the S. cerevisiae transmembrane sensor kinase SHO gene, showed a reduction in growth and germination rates. It demonstrated irregular hyphal morphology with a decrease in phialides and conidia [71]. Development is also affected by osmoregulation in dimorphic fungi such as Candida albicans, where the HOG homolog is necessary for chlamydospore formation [72].
Additionally, studies on A. nidulans SteC revealed its role in morphogenesis. Deletion of the steC gene results in a slower growth rate, increase of branched hyphae, alteration of conidiophore morphology, inhibition of heterokaryon formation and a blockage of cleistothecial development. The gene is transcriptionally activated during asexual development and controls the phosphorylation of two putative MAP kinases [57]. Also, RcoA (homolog of S. cerevisiae Tup1p) affects growth and sexual/asexual development in A. nidulans [73,74]. The response regulators SrrA and SskA are not only involved in osmotic and oxidative stress signal transduction but also in regulation of asexual development in A. nidulans. Deletion of any one of these genes results in a reduction in conidiation and spore viability [48].
The evidence provided by A. nidulans studies as well as studies in other fungi clearly indicates a connection between the osmotic stress regulatory system and fungal development. Recently in our laboratories we found that osmotic stress caused by high concentrations of sodium chloride, sorbitol or potassium chloride positively affects vegetative growth leads to an increase in conidiation in A. flavus (Duran et al., manuscript in preparation). Han et al. [75] also reported an increase of conidiation in the presence of high amount of sorbitol in A. nidulans. In contrast, in a study by Mert and Ekmekci [76] where A. flavus was inoculated on NaCl medium, a reduction of conidia was observed. The A. flavus strains are genetically diverse and they have been sub-divided into two strain types, S and L, based differences in sclerotial size [77]. It is possible that this variability in conidiation could be the result of different A. flavus strains used in these studies leading to different responses to osmotic stress and salinity. It is also possible that other culture conditions could have caused the observed differences in conidiation under induced osmotic stress. In addition, our recent study showed the light-dependent global regulator VeA, known to control sexual/asexual morphogenesis and secondary metabolism in filamentous fungi [78], to be associated with the osmotic-stress response in A. flavus (Duran et al., manuscript in preparation and Figure 1). Specifically, osmostress enhances hyperconidiation in the A. flavus veA deletion strain, suggesting that veA is involved in modulating osmotic stress-induced conidiation. On the other hand, salinity stress has been shown to have an inhibitory effect on the formation of sclerotia in fungal species, including Sclerotinia sclerotiorum, Rhizoctonia solani and Sclerotium rolfsii [79]. In the mycotoxin producer Aspergillus ochraceus osmotic stress had little effect on sclerotia production, [80]. In Botrytis cinerea sclerotia production was also reduced as osmotic stress increased [81]. In A. flavus, hyperosmotic media caused a delay in sclerotial maturation (Duran et al., manuscript in preparation). These studies suggest that under stress caused by hyperosmolarity the fungus favors investment of material and energy towards developmental programs (conidiation) that are conducive to survival via dissemination to a more favorable environment versus survival in the unfavorable hyperosmotic environment.
Previous studies support an association of fungal morphogenesis with secondary metabolism, including mycotoxin production [82]. Aflatoxin is one of the most potent natural carcinogenic compounds described. Surprisingly, hyperosmotic levels of NaCl, KCl or sorbitol did not affect the biosynthesis of this mycotoxin (Duran et al., manuscript in preparation). Some osmotic stress genetic response elements have been demonstrated to be linked to toxin production in A. nidulans, specifically, RcoA (Tup1p homolog) affects not only fungal growth and development, but also is necessary for the production of sterigmatocystin in A. nidulans [83]. Additionally, osmotic stress does affect the production of other secondary metabolites in aspergilli, for example pigments. The A. fumigatus sakA deletion mutant is unable to produce pigmentation as observed in the wild-type strain, indicating that secondary metabolism is affected by the HOG signaling pathway in this fungus [68].
