Plant Biotechnology
Online ISSN : 1347-6114
Print ISSN : 1342-4580
ISSN-L : 1342-4580
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Ti-Plasmid Vectors Useful for Functional Analysis of Rice Genes
Takuichi FUSETakuji SASAKIMasahiro YANO
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JOURNAL FREE ACCESS

2001 Volume 18 Issue 3 Pages 219-222

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Abstract

We developed new binary vectors, pPZP2H-lac and pPZP2Ha3, for the transformation of rice plants. These binary vectors contain multiple cloning sites and the hygromycin B phosphotransferase gene as a plant-selectable marker. After Agrobacterium-mediated transformation, transgenic plants containing a T-DNA could be tightly selected by hygromycin B. The vectors are powerful tools for the functional analysis of genes.

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© 2001 by Japanese Society for Plant Biotechnology
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