Brief Article Open Access
Copyright ©2012 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Hepatol. May 27, 2012; 4(5): 169-175
Published online May 27, 2012. doi: 10.4254/wjh.v4.i5.169
Global hypomethylation in hepatocellular carcinoma and its relationship to aflatoxin B1 exposure
Yu-Jing Zhang, Hui-Chen Wu, Regina M Santella, Department of Environmental Health Sciences, Mailman School of Public Health of Columbia University, 630 W 168Th St., New York, NY 10032, United States
Hulya Yazici, Department of Oncology, Oncology Institute, Istanbul University, Istanbul 34093, Turkey
Ming-Whei Yu, Graduate Institute of Epidemiology, College of Public Health, National Taiwan University, Taipei 10002, Taiwan, China
Po-Huang Lee, Department of Surgery, College of Medicine, National Taiwan University, Taipei 10002, Taiwan, China
Author contributions: Zhang YJ and Santella RM designed the research; Zhang YJ and Yazici H performed the assays; Wu HC did the statistical analysis; Yu MW and Lee PH supplied paired hepatocellular carcinoma samples and clinical data; and Zhang YJ and Santella RM wrote the paper.
Supported by A grant from the National Institute of Health, No. ES005116 and No. P30ES009089
Correspondence to: Yu-Jing Zhang, MD, Senior Research Scientist, Department of Environmental Health Sciences, Mailman School of Public Health of Columbia University, 630 W 168Th St., New York, NY 10032, United States. yz6@columbia.edu
Telephone: +1-212-3058158 Fax: +1-212-3055328
Received: November 19, 2011
Revised: February 2, 2012
Accepted: April 27, 2012
Published online: May 27, 2012

Abstract

AIM: To determine global DNA methylation in paired hepatocellular carcinoma (HCC) samples using several different assays and explore the correlations between hypomethylation and clinical parameters and biomarkers, including that of aflatoxin B1 exposure.

METHODS: Using the radio labeled methyl acceptance assay as a measure of global hypomethylation, as well as two repetitive elements, including satellite 2 (Sat2) by MethyLight and long interspersed nucleotide elements (LINE1), by pyrosequencing.

RESULTS: By all three assays, mean methylation levels in tumor tissues were significantly lower than that in adjacent tissues. Methyl acceptance assay log (mean ± SD) disintegrations/min/ng DNA are 70.0 ± 54.8 and 32.4 ± 15.6, respectively, P = 0.040; percent methylation of Sat2 42.2 ± 55.1 and 117.9 ± 88.8, respectively, P < 0.0001 and percent methylation LINE1 48.6 ± 14.8 and 71.7 ± 1.4, respectively, P < 0.0001. Aflatoxin B1-albumin (AFB1-Alb) adducts, a measure of exposure to this dietary carcinogen, were inversely correlated with LINE1 methylation (r = -0.36, P = 0.034).

CONCLUSION: Consistent hypomethylation in tumor compared to adjacent tissue was found by the three different methods. AFB1 exposure is associated with DNA global hypomethylation, suggesting that chemical carcinogens may influence epigenetic changes in humans.

Key Words: Hepatocellular carcinoma, Epigenetics, Hypomethylation, [3H]-methyl acceptance assay, Satellite 2, Long interspersed nucleotide element-1, Aflatoxin B1



INTRODUCTION

Hepatocellular carcinoma (HCC) is one of the most common cancers in the world and a leading cause of death worldwide, especially in Saharan Africa and southern Asia, including Taiwan, Thailand, Hong Kong and southern China[1,2]; it is also increasing in Western, developed countries such as the United States[3]. HCC incidence is associated with various risk factors, including chronic hepatitis B virus (HBV) and hepatitis C virus (HCV) infections, alcohol consumption and several environmental factors, especially aflatoxin B1 (AFB1), a dietary mold contaminant, and polycyclic aromatic hydrocarbons (PAHs), ubiquitous environmental contaminants[4-6].

As with other cancers, the development of HCC is a complex, multistep process, involving multiple genetic aberrations in the molecular control of hepatocyte proliferation, differentiation and death and the maintenance of genomic integrity. This process is influenced by the cumulative activation and inactivation of oncogenes, tumor suppressor genes and other genes[7,8]. Epigenetic alterations are also involved in cancer development and progression[9,10]. Human tumors often display changes in DNA methylation, including both gene-specific promoter hypermethylation and genome-wide hypomethylation[11,12]. Frequent promoter hypermethylation and subsequent loss of protein expression of tumor suppressor genes has been demonstrated in HCC[13]. Global hypomethylation, in both noncoding repetitive sequences and in genes, contributes to carcinogenesis by causing chromosome instability, reactivation of transposable elements, loss of imprinting and increased gene expression, and has been detected in different human cancer tissues, including HCC[14].