Across fungal genera, the role of MAPK cascades in the osmotic stress response is quite diverse. In the filamentous fungus Fusarium graminearum, a significant relationship between osmotic stress and secondary metabolism has been observed. F. graminearum is a common pathogen of grain producing crops. Among the several secondary metabolites generated by this fungus, the synthesis of trichothecene toxins and a reddish colored pigment called aurofusarin are affected by osmotic stress. Ochiai et al. [84] described the participation of several histidine kinases, components of the osmotic response signalling pathway, in the regulation of secondary metabolism of F. graminearum. These authors showed that production of trichothecenes is markedly suppressed by NaCl, without a significant effect on fungal growth. A null mutant of FgOs1 (encoding the osmosensor histidine kinase) produced a reduced amount of the red pigment aurofusarin however it was unaltered in its ability to produce trichothecenes. Deletion null mutants of FgOs4 (encoding MAPKKK), FgOs5 (MAPKK), and FgOs2 (MAPK) all showed markedly enhanced pigmentation and failed to produce trichothecenes, coinciding with a marked reduction of expression of Tri4 and Tri6 (trichothecene biosynthetic pathway and regulatory genes). In the maize pathogen, Cochliobolus heterostrophus, hog1 mutants are more pigmented than the wild-type and demonstrate smaller appressoria and reduced virulence [85]. In cpmk1 (hog1 homolog) mutants of Cryphonectria parasitica, there was an increase in osmosensitivity along with reduced pigmentation, conidiation, and virulence on chestnut trees compared to a wild-type strain [86] In the rice pathogen, Magnaporthe grisea, osm1 (hog1 homolog) mutants were more sensitive to osmotic stress and showed some morphological defects. However, glycerol accumulation and appressorial turgor generation was unaltered compared to wild-type and virulence was not affected [87].

6. Conclusions

The ability to adapt is essential for filamentous fungi and yeast to survive and proliferate under non-optimal osmotic environments. Fungi sense and then transduce external changes in osmotic pressure mainly through cellular signaling pathways such as the HOG pathway that is mediated by MAPK cascades. Activated MAPKs phosphorylate a number of substrates including transcriptional activators that in turn modulate patterns of gene expression and subsequent protein synthesis. In fungi, responses to osmotic stress include the production of osmoprotectant compounds such as glycerol, reorganization of the cytoskeleton, and cell wall biogenesis. A fungus’ response to high osmotic pressures in an artificial environment such as during an industrial fermentation that utilizes controlled environmental and nutritional parameters is fairly straightforward. However, during a host-fungal pathogen interaction, the degree to which a fungus’ response to changes in osmotic pressure impacts its ability to successfully invade and survive in the host is not so clear. During the infection process, a pathogenic fungus would be expected to encounter a complex nutritional milieu upon lysis of plant cells that may include increased osmotic pressure. The entomopathogenic fungus, Metarhizium anisopliae, kills insects by direct penetration of the cuticle followed by multiplication in the hemolymph. The solute-rich hemolymph is characterized by high osmotic pressure [88] that could initiate a stress response in the fungus. M. anisopliae strains that were defective in the MOS1 osmosensor (SHO1 in yeast) displayed increased sensitivity to osmotic pressure as well as reduced virulence against larvae of Manduca sexta [89]. In the case of a plant necrotophic fungus, the breakdown of cell walls by fungal hydrolases and the generalized hypersensitive response by the host plant may result in the invading fungus being isolated in a region of high osmotic pressure. In addition, activation of the Hog1 MAPK pathway has been observed in Candida albicans cells exposed to the human antimicrobial peptide, histantin 5 [90]. It is possible that antimicrobial compounds elicited by the host may also trigger activation of the osmotic stress response in an invading plant or human pathogenic fungus leading to accumulation of osmolytes such as glycerol and reorganization of cell wall structure in an attempt to stave off death. With the ever increasing number of organisms whose genomes and proteomes have now been sequenced and annotated, it should be possible to better elucidate the impact that genes involved in the osmotic stress response have on host-fungal pathogen interactions.
The literature cited in this review indicates that the osmoadaptative response is conserved but not identical in different fungi and that it is intimately linked to morphogenesis. The ability to alter morphogenic programs in response to changes in osmotic pressure can play a significant role in the fungus’ capacity to survive and flourish in nature. One developmental response to an increase in osmotic stress often observed in fungi is an increase in conidiation. This increases the fungus’ chances of survival as conidia can be dispersed by wind over wide areas thus removing the fungus from an inhospitable environment and potentially allowing it to take up residence in a more favorable environment. Furthermore, some of the studies mentioned in this review have also shown that in some cases the response to osmotic stress by fungi is linked to the biosynthesis of natural products, including mycotoxins. However, examples of this are few and more studies will be necessary to gain further insight into the interaction between osmotic stress regulation and secondary metabolism.

Acknowledgements

This work was supported by Northern Illinois University.

