Protein profiling of post-prostatic massage urine specimens by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry to discriminate between prostate cancer and benign lesions

  • Authors:
    • Akiko Okamoto
    • Hayato Yamamoto
    • Atsushi Imai
    • Shingo Hatakeyama
    • Ikuya Iwabuchi
    • Takahiro Yoneyama
    • Yasuhiro Hashimoto
    • Takuya Koie
    • Noritaka Kamimura
    • Kazuyuki Mori
    • Kanemitsu Yamaya
    • Chikara Ohyama
  • View Affiliations

  • Published online on: January 1, 2009     https://doi.org/10.3892/or_00000191
  • Pages: 73-79
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Abstract

Post-prostatic massage urine specimens (PMUS) are expected to be rich in proteins originating from the prostatic acini. In this study, we created a PMUS bank consisting of 57 samples obtained from patients with biopsy-proven prostate cancer (PC) and 56 samples from subjects with biopsy-proven benign lesions to analyze protein profiles of PMUS by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). Strong anion-exchange (Q10), weak cation-exchange (CM10) and immobilized metal affinity capture (IMAC30) ProteinChip Arrays were used for protein profiling. In PC samples, single-marker analysis detected 49 mass peaks that were significantly up-regulated and 23 peaks that were significantly down-regulated, compared with peaks obtained from benign lesion samples. To confirm reproducibility we performed additional three rounds of assay using CM10 chip with pH 4.0 binding buffer. Among these significant peaks, a peak of m/z 10788 was significant throughout all 4 rounds of assays. For hierarchical clustering analysis (HCA), we used the 72 peaks which revealed significant differences in single-marker analysis. The heat map discriminated PC from benign lesions with a sensitivity of 91.7% and a specificity of 83.3%. Therefore, SELDI-TOF MS profiling of PMUS can be applied to differentiate patients with PC from cancer-free subjects. However, further investigation is required to verify the usefulness of this method in clinical practice.

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January 2009
Volume 21 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Okamoto A, Yamamoto H, Imai A, Hatakeyama S, Iwabuchi I, Yoneyama T, Hashimoto Y, Koie T, Kamimura N, Mori K, Mori K, et al: Protein profiling of post-prostatic massage urine specimens by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry to discriminate between prostate cancer and benign lesions. Oncol Rep 21: 73-79, 2009
APA
Okamoto, A., Yamamoto, H., Imai, A., Hatakeyama, S., Iwabuchi, I., Yoneyama, T. ... Ohyama, C. (2009). Protein profiling of post-prostatic massage urine specimens by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry to discriminate between prostate cancer and benign lesions. Oncology Reports, 21, 73-79. https://doi.org/10.3892/or_00000191
MLA
Okamoto, A., Yamamoto, H., Imai, A., Hatakeyama, S., Iwabuchi, I., Yoneyama, T., Hashimoto, Y., Koie, T., Kamimura, N., Mori, K., Yamaya, K., Ohyama, C."Protein profiling of post-prostatic massage urine specimens by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry to discriminate between prostate cancer and benign lesions". Oncology Reports 21.1 (2009): 73-79.
Chicago
Okamoto, A., Yamamoto, H., Imai, A., Hatakeyama, S., Iwabuchi, I., Yoneyama, T., Hashimoto, Y., Koie, T., Kamimura, N., Mori, K., Yamaya, K., Ohyama, C."Protein profiling of post-prostatic massage urine specimens by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry to discriminate between prostate cancer and benign lesions". Oncology Reports 21, no. 1 (2009): 73-79. https://doi.org/10.3892/or_00000191