Hypomethylation of the genome mainly affects the intergenic and intronic regions of DNA, particularly repeat sequences and transposable elements[15]. Repetitive elements, which consist of interspersed and tandem repeats, comprise about 45% of the human genome[16,17]. More than 90% of all 5-methylcytosine (5mC) lies within the transposons, including short interspersed nucleotide elements and long interspersed nucleotide elements (LINEs), which are comparatively rich in CpG dinucleotides[14]. Satellite 2 (Sat2) DNA sequences are located as tandem repeats in the pericentromeric and juxtacentromeric heterochromatin of several chromosomes[18]. Loss of DNA methylation in these sequences is believed to mainly account for global hypomethylation[19]. Analysis of methylation levels of Sat2 and LINE1 are frequently used as a measure of global methylation since levels measured using the MethyLight assay were significantly associated with methylation, as measured by high-performance liquid chromatography quantitation of 5mC[20].

The methyl group acceptance assay also can be used to determine global DNA methylation levels. It is based on the ability of isolated DNA to “accept” radio labeled methyl groups from S-[3H-methyl] adenosylmethionine, using the bacterial CpG methyltransferase SssI. As this enzyme methylates all unmethylated CpG dinucleotides in the genome, radio labeled methyl group acceptance is inversely proportional to the level of preexisting methylation[21].

In the current study, global DNA methylation status in paired HCC and their adjacent non-tumor tissues was measured using the methyl acceptance assay, analysis of Sat2 by MethyLight and LINE1 by pyrosequencing. Data were correlated to both clinical data and other available biomarker data on exposure to AFB1 and gene-specific promoter methylation.

MATERIALS AND METHODS
Patient population and data on clinical parameters

The study samples consisted of frozen dissected tumor and adjacent tissues from HCC patients, collected in the Department of Surgery, National Taiwan University Hospital. Informed consent was obtained from patients and the study was approved by the appropriate institutional review committees. Data on demographics and clinicopathological characteristics obtained from hospital charts, and HBV and HCV status, determined by immunoassay, were published previously[22]. Plasma collected at the time of surgery had been previously analyzed for the albumin adducts of AFB1. In addition, methylation of p16Ink4A and HINT1 were previously determined in the tumor tissues by methylation specific PCR[22-23].

DNA extraction

DNA was isolated from frozen tissue samples, as previously described[24]. Briefly, tissue was placed in liquid nitrogen and pulverized with a blender. The tissue powder was lysed with a DNA lysing buffer (10 mmol/L Tris, 10 mmol/L NaCl, 0.1% sodium dodecyl sulfate at pH 7.9 and 200 μg/mL proteinase K). DNA was isolated by RNase treatment, phenol/chloroform extraction and ethanol precipitation. The laboratory investigator who performed the assays was blinded to epidemiological data.

Sat2 MethyLight assay

After sodium bisulfite conversion (EZ DNA methylation kit, Zymo Research, Orange, CA), genomic DNA was amplified using the previously reported Sat2 M1 and Alu C4 (control for DNA input) primers and probes[20]. Bisulfite-converted, CpGenome universal methylated DNA (Chemical International, Temecula, CA) served as the methylated reference. A pooled sample of DNA from 5 controls was used as a quality control and analyzed with each batch of test samples. All samples were analyzed in duplicate on an ABI Prism 7900 Sequence Detection System (Perkin-Elmer, Foster City, CA). Intra- and inter-assay coefficients of variation (CVs) were 1.2 and 1.9, respectively. The data are expressed as a percentage of methylated reference (PMR) values.

PMR = 100% * 2 exp - {Delta Ct (target gene in sample - control gene in sample) - Delta Ct (target gene in fully methylated reference sample - control gene in reference sample)}.

LINE1 amplification and pyrosequencing

The assay for LINE1 was carried out essentially as described previously, using reported primer and sequencing probe sequences as well as PCR conditions[25]. We used non-CpG cytosine residues as internal controls to verify efficient sodium bisulfate DNA conversion and controls were as in the MethyLight assay. Pyrosequencing was conducted using a PyroMark Q24 instrument (Qiagen), with subsequent quantitation of methylation levels determined with the PyroMark Q24 1.010 software. Relative peak height differences were used to calculate the percentage of methylated cytosines at each given site. Percent methylation within a sample was subsequently determined by averaging across all three interrogated CpG sites in the analysis. The inter-assay CV was 1.1.