References

  1. Hohmann, S.; Krantz, M.; Nordlander, B. Yeast osmoregulation. Methods Enzymol. 2007, 428, 29–45. [Google Scholar] [PubMed]
  2. Hohmann, S. Osmotic stress signaling and osmoadaptation in yeasts. Microbiol. Mol. Biol. Rev. 2002, 66, 300–372. [Google Scholar] [PubMed]
  3. Todd, R.B.; Davis, M.A.; Hynes, M.J. Genetic manipulation of Aspergillus nidulans: Meiotic progeny for genetic analysis and strain construction. Nat. Protoc. 2007, 2, 811–821. [Google Scholar] [PubMed]
  4. Bennett, J.W. Aspergillus: A primer for the novice. Med. Mycol. 2009, 47, S5–S12. [Google Scholar] [CrossRef] [PubMed]
  5. Cary, J.W.; Linz, J.E.; Bhatnagar, D. Aflatoxins: Biological significance and regulation of biosynthesis. In Microbial Foodborne Diseases: Mechanisms of Pathogenesis and Toxin Synthesis; Cary, J.W., Linz, J.E., Bhatnagar, D., Eds.; Technomic Publishing Co.: Lancaster, PA, USA, 2000; pp. 317–361. [Google Scholar]
  6. Roukas, T. Citric and gluconic acid production from fig by Aspergillus niger using solid-state fermentation. J. Ind. Microbiol. Biotechnol. 2000, 25, 298–304. [Google Scholar] [PubMed]
  7. Machida, M.; Yamada, O.; Gomi, K. Genomics of Aspergillus oryzae: Learning from the history of Koji mold and exploration of its future. DNA Res. 2008, 15, 173–183. [Google Scholar] [PubMed]
  8. Latge, J.P. Aspergillus fumigatus and aspergillosis. Clin. Microbiol. Rev. 1999, 12, 310–350. [Google Scholar] [PubMed]
  9. Lillehoj, E.B.; Wall, J.H.; Bowers, E.J. Preharvest aflatoxin contamination: Effect of moisture and substrate variation in developing cottonseed and corn kernels. Appl. Environ. Microbiol. 1987, 53, 584–586. [Google Scholar] [PubMed]
  10. Fiedurek, J. Effect of osmotic stress on glucose oxidase production and secretion by Aspergillus niger. J. Basic Microbiol. 1998, 38, 107–112. [Google Scholar] [PubMed]
  11. Kobayashi, A.; Sano, M.; Oda, K.; Hisada, H.; Hata, Y.; Ohashi, S. The glucoamylase-encoding gene (glaB) is expressed in solid-state culture with a low water content. Biosci. Biotechnol. Biochem. 2007, 71, 1797–1799. [Google Scholar] [PubMed]
  12. Miskei, M.; Karanyi, Z.; Pocsi, I. Annotation of stress-response proteins in the aspergilli. Fungal Genet. Biol. 2009, 46, S105–S120. [Google Scholar] [PubMed]
  13. Han, K.H.; Prade, R.A. Osmotic stress-coupled maintenance of polar growth in Aspergillus nidulans. Mol. Microbiol. 2002, 43, 1065–1078. [Google Scholar] [PubMed]
  14. Furukawa, K.; Hoshi, Y.; Maeda, T.; Nakajima, T.; Abe, K. Aspergillus nidulans HOG pathway is activated only by two-component signalling pathway in response to osmotic stress. Mol. Microbiol. 2005, 56, 1246–1261. [Google Scholar] [PubMed]
  15. Posas, F.; Wurgler-Murphy, S.M.; Maeda, T.; Witten, E.A.; Thai, T.C.; Saito, H. Yeast HOG1 MAP kinase cascade is regulated by a multistep phosphorelay mechanism in the SLN1-YPD1-SSK1 "two-component" osmosensor. Cell 1996, 86, 865–875. [Google Scholar] [PubMed]
  16. Li, S.; Ault, A.; Malone, C.L.; Raitt, D.; Dean, S.; Johnston, L.H.; Deschenes, R.J.; Fassler, J.S. The yeast histidine protein kinase, Sln1p, mediates phosphotransfer to two response regulators, Ssk1p and Skn7p. EMBO J. 1998, 17, 6952–6962. [Google Scholar] [PubMed]
  17. Maeda, T.; Wurgler-Murphy, S.M.; Saito, H. A two-component system that regulates an osmosensing MAP kinase cascade in yeast. Nature 1994, 369, 242–245. [Google Scholar] [PubMed]
  18. Posas, F.; Saito, H. Activation of the yeast SSK2 MAP kinase kinase kinase by the SSK1 two-component response regulator. EMBO J. 1998, 17, 1385–1394. [Google Scholar] [PubMed]