[3H]-Methyl acceptance assay

The [3H]-methyl acceptance assay was carried out as described by Balaghi and Wagner[26] and Pilsner et al[27]. The DNA was incubated with [3H]S-adenosylmethionine in the presence of the SssI prokaryotic methylase enzyme. Briefly, 200 ng of DNA was incubated with 3 U of SssI methylase (New England Biolabs); 3.8 μmol/L (1.1 μCi) [3H]-labeled S-adenosylmethionine (Perklin-Elmer); and EDTA, DTT, and Tris-HCL (pH 8.2) in a 30 μL mixture and incubated for 1 h at 37 °C. The reaction was terminated on ice and 15 μL of the reaction mixture applied onto Whatman DE81 filter paper. The filter was washed on a vacuum filtration apparatus three times with 5 mL of 0.5 mol/L sodium phosphate buffer (pH 8.0), followed by 2 mL each of 70% and 100% ethanol. Dried filters were each placed in a vial with 5 mL of scintillation fluid (Scintisafe, Thermo Fisher, Waltham, MA) and analyzed by a Packard scintillation counter to determine counts/min then converted to disintegrations/min (DPM) based on counting efficiency. Each DNA sample was processed in duplicate and each processing run included samples for background (reaction mixture with all components except SssI enzyme) and controls as for the other assays. Intra- and inter-assay CVs were 2.0 and 3.9, respectively. DPM values were expressed per ng DNA as quantified by PicoGreen using double-strand DNA quantification reagent (Molecular Probes, Life Technologies, Grand Island, NY).

Statistical analysis

Paired t-test was used to examine differences in methylation levels between tumor and adjacent tissues after natural log-transformation to normalize the distribution. We present the values as arithmetic data for ease of interpretation. Spearman correlation coefficients were used to determine the correlation between methylation and AFB1-Alb adducts. Wilcoxon signed-rank test was used to compare methylation levels and clinical characteristics. All analyses were performed with SAS software 9.0 (SAS Institute, Cary, NC). All statistical tests were based on two-tailed probability.

RESULTS

Methylation levels of DNA from HCC and adjacent non-tumor tissues were determined by the methyl acceptance assay, a measure of global methylation. Two repetitive elements were also analyzed as an additional measure of methylation, including Sat2 by MethyLight and LINE1 by pyrosequencing. For all three assays, mean methylation level was significantly lower in tumor compared to adjacent non-tumor tissues. For the methyl acceptance assay, mean levels of DPM/ng DNA were 70.0 ± 54.8 and 32.4 ± 15.6, respectively (P = 0.040); for Sat2 by the MethyLight assay, values were 42.2% ± 55.1% and 117.9% ± 88.8% (P < 0.0001); and for LINE1, 48.6 ± 14.8 and 71.7 ± 1.4% (P < 0.0001), respectively (Table 1).

Table 1 Methylation levels of hepatocellular carcinoma tumor and adjacent non-tumor liver tissue.
TumorAdjacentP value
mean ± SDmean ± SD
LINE1 (%)48.6 (14.8)71.7 (1.4)< 0.0001
Sat2 (%)42.2 (55.1)117.9 (88.8)< 0.0001
Methyl acceptance (DPM/ng)70.0 (54.8)32.4 (15.6)0.040

For the methyl acceptance assay, in 28 of 37 paired samples (75.7%), methylation in tumor tissues was lower than that in adjacent non-tumor tissues. For Sat2 and LINE1 analysis, in 31 (83.8%) and 32 (86.5%) subjects, levels were lower in tumor than in adjacent non-tumor tissues, respectively.

Plasma levels of AFB1-Alb adducts had been measured previously in bloods collected at the time of surgery[22]. As hypothesized, plasma levels of AFB1-Alb adducts were statistically significantly inversely correlated with methylation levels of LINE1 (r = -0.36, P = 0.034) (Table 2 and Figure 1). Plasma levels of AFB1-Alb adducts were also inversely correlated with tumor methylation levels measured by Sat2, but not statistically significantly (r = -0.30, P = 0.082, Table 2). Since higher values in the methyl acceptance assay indicate hypomethylation, the correlation between adducts and methylation in this assay is also in the correct direction but not significant (r = 0.18, P = 0.286, Table 2).