  19. Ferrigno, P.; Posas, F.; Koepp, D.; Saito, H.; Silver, P.A. Regulated nucleo/cytoplasmic exchange of HOG1 MAPK requires the importin beta homologs NMD5 and XPO1. EMBO J. 1998, 17, 5606–5614. [Google Scholar] [PubMed]
  20. Albertyn, J.; Hohmann, S.; Thevelein, J.M.; Prior, B.A. GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway. Mol. Cell Biol. 1994, 14, 4135–4144. [Google Scholar] [PubMed]
  21. Norbeck, J.; Pahlman, A.K.; Akhtar, N.; Blomberg, A.; Adler, L. Purification and characterization of two isoenzymes of DL-glycerol-3-phosphatase from Saccharomyces cerevisiae. Identification of the corresponding GPP1 and GPP2 genes and evidence for osmotic regulation of Gpp2p expression by the osmosensing mitogen-activated protein kinase signal transduction pathway. J. Biol. Chem. 1996, 271, 13875–13881. [Google Scholar] [PubMed]
  22. Mattison, C.P.; Ota, I.M. Two protein tyrosine phosphatases, Ptp2 and Ptp3, modulate the subcellular localization of the Hog1 MAP kinase in yeast. Gene. Develop. 2000, 14, 1229–1235. [Google Scholar]
  23. Warmka, J.; Hanneman, J.; Lee, J.; Amin, D.; Ota, I. Ptc1, a type 2C Ser/Thr phosphatase, inactivates the HOG pathway by dephosphorylating the mitogen-activated protein kinase Hog1. Mol. Cell Biol. 2001, 21, 51–60. [Google Scholar] [CrossRef] [PubMed]
  24. Young, C.; Mapes, J.; Hanneman, J.; Al-Zarban, S.; Ota, I. Role of Ptc2 type 2C Ser/Thr phosphatase in yeast high-osmolarity glycerol pathway inactivation. Eukaryot Cell 2002, 1, 1032–1040. [Google Scholar] [PubMed]
  25. Mapes, J.; Ota, I.M. Nbp2 targets the Ptc1-type 2C Ser/Thr phosphatase to the HOG MAPK pathway. EMBO J. 2004, 23, 302–311. [Google Scholar] [PubMed]
  26. Saito, H.; Tatebayashi, K. Regulation of the osmoregulatory HOG MAPK cascade in yeast. J. Biochem. 2004, 136, 267–272. [Google Scholar] [PubMed]
  27. Tatebayashi, K.; Yamamoto, K.; Tanaka, K.; Tomida, T.; Maruoka, T.; Kasukawa, E.; Saito, H. Adaptor functions of Cdc42, Ste50, and Sho1 in the yeast osmoregulatory HOG MAPK pathway. EMBO J. 2006, 25, 3033–3044. [Google Scholar] [CrossRef] [PubMed]
  28. Proft, M.; Pascual-Ahuir, A.; de Nadal, E.; Arino, J.; Serrano, R.; Posas, F. Regulation of the Sko1 transcriptional repressor by the Hog1 MAP kinase in response to osmotic stress. EMBO J. 2001, 20, 1123–1133. [Google Scholar] [PubMed]
  29. Mas, G.; de Nadal, E.; Dechant, R.; Rodriguez de la Concepcion, M.L.; Logie, C.; Jimeno-Gonzalez, S.; Chavez, S.; Ammerer, G.; Posas, F. Recruitment of a chromatin remodelling complex by the Hog1 MAP kinase to stress genes. EMBO J. 2009, 28, 326–336. [Google Scholar] [PubMed]
  30. De Nadal, E.; Zapater, M.; Alepuz, P.M.; Sumoy, L.; Mas, G.; Posas, F. The MAPK Hog1 recruits Rpd3 histone deacetylase to activate osmoresponsive genes. Nature 2004, 427, 370–374. [Google Scholar] [PubMed]
  31. Alepuz, P.M.; de Nadal, E.; Zapater, M.; Ammerer, G.; Posas, F. Osmostress-induced transcription by Hot1 depends on a Hog1-mediated recruitment of the RNA Pol II. EMBO J. 2003, 22, 2433–2442. [Google Scholar] [PubMed]
  32. Moskvina, E.; Schuller, C.; Maurer, C.T.; Mager, W.H.; Ruis, H. A search in the genome of Saccharomyces cerevisiae for genes regulated via stress response elements. Yeast 1998, 14, 1041–1050. [Google Scholar] [PubMed]
  33. Causton, H.C.; Ren, B.; Koh, S.S.; Harbison, C.T.; Kanin, E.; Jennings, E.G.; Lee, T.I.; True, H.L.; Lander, E.S.; Young, R.A. Remodeling of yeast genome expression in response to environmental changes. Mol. Biol. Cell 2001, 12, 323–337. [Google Scholar] [PubMed]
  34. Gasch, A.P. Comparative genomics of the environmental stress response in ascomycete fungi. Yeast 2007, 24, 961–976. [Google Scholar] [PubMed]
  35. O'Rourke, S.M.; Herskowitz, I.; O'Shea, E.K. Yeast go the whole HOG for the hyperosmotic response. Trends Genet. 2002, 18, 405–412. [Google Scholar] [PubMed]
  36. Akhtar, N.; Pahlman, A.K.; Larsson, K.; Corbett, A.H.; Adler, L. SGD1 encodes an essential nuclear protein of Saccharomyces cerevisiae that affects expression of the GPD1 gene for glycerol 3-phosphate dehydrogenase. FEBS Lett. 2000, 483, 87–92. [Google Scholar] [PubMed]
  37. Lin, H.; Nguyen, P.; Vancura, A. Phospholipase C interacts with Sgd1p and is required for expression of GPD1 and osmoresistance in Saccharomyces cerevisiae. Mol. Genet. Genomics. 2002, 267, 313–320. [Google Scholar] [PubMed]
  38. de Nadal, E.; Casadome, L.; Posas, F. Targeting the MEF2-like transcription factor Smp1 by the stress-activated Hog1 mitogen-activated protein kinase. Mol. Cell. Biol. 2003, 23, 229–237. [Google Scholar] [PubMed]
  39. Bilsland-Marchesan, E.; Arino, J.; Saito, H.; Sunnerhagen, P.; Posas, F. Rck2 kinase is a substrate for the osmotic stress-activated mitogen-activated protein kinase Hog1. Mol. Cell. Biol. 2000, 20, 3887–3895. [Google Scholar] [PubMed]
  40. Teige, M.; Scheikl, E.; Reiser, V.; Ruis, H.; Ammerer, G. Rck2, a member of the calmodulin-protein kinase family, links protein synthesis to high osmolarity MAP kinase signaling in budding yeast. Proc. Natl. Acad. Sci. USA 2001, 98, 5625–5630. [Google Scholar] [CrossRef]
  41. Norbeck, J.; Blomberg, A. The level of cAMP-dependent protein kinase A activity strongly affects osmotolerance and osmo-instigated gene expression changes in Saccharomyces cerevisiae. Yeast 2000, 16, 121–137. [Google Scholar] [PubMed]
  42. Marchler, G.; Schuller, C.; Adam, G.; Ruis, H. A Saccharomyces cerevisiae UAS element controlled by protein kinase A activates transcription in response to a variety of stress conditions. EMBO J. 1993, 12, 1997–2003. [Google Scholar] [PubMed]
  43. Ruis, H.; Schuller, C. Stress signaling in yeast. Bioessays 1995, 17, 959–965. [Google Scholar] [PubMed]
  44. Siderius, M.M.; Mager, W.H. The general stress response in search for a common denominator. In Yeast Stress Responses, 1st; Hohmann, S.M., Mager, W.H., Eds.; R.G. Landes Company: Austin, TX, USA, 1997; pp. 213–230. [Google Scholar]
  45. Dove, S.K.; Cooke, F.T.; Douglas, M.R.; Sayers, L.G.; Parker, P.J.; Michell, R.H. Osmotic stress activates phosphatidylinositol-3,5-bisphosphate synthesis. Nature 1997, 390, 187–192. [Google Scholar] [PubMed]
  46. Kawasaki, L.; Sanchez, O.; Shiozaki, K.; Aguirre, J. SakA MAP kinase is involved in stress signal transduction, sexual development and spore viability in Aspergillus nidulans. Mol. Microbiol. 2002, 45, 1153–1163. [Google Scholar] [CrossRef] [PubMed]
  47. Hagiwara, D.; Asano, Y.; Marui, J.; Furukawa, K.; Kanamaru, K.; Kato, M.; Abe, K.; Kobayashi, T.; Yamashino, T.; Mizuno, T. The SskA and SrrA response regulators are implicated in oxidative stress responses of hyphae and asexual spores in the phosphorelay signaling network of Aspergillus nidulans. Biosci. Biotechnol. Biochem. 2007, 71, 1003–1014. [Google Scholar] [PubMed]
  48. Vargas-Perez, I.; Sanchez, O.; Kawasaki, L.; Georgellis, D.; Aguirre, J. Response regulators SrrA and SskA are central components of a phosphorelay system involved in stress signal transduction and asexual sporulation in Aspergillus nidulans. Eukaryotic Cell 2007, 6, 1570–1583. [Google Scholar] [PubMed]
  49. Catlett, N.L.; Yoder, O.C.; Turgeon, B.G. Whole-genome analysis of two-component signal transduction genes in fungal pathogens. Eukaryotic Cell 2003, 2, 1151–1161. [Google Scholar] [PubMed]