Figure 1
Figure 1 The correlation between aflatoxin B1-Alb levels in plasma (aflatoxin B1/µg) and long interspersed nucleotide element-1methylation (%) in tumor tissue. AFB1: Aflatoxin B1; LINE1: Long interspersed nucleotide element-1.
Table 2 Correlations between methylation levels and aflatoxin B1- albumin adducts in hepatocellular carcinoma tumor tissues.
AFB1-AlbP value
r
LINE1 (%)-0.360.034
Sat2 (%)-0.300.082
Methylacceptance DPM/ng)0.180.286

The associations of HBV and HCV infection status, cirrhosis status and promoter hypermethylation of p16Ink4A and Hint1 with global hypomethylation in tumor tissue are given in Table 3. No statistically significantly correlations were found, except for LINE1 and being positive for both HBV and HCV infection. However, only one subject was negative for both HBV and HCV so this result is likely to be spurious.

Table 3 Methylation levels in tumor tissues and clinical characteristic and gene-specific methylation in tumor tissues.
VariablenLINE1 (%)P valueSat2 (%)P valueMethyl acceptance (DPM/ng)P value
mean ± SDmean ± SDmean ± SD
HBsAg
Negative541.0 (16.6)0.24522.1 (19.2)0.90567.2 (37.6)0.607
Positive2647.7 (13.6)31.3 (34.5)81.3 (58.3)
AntiHCV
Negative1849.6 (14.2)0.44927.6 (22.6)0.73772.1 (49.7)0.759
Positive1045.2 (14.3)38.0 (47.7)86.5 (71.3)
HBsAg and AntiHCV
Both negative124.90.0475.70.32364.50.877
Either one positive3147.2 (13.5)31.1 (32.2)78.4 (55.5)
Cirrhosis
No1744.8 (15.2)0.38828.1 (23.8)0.79178.6 (52.4)0.927
Yes1348.9 (13.2)32.5 (43.1)82.3 (61.9)
p16Ink4A
Unmethylated1550.9 (16.7)0.56963.4 (80.8)0.43762.0 (61.2)0.155
Methylated2247.1 (13.5)27.8 (21.1)75.4 (50.8)
Hint1
Unmethylated1750.9 (14.8)0.40551.8 (75.8)0.95160.2 (48.9)0.142
Methylated2046.7 (14.8)34.1 (30.3)78.3 (59.4)
DISCUSSION

Hypomethylation was observed in tumor compared to adjacent non-tumor tissues using three different assays that measure global methylation or methylation in two repetitive elements. The level of [3H]-methyl acceptance of HCC tumor DNAs was statistically significantly higher compared to that of adjacent non-tumor DNAs (P < 0.040), indicating significantly lower methylation. This is the first study to report that global hypomethylation contributes to hepatocarcinogenesis using the [3H]-methyl acceptance assay. Global loss of methylation in cancer may lead to alterations in the expression of proto oncogenes critical to carcinogenesis and facilitate chromosomal instability[28].

Repetitive DNA elements are normally heavily methylated and a previous study showed a correlation between Alu, Sat2 and LINE1 methylation by MethyLight and 5mC content in normal and tumor samples[20], indicating the usefulness of these assays as surrogate measures of genomic methylation levels. In this study, tumor methylation was statistically significantly lower than in paired adjacent non-tumor tissue for Sat2 (P < 0.0001) by the MethyLight assay and for LINE1 (P < 0.0001) by pyrosequencing, consistent with the data from the methyl acceptance assay and as reported previously[14,27,28].

A previous study found that three repetitive DNA elements, Sat2, Alu and LINE1, showed discordance in timing of hypomethylation along the multistep pathway in hepatocarcinogenesis from normal liver to HCC; Sat2 hypomethylation occurred at the chronic hepatitis stage[29]. Hypomethylation also differed according to geographic location of the subjects and their hepatitis infection status; mean LINE1 methylation in tumor samples was lower in hepatitis-positive cases than in hepatitis-negative cases[30]. These findings suggest that HBV or HCV infection can influence global DNA hypomethylation status. This may be partially explained by the fact that the HBV X protein can induce altered DNA methyltransferase activity, hypermethylation of specific CpG islands and global hypomethylation[30,31]. In the present study, no associations between DNA global hypomethylation and HBV and HCV infection were observed (Table 3). However, only one case was negative for markers of infection for either HBV or HCV, limiting our ability to investigate the role of infection on methylation levels.

Exposure to AFB1 is one of the major risk factors for the development of HCC. In our previous studies, we found a strong relationship between AFB1 exposure and promoter hypermethylation in tumor suppressor and other cancer-related genes, including RASSF1A[32], p16 Ink4A[22,32] and MGMT[33] in tumor tissues and plasma DNA of HCC patients. AFB1 may bind preferentially to methylated CpG sites and/or specific structures in chromatin, inducing damage to DNA and histones[33] that may impact on methylation. Several other environmental exposures have been associated with epigenetic changes. Increasing air levels of benzene, a chemical carcinogen, was associated with a significant reduction in LINE1 and Alu1 methylation in white blood cells[34]. LINE1 DNA methylation is also inversely associated with lead exposure in humans[35]. Even although the mechanisms are still not clear, these data suggest that exposure to some chemical carcinogens may cause changes in global methylation status. In the present study, plasma levels of AFB1-Alb adducts were statistically significantly inversely correlated with methylation levels of LINE1, providing additional evidence that carcinogens may alter global methylation. Reactive oxygen species and the resulting DNA damage produced by AFB1 may reduce binding affinity of methyl-CpG binding protein 2, therefore resulting in epigenetic alterations[36,37].

It is still uncertain whether or not gene-specific promoter hypermethylation and global hypomethylation are independent processes; in HCC, their correlation is still controversial. One recent study demonstrated that global hypomethylation in HCC was associated with gene-specific hypermethylation[30], but another showed variability between individual CpG islands’ hypermethylation and repetitive DNA hypomethylation status and concluded that there is no mechanistic link in liver cancer cells[29]. We also found no association between promoter hypermethylation in the two specific genes investigated and global hypomethylation in HCC tissue DNAs. In addition, gene-specific hypermethylation and global hypomethylation appear to be independent processes in colon and urothelial cancers[38,39]. Further investigations are still needed to validate the relationship between global hypomethylation and gene-specific promoter hypermethylation.

In summary, this is the first study to investigate global hypomethylation, one of the most consistent epigenetic changes in cancer development in HCC, and paired adjacent non-tumor tissues using three different methods: the methyl acceptance assay and analysis of Sat2 and LINE1, two repetitive elements. Consistent hypomethylation in tumor compared to adjacent tissue was found by all three methods. AFB1 exposure was also associated with DNA hypomethylation, suggesting that chemical carcinogens may influence epigenetic changes in human tissues.

COMMENTS
Background

Epigenetic alterations are involved in cancer development and progression. Promoter CpG island hypermethylation contributes to carcinogenesis by shutting off expression of tumor suppressor and DNA repair genes. Genomic DNA hypomethylation is implicated in carcinogenesis by inducing chromosome instability and loss of imprinting. Genome-wide hypomethylation has been reported in a variety of cancers, including hepatocellular carcinoma (HCC). Hypomethylation of the genome mainly affects the intergenic and intronic regions of DNA, particularly repeat sequences and transposable elements. Analysis of methylation levels of Satellite 2 (Sat2) and long interspersed nucleotide element-1 (LINE1) is frequently used as a measure of global methylation. The correlations between global hypomethylation and hepatitis infection status have been investigated, but the association between hypomethylation and aflatoxin B1 (AFB1) exposure in HCC is still unclear.

Research frontiers

Genomic DNA hypomethylation is a common finding in human cancers. Global DNA hypomethylation reflected in reduced levels of methylation in repeat regions, occurs in target tissues undergoing carcinogenic differentiation, and could be used as a biomarker of malignant tumors. Environmental factors such as geographic location and hepatitis status have been shown to contribute to hepatocarcinogenesis through global hypomethylation.

Innovations and breakthroughs

In the present study, the authors first investigated DNA methylation in HCC and paired adjacent non-tumor tissues using the methyl acceptance assay as a measure of global methylation. They also analyzed two repetitive elements, including Sat2 by MethyLight and LINE1 by pyrosequencing. With all three assays, mean methylation levels in tumor tissues were significantly lower than that in adjacent non-tumor tissues. They also first found that AFB1-albumin adducts levels were inversely correlated with LINE1 methylation, providing an additional mechanism by which exposure to this dietary carcinogen may influence hepatocarcinogenesis.

Applications

This work demonstrated that methyl acceptance assay could be used to accurately detect global hypomethylation in HCC samples. Finding that AFB1 exposure is correlated with global hypomethylation, as well as hypermethylation in some genes, demonstrates the important role it plays in the development of HCC. This may help to develop new strategies to prevent HCC.

Terminology

Global hypomethylation is a decrease in the overall genomic 5-methylcytosine content (compared to total cytosines) from approximately 4% in normal tissues to 2%-3% in cancer tissues. This change was first observed in a number of studies in 1983, in lung and colon carcinomas compared to adjacent normal tissue, and in various malignancies compared to various postnatal tissues, demonstrating that overall genomic 5-methylcytosine levels were lower in cancer tissues. This observation has been reproducibly repeated in a wide range of cancers and matched normal tissues using a variety of different techniques.

Peer review

This manuscript addresses an interesting issue for the initiation and progression of HCC.