  50. Hagiwara, D.; Matsubayashi, Y.; Marui, J.; Furukawa, K.; Yamashino, T.; Kanamaru, K.; Kato, M.; Abe, K.; Kobayashi, T.; Mizuno, T. Characterization of the NikA histidine kinase implicated in the phosphorelay signal transduction of Aspergillus nidulans, with special reference to fungicide responses. Biosci. Biotechnol. Biochem. 2007, 71, 844–847. [Google Scholar] [PubMed]
  51. Furukawa, K.; Katsuno, Y.; Urao, T.; Yabe, T.; Yamada-Okabe, T.; Yamada-Okabe, H.; Yamagata, Y.; Abe, K.; Nakajima, T. Isolation and functional analysis of a gene, tcsB, encoding a transmembrane hybrid-type histidine kinase from Aspergillus nidulans. Appl. Environ. Microbiol. 2002, 68, 5304–5310. [Google Scholar] [CrossRef] [PubMed]
  52. Suzuki, A.; Kanamaru, K.; Azuma, N.; Kato, M.; Kobayashi, T. GFP-tagged expression analysis revealed that some histidine kinases of Aspergillus nidulans show temporally and spatially different expression during the life cycle. Biosci. Biotechnol. Biochem. 2008, 72, 428–434. [Google Scholar] [CrossRef] [PubMed]
  53. Virginia, M.; Appleyard, C.L.; McPheat, W.L.; Stark, M.J. A novel 'two-component' protein containing histidine kinase and response regulator domains required for sporulation in Aspergillus nidulans. Curr. Genet. 2000, 37, 364–372. [Google Scholar] [PubMed]
  54. Krantz, M.; Becit, E.; Hohmann, S. Comparative genomics of the HOG-signalling system in fungi. Curr. Genet. 2006, 49, 137–151. [Google Scholar] [PubMed]
  55. Zarrinpar, A.; Park, S.H.; Lim, W.A. Optimization of specificity in a cellular protein interaction network by negative selection. Nature 2003, 426, 676–680. [Google Scholar] [PubMed]
  56. Virag, A.; Lee, M.P.; Si, H.; Harris, S.D. Regulation of hyphal morphogenesis by cdc42 and rac1 homologues in Aspergillus nidulans. Mol. Microbiol. 2007, 66, 1579–1596. [Google Scholar] [PubMed]
  57. Wei, H.; Requena, N.; Fischer, R. The MAPKK kinase SteC regulates conidiophore morphology and is essential for heterokaryon formation and sexual development in the homothallic fungus Aspergillus nidulans. Mol. Microbiol. 2003, 47, 1577–1588. [Google Scholar] [PubMed]
  58. Graessle, S.; Dangl, M.; Haas, H.; Mair, K.; Trojer, P.; Brandtner, E.M.; Walton, J.D.; Loidl, P.; Brosch, G. Characterization of two putative histone deacetylase genes from Aspergillus nidulans. Biochim. Biophys. Acta 2000, 1492, 120–126. [Google Scholar] [PubMed]
  59. Aguirre, J.; Rios-Momberg, M.; Hewitt, D.; Hansberg, W. Reactive oxygen species and development in microbial eukaryotes. Trends Microbiol. 2005, 13, 111–118. [Google Scholar] [PubMed]
  60. Furukawa, K.; Yoshimi, A.; Furukawa, T.; Hoshi, Y.; Hagiwara, D.; Sato, N.; Fujioka, T.; Mizutani, O.; Mizuno, T.; Kobayashi, T.; et al. Novel reporter gene expression systems for monitoring activation of the Aspergillus nidulans HOG pathway. Biosci. Biotechnol. Biochem. 2007, 71, 1724–1730. [Google Scholar] [PubMed]
  61. Borgia, P.T.; Miao, Y.; Dodge, C.L. The orlA gene from Aspergillus nidulans encodes a trehalose-6-phosphate phosphatase necessary for normal growth and chitin synthesis at elevated temperatures. Mol. Microbiol. 1996, 20, 1287–1296. [Google Scholar] [PubMed]
  62. d'Enfert, C.; Fontaine, T. Molecular characterization of the Aspergillus nidulans treA gene encoding an acid trehalase required for growth on trehalose. Mol. Microbiol. 1997, 24, 203–216. [Google Scholar] [PubMed]
  63. Fillinger, S.; Chaveroche, M.K.; van Dijck, P.; de Vries, R.; Ruijter, G.; Thevelein, J.; d'Enfert, C. Trehalose is required for the acquisition of tolerance to a variety of stresses in the filamentous fungus Aspergillus nidulans. Microbiology 2001, 147, 1851–1862. [Google Scholar] [PubMed]