Footnotes

Peer reviewers: Fei Chen, Associate Professor, Wayne State University, Detroit, MI 48201, United States; Dr. Drazen B Zimonjic, Laboratory of Experimental Carcinogenesis, National Cancer Institute, 37 Convent Dr., MSC 4262, ding 37, Room 4128C, Bethesda, MD 20892-4262, United States

S- Editor Wu X L- Editor Roemmele A E- Editor Wu X

References
1.  Beasley RP, Lin CC, Chien CS, Chen CJ, Hwang LY. Geographic distribution of HBsAg carriers in China. Hepatology. 1982;2:553-556.  [PubMed]  [DOI]  [Cited in This Article: ]
2.  Munoz N, Bosch X. Epidemiology of Hepatocellular Carcinoma. Neoplasms of the Liver. Tokyo: Springer 1989; 3.  [PubMed]  [DOI]  [Cited in This Article: ]
3.  El-Serag HB, Mason AC. Rising incidence of hepatocellular carcinoma in the United States. N Engl J Med. 1999;340:745-750.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 2221]  [Cited by in F6Publishing: 2124]  [Article Influence: 85.0]  [Reference Citation Analysis (0)]
4.  Chen CJ, Yu MW, Liaw YF. Epidemiological characteristics and risk factors of hepatocellular carcinoma. J Gastroenterol Hepatol. 1997;12:S294-S308.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 341]  [Cited by in F6Publishing: 359]  [Article Influence: 13.3]  [Reference Citation Analysis (0)]
5.  Chen SY, Wang LY, Lunn RM, Tsai WY, Lee PH, Lee CS, Ahsan H, Zhang YJ, Chen CJ, Santella RM. Polycyclic aromatic hydrocarbon-DNA adducts in liver tissues of hepatocellular carcinoma patients and controls. Int J Cancer. 2002;99:14-21.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 82]  [Cited by in F6Publishing: 91]  [Article Influence: 4.1]  [Reference Citation Analysis (0)]
6.  Santella RM, Zhang YJ, Hsieh LL, Young TL, Lu XQ, Lee BM, Yang GY, Perera FP. Immunological methods for monitoring human expsoure to benzo[a]pyrene and aflatoxin B1: measurement of carcinogen adducts. Immunoassays for Monitoring human exposure to Toxic Chemicals. Washington, DC: ACS Publications 1991; 229-245.  [PubMed]  [DOI]  [Cited in This Article: ]
7.  Herath NI, Leggett BA, MacDonald GA. Review of genetic and epigenetic alterations in hepatocarcinogenesis. J Gastroenterol Hepatol. 2006;21:15-21.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 116]  [Cited by in F6Publishing: 129]  [Article Influence: 7.2]  [Reference Citation Analysis (0)]
8.  Thorgeirsson SS, Grisham JW. Molecular pathogenesis of human hepatocellular carcinoma. Nat Genet. 2002;31:339-346.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 1097]  [Cited by in F6Publishing: 1075]  [Article Influence: 48.9]  [Reference Citation Analysis (0)]
9.  Baylin SB, Herman JG. DNA hypermethylation in tumorigenesis: epigenetics joins genetics. Trends Genet. 2000;16:168-174.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 1168]  [Cited by in F6Publishing: 1145]  [Article Influence: 47.7]  [Reference Citation Analysis (0)]
10.  Jones PA, Laird PW. Cancer epigenetics comes of age. Nat Genet. 1999;21:163-167.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 1657]  [Cited by in F6Publishing: 1588]  [Article Influence: 63.5]  [Reference Citation Analysis (0)]
11.  Feinberg AP, Gehrke CW, Kuo KC, Ehrlich M. Reduced genomic 5-methylcytosine content in human colonic neoplasia. Cancer Res. 1988;48:1159-1161.  [PubMed]  [DOI]  [Cited in This Article: ]
12.  Jones PA, Baylin SB. The fundamental role of epigenetic events in cancer. Nat Rev Genet. 2002;3:415-428.  [PubMed]  [DOI]  [Cited in This Article: ]
13.  Calvisi DF, Ladu S, Gorden A, Farina M, Lee JS, Conner EA, Schroeder I, Factor VM, Thorgeirsson SS. Mechanistic and prognostic significance of aberrant methylation in the molecular pathogenesis of human hepatocellular carcinoma. J Clin Invest. 2007;117:2713-2722.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 293]  [Cited by in F6Publishing: 305]  [Article Influence: 17.9]  [Reference Citation Analysis (0)]
14.  Lin CH, Hsieh SY, Sheen IS, Lee WC, Chen TC, Shyu WC, Liaw YF. Genome-wide hypomethylation in hepatocellular carcinogenesis. Cancer Res. 2001;61:4238-4243.  [PubMed]  [DOI]  [Cited in This Article: ]
15.  Wilson AS, Power BE, Molloy PL. DNA hypomethylation and human diseases. Biochim Biophys Acta. 2007;1775:138-162.  [PubMed]  [DOI]  [Cited in This Article: ]
16.  Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, Baldwin J, Devon K, Dewar K, Doyle M, FitzHugh W. Initial sequencing and analysis of the human genome. Nature. 2001;409:860-921.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 16054]  [Cited by in F6Publishing: 14449]  [Article Influence: 628.2]  [Reference Citation Analysis (0)]
17.  Jordan IK, Rogozin IB, Glazko GV, Koonin EV. Origin of a substantial fraction of human regulatory sequences from transposable elements. Trends Genet. 2003;19:68-72.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 413]  [Cited by in F6Publishing: 408]  [Article Influence: 19.4]  [Reference Citation Analysis (0)]
18.  Jeanpierre M. Human satellites 2 and 3. Ann Genet. 1994;37:163-171.  [PubMed]  [DOI]  [Cited in This Article: ]
19.  Ehrlich M. DNA methylation in cancer: too much, but also too little. Oncogene. 2002;21:5400-5413.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 1124]  [Cited by in F6Publishing: 1052]  [Article Influence: 47.8]  [Reference Citation Analysis (0)]
20.  Weisenberger DJ, Campan M, Long TI, Kim M, Woods C, Fiala E, Ehrlich M, Laird PW. Analysis of repetitive element DNA methylation by MethyLight. Nucleic Acids Res. 2005;33:6823-6836.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 543]  [Cited by in F6Publishing: 551]  [Article Influence: 29.0]  [Reference Citation Analysis (0)]
21.  Nephew KP, Balch C, Skalnik DG. Methyl group acceptance assay for the determination of global DNA methylation levels. Methods Mol Biol. 2009;507:35-41.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 11]  [Cited by in F6Publishing: 13]  [Article Influence: 0.9]  [Reference Citation Analysis (0)]
22.  Zhang YJ, Rossner P, Chen Y, Agrawal M, Wang Q, Wang L, Ahsan H, Yu MW, Lee PH, Santella RM. Aflatoxin B1 and polycyclic aromatic hydrocarbon adducts, p53 mutations and p16 methylation in liver tissue and plasma of hepatocellular carcinoma patients. Int J Cancer. 2006;119:985-991.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 62]  [Cited by in F6Publishing: 69]  [Article Influence: 3.8]  [Reference Citation Analysis (0)]
23.  Zhang YJ, Li H, Wu HC, Shen J, Wang L, Yu MW, Lee PH, Bernard Weinstein I, Santella RM. Silencing of Hint1, a novel tumor suppressor gene, by promoter hypermethylation in hepatocellular carcinoma. Cancer Lett. 2009;275:277-284.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 35]  [Cited by in F6Publishing: 37]  [Article Influence: 2.3]  [Reference Citation Analysis (0)]
24.  Jiang W, Zhang YJ, Kahn SM, Hollstein MC, Santella RM, Lu SH, Harris CC, Montesano R, Weinstein IB. Altered expression of the cyclin D1 and retinoblastoma genes in human esophageal cancer. Proc Natl Acad Sci U S A. 1993;90:9026-9030.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 217]  [Cited by in F6Publishing: 247]  [Article Influence: 8.0]  [Reference Citation Analysis (0)]
25.  Bollati V, Baccarelli A, Hou L, Bonzini M, Fustinoni S, Cavallo D, Byun HM, Jiang J, Marinelli B, Pesatori AC. Changes in DNA methylation patterns in subjects exposed to low-dose benzene. Cancer Res. 2007;67:876-880.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 468]  [Cited by in F6Publishing: 493]  [Article Influence: 29.0]  [Reference Citation Analysis (0)]
26.  Balaghi M, Wagner C. DNA methylation in folate deficiency: use of CpG methylase. Biochem Biophys Res Commun. 1993;193:1184-1190.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 198]  [Cited by in F6Publishing: 206]  [Article Influence: 6.6]  [Reference Citation Analysis (0)]
27.  Pilsner JR, Liu X, Ahsan H, Ilievski V, Slavkovich V, Levy D, Factor-Litvak P, Graziano JH, Gamble MV. Genomic methylation of peripheral blood leukocyte DNA: influences of arsenic and folate in Bangladeshi adults. Am J Clin Nutr. 2007;86:1179-1186.  [PubMed]  [DOI]  [Cited in This Article: ]