  64. Fillinger, S.; Ruijter, G.; Tamas, M.J.; Visser, J.; Thevelein, J.M.; d'Enfert, C. Molecular and physiological characterization of the NAD-dependent glycerol 3-phosphate dehydrogenase in the filamentous fungus Aspergillus nidulans. Mol. Microbiol. 2001, 39, 145–157. [Google Scholar] [PubMed]
  65. de Vries, R.P.; Flitter, S.J.; van de Vondervoort, P.J.; Chaveroche, M.K.; Fontaine, T.; Fillinger, S.; Ruijter, G.J.; d'Enfert, C.; Visser, J. Glycerol dehydrogenase, encoded by gldB is essential for osmotolerance in Aspergillus nidulans. Mol. Microbiol. 2003, 49, 131–141. [Google Scholar] [CrossRef] [PubMed]
  66. Du, C.; Sarfati, J.; Latge, J.P.; Calderone, R. The role of the sakA (Hog1) and tcsB (sln1) genes in the oxidant adaptation of Aspergillus fumigatus. Med. Mycol. 2006, 44, 211–218. [Google Scholar] [PubMed]
  67. Reyes, G.; Romans, A.; Nguyen, C.K.; May, G.S. Novel mitogen-activated protein kinase MpkC of Aspergillus fumigatus is required for utilization of polyalcohol sugars. Eukaryotic Cell 2006, 5, 1934–1940. [Google Scholar] [PubMed]
  68. Xue, T.; Nguyen, C.K.; Romans, A.; May, G.S. A mitogen-activated protein kinase that senses nitrogen regulates conidial germination and growth in Aspergillus fumigatus. Eukaryotic Cell 2004, 3, 557–560. [Google Scholar] [PubMed]
  69. Hagiwara, D.; Mizuno, T.; Abe, K. Characterization of NikA histidine kinase and two response regulators with special reference to osmotic adaptation and asexual development in Aspergillus nidulans. Biosci. Biotechnol. Biochem. 2009, 73, 1566–1571. [Google Scholar] [PubMed]
  70. Hagiwara, D.; Asano, Y.; Marui, J.; Yoshimi, A.; Mizuno, T.; Abe, K. Transcriptional profiling for Aspergillus nidulans HogA MAPK signaling pathway in response to fludioxonil and osmotic stress. Fungal Genet. Biol. 2009, 46, 868–878. [Google Scholar] [PubMed]
  71. Ma, Y.; Qiao, J.; Liu, W.; Wan, Z.; Wang, X.; Calderone, R.; Li, R. The sho1 sensor regulates growth, morphology, and oxidant adaptation in Aspergillus fumigatus but is not essential for development of invasive pulmonary aspergillosis. Infect Immun. 2008, 76, 1695–1701. [Google Scholar] [PubMed]
  72. Alonso-Monge, R.; Navarro-Garcia, F.; Roman, E.; Negredo, A.I.; Eisman, B.; Nombela, C.; Pla, J. The Hog1 mitogen-activated protein kinase is essential in the oxidative stress response and chlamydospore formation in Candida albicans. Eukaryotic Cell 2003, 2, 351–361. [Google Scholar] [PubMed]
  73. Hicks, J.; Lockington, R.A.; Strauss, J.; Dieringer, D.; Kubicek, C.P.; Kelly, J.; Keller, N. RcoA has pleiotropic effects on Aspergillus nidulans cellular development. Mol. Microbiol. 2001, 39, 1482–1493. [Google Scholar] [PubMed]
  74. Todd, R.B.; Hynes, M.J.; Andrianopoulos, A. The Aspergillus nidulans rcoA gene is required for veA-dependent sexual development. Genetics 2006, 174, 1685–1688. [Google Scholar] [PubMed]
  75. Han, K.H.; Seo, J.A.; Yu, J.H. A putative G protein-coupled receptor controls growth, germination and coordinated development in Aspergillus nidulans. Fungal Genet. Newsl. 2003, 50. [Google Scholar]
  76. Mert, H.H.; Ekmekci, S. The effect of salinity and osmotic pressure of the medium on the growth, sporulation and changes in the total organic acid content of Aspergillus flavus and Penicillium chrysogenum. Mycopathologia 1987, 100, 85–89. [Google Scholar] [CrossRef] [PubMed]
  77. Cotty, P.J. Virulence and cultural characteristics of two Aspergillus flavus strains pathogenic on cotton. Phytopathology 1989, 79, 808–814. [Google Scholar]
  78. Calvo, A.M. The VeA regulatory system and its role in morphological and chemical development in fungi. Fungal Genet. Biol. 2008, 45, 1053–1061. [Google Scholar] [PubMed]
  79. El-Abyad, M.S.; Hindorf, H.; Rizk, M.A. Impact of salinity stress on soil-borne fungi of sugarbeet. Plant Soil 1988, 110, 27–32. [Google Scholar]
  80. Ramos, A.J.M.; Magan, N.; Sanchis, V. Osmotic and matric potential effects on growth, sclerotia and partitioning of polyols and sugars in colonies and spores of Aspergillus ochraceus. Mycol. Res. 1999, 103, 141–147. [Google Scholar] [CrossRef]
  81. Whipps, J.M.M.; Magan, N. Effects of nutrient status and water potential of media on fungal growth and antagonist-pathogen interactions. EPPO Bulletin 1986, 17, 581–591. [Google Scholar] [CrossRef]
  82. Calvo, A.M.; Wilson, R.A.; Bok, J.W.; Keller, N.P. Relationship between secondary metabolism and fungal development. Microbiol. Mol. Biol. Rev. 2002, 66, 447–459. [Google Scholar] [PubMed]
  83. Hicks, J.; Lockington, R.A.; Strauss, J.; Dieringer, D.; Kubicek, C.P.; Kelly, J.; Keller, N. RcoA has pleiotropic effects on Aspergillus nidulans cellular development. Mol. Microbiol. 2001, 39, 1482–1493. [Google Scholar] [PubMed]
  84. Ochiai, N.; Tokai, T.; Nishiuchi, T.; Takahashi-Ando, N.; Fujimura, M.; Kimura, M. Involvement of the osmosensor histidine kinase and osmotic stress-activated protein kinases in the regulation of secondary metabolism in Fusarium graminearum. Biochem. Biophys. Res. Commun. 2007, 363, 639–644. [Google Scholar] [PubMed]
  85. Igbaria, A.; Lev, S.; Rose, M.S.; Lee, B.N.; Hadar, R.; Degani, O.; Horwitz, B.A. Distinct and combined roles of the MAP kinases of Cochliobolus heterostrophus in virulence and stress responses. Mol. Plant Microbe Interact. 2008, 21, 769–780. [Google Scholar] [PubMed]
  86. Park, S.M.; Choi, E.S.; Kim, M.J.; Cha, B.J.; Yang, M.S.; Kim, D.H. Characterization of HOG1 homologue, CpMK1, from Cryphonectria parasitica and evidence for hypovirus-mediated perturbation of its phosphorylation in response to hypertonic stress. Mol. Microbiol. 2004, 51, 1267–1277. [Google Scholar] [CrossRef] [PubMed]
  87. Dixon, K.P.; Xu, J.R.; Smirnoff, N.; Talbot, N.J. Independent signaling pathways regulate cellular turgor during hyperosmotic stress and appressorium-mediated plant infection by Magnaporthe grisea. Plant Cell 1999, 11, 2045–2058. [Google Scholar] [PubMed]
  88. Chapmann, R.F. The Insects: Structure and Function; Cambridge University Press: Cambridge, UK, 1998. [Google Scholar]
  89. Wang, C.; Duan, Z.; St Leger, R.J. MOS1 osmosensor of Metarhizium anisopliae is required for adaptation to insect host hemolymph. Eukaryotic Cell 2008, 7, 302–309. [Google Scholar] [PubMed]
  90. Vylkova, S.; Jang, W.S.; Li, W.; Nayyar, N.; Edgerton, M. Histatin 5 initiates osmotic stress response in Candida albicans via activation of the Hog1 mitogen-activated protein kinase pathway. Eukaryotic Cell 2007, 6, 1876–1888. [Google Scholar] [PubMed]

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Duran, R.; Cary, J.W.; Calvo, A.M. Role of the Osmotic Stress Regulatory Pathway in Morphogenesis and Secondary Metabolism in Filamentous Fungi. Toxins 2010, 2, 367-381. https://doi.org/10.3390/toxins2040367

AMA Style

Duran R, Cary JW, Calvo AM. Role of the Osmotic Stress Regulatory Pathway in Morphogenesis and Secondary Metabolism in Filamentous Fungi. Toxins. 2010; 2(4):367-381. https://doi.org/10.3390/toxins2040367

Chicago/Turabian Style

Duran, Rocio, Jeffrey W. Cary, and Ana M. Calvo. 2010. "Role of the Osmotic Stress Regulatory Pathway in Morphogenesis and Secondary Metabolism in Filamentous Fungi" Toxins 2, no. 4: 367-381. https://doi.org/10.3390/toxins2040367

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