28.  Choi IS, Estecio MR, Nagano Y, Kim do H, White JA, Yao JC, Issa JP, Rashid A. Hypomethylation of LINE-1 and Alu in well-differentiated neuroendocrine tumors (pancreatic endocrine tumors and carcinoid tumors). Mod Pathol. 2007;20:802-810.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 109]  [Cited by in F6Publishing: 118]  [Article Influence: 6.9]  [Reference Citation Analysis (0)]
29.  Lee HS, Kim BH, Cho NY, Yoo EJ, Choi M, Shin SH, Jang JJ, Suh KS, Kim YS, Kang GH. Prognostic implications of and relationship between CpG island hypermethylation and repetitive DNA hypomethylation in hepatocellular carcinoma. Clin Cancer Res. 2009;15:812-820.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 72]  [Cited by in F6Publishing: 80]  [Article Influence: 5.3]  [Reference Citation Analysis (0)]
30.  Kim MJ, White-Cross JA, Shen L, Issa JP, Rashid A. Hypomethylation of long interspersed nuclear element-1 in hepatocellular carcinomas. Mod Pathol. 2009;22:442-449.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 34]  [Cited by in F6Publishing: 36]  [Article Influence: 2.4]  [Reference Citation Analysis (0)]
31.  Park IY, Sohn BH, Yu E, Suh DJ, Chung YH, Lee JH, Surzycki SJ, Lee YI. Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein. Gastroenterology. 2007;132:1476-1494.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 225]  [Cited by in F6Publishing: 225]  [Article Influence: 13.2]  [Reference Citation Analysis (0)]
32.  Zhang YJ, Ahsan H, Chen Y, Lunn RM, Wang LY, Chen SY, Lee PH, Chen CJ, Santella RM. High frequency of promoter hypermethylation of RASSF1A and p16 and its relationship to aflatoxin B1-DNA adduct levels in human hepatocellular carcinoma. Mol Carcinog. 2002;35:85-92.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 97]  [Cited by in F6Publishing: 104]  [Article Influence: 4.7]  [Reference Citation Analysis (0)]
33.  Zhang YJ, Chen Y, Ahsan H, Lunn RM, Lee PH, Chen CJ, Santella RM. Inactivation of the DNA repair gene O6-methylguanine-DNA methyltransferase by promoter hypermethylation and its relationship to aflatoxin B1-DNA adducts and p53 mutation in hepatocellular carcinoma. Int J Cancer. 2003;103:440-444.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 67]  [Cited by in F6Publishing: 69]  [Article Influence: 3.3]  [Reference Citation Analysis (0)]
34.  Herceg Z. Epigenetics and cancer: towards an evaluation of the impact of environmental and dietary factors. Mutagenesis. 2007;22:91-103.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 237]  [Cited by in F6Publishing: 210]  [Article Influence: 12.4]  [Reference Citation Analysis (0)]
35.  Wright RO, Schwartz J, Wright RJ, Bollati V, Tarantini L, Park SK, Hu H, Sparrow D, Vokonas P, Baccarelli A. Biomarkers of lead exposure and DNA methylation within retrotransposons. Environ Health Perspect. 2010;118:790-795.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 181]  [Cited by in F6Publishing: 126]  [Article Influence: 9.0]  [Reference Citation Analysis (0)]
36.  Yarborough A, Zhang YJ, Hsu TM, Santella RM. Immunoperoxidase detection of 8-hydroxydeoxyguanosine in aflatoxin B1-treated rat liver and human oral mucosal cells. Cancer Res. 1996;56:683-688.  [PubMed]  [DOI]  [Cited in This Article: ]
37.  Valinluck V, Tsai HH, Rogstad DK, Burdzy A, Bird A, Sowers LC. Oxidative damage to methyl-CpG sequences inhibits the binding of the methyl-CpG binding domain (MBD) of methyl-CpG binding protein 2 (MeCP2). Nucleic Acids Res. 2004;32:4100-4108.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 568]  [Cited by in F6Publishing: 546]  [Article Influence: 27.3]  [Reference Citation Analysis (0)]
38.  Bariol C, Suter C, Cheong K, Ku SL, Meagher A, Hawkins N, Ward R. The relationship between hypomethylation and CpG island methylation in colorectal neoplasia. Am J Pathol. 2003;162:1361-1371.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 122]  [Cited by in F6Publishing: 143]  [Article Influence: 6.8]  [Reference Citation Analysis (0)]
39.  Neuhausen A, Florl AR, Grimm MO, Schulz WA. DNA methylation alterations in urothelial carcinoma. Cancer Biol Ther. 2006;5:993-1001.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 38]  [Cited by in F6Publishing: 44]  [Article Influence: 2.4]  [Reference Citation Analysis (0)]